Abstract
Capsaicin, a transient receptor potential vanilloid 1 (TRPV1) agonist, has recently been shown to provide neuroprotection against brain injury in experimental adult models of cerebral ischemia. Accordingly, in this study, we investigated the way in which capsaicin-mediated TRPV1 modulation could attenuate damage in an experimental hypoxic-ischemic (HI) neonatal brain injury model. The Rice-Vannucci method was used in 10-day-old rat pups by performing unilateral carotid artery ligation followed by 2 h of hypoxia (8% O2 at 37°C). Capsaicin was administered intraperitoneally (0.2 mg/kg or 2.0 mg/kg) at 3 h pre-HI or 1 h post-HI. Post assessment included measurement of infarction volume at 24 and 72 h in addition to an assessment of the vascular dynamics of the middle cerebral artery (MCA) at 6 h post-HI. The results indicated that pre-treatment with capsaicin reduced infarction volume significantly with either low-dose or high-dose treatment. Pre-treatment also improved myogenic tone and decreased apoptotic changes in the distal MCA. We concluded that capsaicin pre-treatment may provide neurovascular protection against neonatal HI.
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Bracci R, Perrone S, Buonocore G (2006) The timing of neonatal brain damage. Biol Neonate 90:145–155
Northington FJ, Ferriero DM, Martin LJ (2001) Neurodegeneration in the thalamus following neonatal hypoxia–ischemia is programmed cell death. Dev Neurosci 23:186–191
Pedersen SF, Owsianik G, Nilius B (2005) TRP channels: an overview. Cell Calcium 38(3–4):233–252, Review
Caterina MJ (2007) Transient receptor potential ion channels as participants in thermosensation and thermoregulation. Am J Physiol Regul Integr Comp Physiol 292(1):R64–R76, Epub 2006 Sep 14. Review
Pegorini S, Braida D, Verzoni C, Guerini-Rocco C, Consalez GG, Croci L, Sala M (2005) Capsaicin exhibits neuroprotective effects in a model of transient global cerebral ischemia in Mongolian gerbils. Br J Pharmacol 144(5):727–735
Chen W, Jadhav V, Tang J, Zhang JH (2008) HIF-1alpha inhibition ameliorates neonatal brain injury in a rat pup hypoxic-ischemic model. Neurobiol Dis 31(3):433–441
Mezey E, Tóth ZE, Cortright DN, Arzubi MK, Krause JE, Elde R, Guo A, Blumberg PM, Szallasi A (2000) Distribution of mRNA for vanilloid receptor subtype 1 (VR1), and VR1-like immunoreactivity, in the central nervous system of the rat and human. Proc Natl Acad Sci USA 97(7):3655–3660
Hayes P, Meadows HJ, Gunthorpe MJ, Harries MH, Duckworth DM, Cairns W, Harrison DC, Clarke CE, Ellington K, Prinjha RK, Barton AJ, Medhurst AD, Smith GD, Topp S, Murdock P, Sanger GJ, Terrett J, Jenkins O, Benham CD, Randall AD, Gloger IS, Davis JB (2000) Cloning and functional expression of a human orthologue of rat vanilloid receptor-1. Pain 88(2):205–215
Zhong B, Wang DH (2008) N-oleoyldopamine, a novel endogenous capsaicin-like lipid, protects the heart against ischemia-reperfusion injury via activation of TRPV1. Am J Physiol Heart Circ Physiol 295(2):H728–H735
Charles SM, Zhang L, Longo LD, Buchholz JN, Pearce WJ (2007) Postnatal maturation attenuates pressure-evoked myogenic tone and stretch-induced increases in Ca2+ in rat cerebral. Am J Physiol Regul Integr Comp Physiol 293(2):R737–R744
Cristino L, de Petrocellis L, Pryce G, Baker D, Guglielmotti V, Di Marzo V (2006) Immunohistochemical localization of cannabinoid type 1 and vanilloid transient receptor potential vanilloid type 1 receptors in the mouse brain. Neuroscience 139(4):1405–1415, Epub 2006 Apr 17
Kaur C, Ling EA (2009) Periventricular white matter damage in the hypoxic neonatal brain: role of microglial cells. Prog Neurobiol 87(4):264–280
Manabat C, Han BH, Wendland M, Derugin N, Fox CK, Choi J, Holtzman DM, Ferriero DM, Vexler ZS (2003) Reperfusion differentially induces caspase-3 activation in ischemic core and penumbra after stroke in immature brain. Stroke 34(1):207–213
Scotland RS, Chauhan S, Davis C, De Felipe C, Hunt S, Kabir J, Kotsonis P, Oh U, Ahluwalia A (2004) Vanilloid receptor TRPV1, sensory C-fibers, and vascular autoregulation: a novel mechanism involved in myogenic constriction. Circ Res 95(10):1027–1034
Acknowledgement
This study is partially supported by NIH NS043338 to J.H. Zhang, and NS060936 to J. Tang. Conflict of interest statement We declare that we have no conflict of interest.
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Khatibi, N.H. et al. (2011). Capsaicin Pre-treatment Provides Neurovascular Protection Against Neonatal Hypoxic-Ischemic Brain Injury in Rats. In: Zhang, J., Colohan, A. (eds) Intracerebral Hemorrhage Research. Acta Neurochirurgica Supplementum, vol 111. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0693-8_38
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DOI: https://doi.org/10.1007/978-3-7091-0693-8_38
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