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Intracerebral Hemorrhage Research pp 151–154Cite as

Sonic Hedgehog Agonist Fails to Induce Neural Stem Cell Precursors in a Porcine Model of Experimental Intracranial Hemorrhage

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Part of the book series: Acta Neurochirurgica Supplementum ((NEUROCHIRURGICA,volume 111))

Abstract

Background: The role of endogenous neural stem cell progenitors in recovery from intracranial hemorrhage remains to be elucidated. Proliferation of such stem cells in the subventricular zone has been described in rodent models of experimental intracranial hemorrhage. Administration of a sonic hedgehog agonist at the time of hemorrhage was hypothesized to increase the quantity of such precursor cells.

Methods: Two groups of pigs were subjected to injection of autologous blood into the right frontal lobe. One group was also injected at the same site with a sonic hedgehog agonist at the time of the hemorrhage to stimulate cell growth, and the other was given a vehicle control. The pigs received intravenous BrdU for 5 days postoperatively to label replicating cells, and then were sacrificed at intervals up to 21 days.

Results: Pigs in the hemorrhage only group demonstrated increased and more persistent BrdU staining in the subventricular zone relative to pigs in the group that received sonic hedgehog agonist. The latter group demonstrated increased BrdU activity in non-neural lineage cells in the area of the hemorrhage.

Conclusion: Sonic hedgehog agonist did not induce subventricular zone neural stem cell progenitor division after experimental intracranial hemorrhage in a pig model.

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Authors and Affiliations

  1. Department of Neurosurgery, University of Rochester, New York, NY, USA

    Jing Tong & Jason H. Huang

  2. Department of Neurosurgery, New York University, 423 E. 23rd St MC 112, 4168N, New York, NY, 10010, USA

    Jonathan M. Latzman, Judah Rauch & Uzma Samadani

  3. Department of Pathology, New York University, New York, USA

    David S. Zagzag

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  1. Jing Tong
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  2. Jonathan M. Latzman
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  3. Judah Rauch
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  4. David S. Zagzag
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  5. Jason H. Huang
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  6. Uzma Samadani
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Correspondence to Uzma Samadani .

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Editors and Affiliations

  1. , Neurosurgery, Physiology, Loma Linda University, Campus Street 11041, Loma Linda, 92530, USA

    John Zhang

  2. School of Medicine, Dept. Neurosurgery, Loma Linda University, Anderson St. 11234, Loma Linda, 92354, California, USA

    Austin Colohan

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Tong, J., Latzman, J.M., Rauch, J., Zagzag, D.S., Huang, J.H., Samadani, U. (2011). Sonic Hedgehog Agonist Fails to Induce Neural Stem Cell Precursors in a Porcine Model of Experimental Intracranial Hemorrhage. In: Zhang, J., Colohan, A. (eds) Intracerebral Hemorrhage Research. Acta Neurochirurgica Supplementum, vol 111. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0693-8_25

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  • DOI: https://doi.org/10.1007/978-3-7091-0693-8_25

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  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-0692-1

  • Online ISBN: 978-3-7091-0693-8

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