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Anticarbohydrate Antibodies pp 283–306Cite as

Glycan Microarray Analysis of Tumor-Associated Antibodies

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Abstract

A change in glycosylation is a common feature of tumor cells and may affect any type of glycoconjugate such as N-glycans and O-glycans on glycoproteins, and oligosaccharides on glycolipids and glycosaminoglycans (Altmann 2007; Hakomori 1985). These changes in glycan structures have marked influence on many diverse biological functions of complex carbohydrates and contribute to the malignant phenotype. Historically, changes in glycan structures on cancer cells have also served as the first biomarkers of cancer from early studies of histo-blood group antigens by immunohistochemistry (Hakomori 1984; Dabelsteen 1996; Kannagi et al. 2001) to more advanced techniques applied today involving mass spectrometry. Throughout the last 40+ years antibodies and, in particular, monoclonal antibodies (mAb) have served as a prominent tool for defining glycan changes in cancer, and a large number of antibodies to different carbohydrate structures have been developed and the fine specificities characterized by a number of different approaches (Heimburg-Molinaro and Rittenhouse-Olson 2009). In this chapter we review representative monoclonal antibodies with cancer-associated reactivity patterns and how their specificities can be analyzed by emerging microarray technologies.

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Acknowledgements

This work was supported by National Institute of General Medical Sciences Grant GM62116 to the Consortium for Functional Glycomics, The Carlsberg Foundation, The Benzon Foundation, The Velux Foundation, The Danish Research Council, The Danish Agency for Science, Technology and Innovation (FTP), NIH PO1 CA052477 NIH (1U01CA128437-01), EU FP7-HEALTH-2007-A 201381, and University of Copenhagen Programme of Excellence.

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  1. Department of Cellular and Molecular Medicine, Copenhagen Center for Glycomics, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen, Denmark

    Ola Blixt, Irene Boos & Ulla Mandel

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  1. Ola Blixt
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Correspondence to Ola Blixt .

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  1. , Department für Chemie, Universität für Bodenkultur Wien, Muthgasse 18, Wien, 1190, Austria

    Paul Kosma

  2. , Zentrum für Medizin und Biowissenschafte, Forschungszentrum Borstel, Parkallee 22, Borstel, 23845, Germany

    Sven Müller-Loennies

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Blixt, O., Boos, I., Mandel, U. (2012). Glycan Microarray Analysis of Tumor-Associated Antibodies. In: Kosma, P., Müller-Loennies, S. (eds) Anticarbohydrate Antibodies. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0870-3_12

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