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Intracerebral Hemorrhage Research pp 243–251Cite as

Pretreatment with Normobaric and Hyperbaric Oxygenation Worsens Cerebral Edema and Neurologic Outcomes in a Murine Model of Surgically Induced Brain Injury

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Part of the book series: Acta Neurochirurgica Supplementum ((NEUROCHIRURGICA,volume 111))

Abstract

Background: Hyperbaric oxygenation is a readily available treatment modality, and its ability to improve neurological outcomes in a variety of animal models has been demonstrated. This study was designed to investigate the use of a single pretreatment regimen of either hyperbaric oxygenation or normobaric oxygenation to determine its effects in a murine model of surgically induced brain injury (SBI). Materials and Methods: Hyperbaric oxygen (2.5ATM, 1 h), normobaric oxygen (100% FIO2, 1 h) or room air (21% FIO2, 1 h) was applied on CD-1 mice immediately, or at 1, 2 or 3 h followed by SBI or sham surgical operation. Neurological assessment of the animals was done by a blinded observer at 24 and 72 h using a 21-point modified Garcia scale, wire hanging test, and beam balance test. The brain edema was evaluated using brain water content at 24 and 72 h after SBI. Results: There was no statistically significant difference in the mortality rate after treatment compared with the SBI group. The brain water content after SBI was significantly increased in the right (ipsilateral) frontal lobe surrounding the site of surgical resection compared with the sham group. Both hyperbaric and normobaric oxygen treatment significantly increased the brain edema and worsened the neurological outcomes using a 21-point Garcia score compared with the SBI group. The brain edema at 24 h after injury was most pronounced in the group treated with normobaric oxygenation 2 h prior to surgery. These differences disappeared at 72 h after SBI. Conclusion: Immediate pretreatment with either hyperbaric (2.5ATM, 1 h) or normobaric oxygen (100% FIO2, 1 h) increased brain edema and worsened neurological function at 24 h following SBI.

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Acknowledgement

This study is partially supported by NIH NS053407 to J.H. Zhang.

Conflict of interest statement We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Neurosurgery, Loma Linda University Medical Center, Loma Linda, CA, USA

    David Westra, Austin Colohan & John H. Zhang

  2. Department of Physiology and Pharmacology, Loma Linda University, School of Medicine, Loma Linda, CA, USA

    Wanqiu Chen, Reiko Tsuchiyama & John H. Zhang

  3. Department of Physiology and Pharmacology, Loma Linda University, School of Medicine, Risley Hall, Room 214, Loma Linda, CA, 92350, USA

    John H. Zhang

Authors
  1. David Westra
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  2. Wanqiu Chen
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  3. Reiko Tsuchiyama
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  4. Austin Colohan
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  5. John H. Zhang
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Corresponding author

Correspondence to John H. Zhang .

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Editors and Affiliations

  1. , Neurosurgery, Physiology, Loma Linda University, Campus Street 11041, Loma Linda, 92530, USA

    John Zhang

  2. School of Medicine, Dept. Neurosurgery, Loma Linda University, Anderson St. 11234, Loma Linda, 92354, California, USA

    Austin Colohan

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© 2011 Springer-Verlag/Wien

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Westra, D., Chen, W., Tsuchiyama, R., Colohan, A., Zhang, J.H. (2011). Pretreatment with Normobaric and Hyperbaric Oxygenation Worsens Cerebral Edema and Neurologic Outcomes in a Murine Model of Surgically Induced Brain Injury. In: Zhang, J., Colohan, A. (eds) Intracerebral Hemorrhage Research. Acta Neurochirurgica Supplementum, vol 111. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0693-8_41

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  • DOI: https://doi.org/10.1007/978-3-7091-0693-8_41

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  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-0692-1

  • Online ISBN: 978-3-7091-0693-8

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