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Cell Labeling Methods for Noninvasive MR Imaging of Stem Cells

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Cell-Based Therapies in Stroke

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Abstract

MRI tracking of stem cells and progenitor cells is now widely being pursued in preclinical models of neurological diseases, including stroke. Typically, the cells of interest are labeled with superparamagnetic iron oxide (SPIO) particles that knock out the signal on the MR images. In this chapter, several methods are described that can be used to achieve intracellular SPIO labeling. In addition, some of the newer approaches are being discussed, in particular the use of perfluorocarbon emulsions for 19F hot spot MRI and MRI reporter genes, with the latter being the much sought-after holy grail for MRI cell tracking.

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Authors and Affiliations

  1. Division of MR Research, and Cellular Imaging Section, Russell H. Morgan Department of Radiology and Radiological Science, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, 217 Traylor Bldg, 720 Rutland Ave, Baltimore, MD, 21205-2195, USA

    Jeff W. M. Bulte Ph.D.

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  1. Jeff W. M. Bulte Ph.D.
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Correspondence to Jeff W. M. Bulte Ph.D. .

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Editors and Affiliations

  1. , Institute of Clinical Medicine, University of Eastern Finland, Kuopio, 70210, Finland

    Jukka Jolkkonen

  2. School of Medicine, Division of MR Research, Johns Hopkins University, N Broadway 733, Baltimore, 21205, Maryland, USA

    Piotr Walczak

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Bulte, J.W.M. (2013). Cell Labeling Methods for Noninvasive MR Imaging of Stem Cells. In: Jolkkonen, J., Walczak, P. (eds) Cell-Based Therapies in Stroke. Springer, Vienna. https://doi.org/10.1007/978-3-7091-1175-8_5

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  • DOI: https://doi.org/10.1007/978-3-7091-1175-8_5

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  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-1174-1

  • Online ISBN: 978-3-7091-1175-8

  • eBook Packages: MedicineMedicine (R0)

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