Abstract
The chemical characterization of polysaccharides is an absolute request for a multitude of scientific and industrial applications that go beyond the simple use of polysaccharides where the physical characterization and the knowledge of usage-dependent behavior by specific tests are sufficient. Successful process optimization and development are today only possible by knowing and controlling the details of the molecular basis. Hence, chemical analysis of polysaccharides covers a broad range of chemical problems and structural hierarchies within the molecules. This leads to a diversity of methods necessary for a complete chemical characterization of a polysaccharide sample. This chapter reviews the main methods that can be used for performing a detailed chemical characterization of polysaccharides.
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Adden R, Mischnick P (2005) A novel method for the analysis of the substitution pattern of O-methyl-[alpha]- and [beta]-1,4-glucans by means of electrospray ionisation-mass spectrometry/collision induced dissociation. Int J Mass Spectrom 242(1):63–73
Adden R, Melander C et al (2009) The applicability of enzymes in cellulose ether analysis. Macromol Symp 280:36–44
Adorjan I, Potthast A et al (2005) Discoloration of cellulose solutions in N-methylmorpholine-N-oxide (Lyocell). Part 1: Studies on model compounds and pulps. Cellulose 12(1):51–57
Albersheim P, Nevins DJ et al (1967) A method for the analysis of sugars in plant cell-wall polysaccharides by gas–liquid chromatography. Carbohydr Res 5(3):340–345
Alexandru L, Rogovin ZA (1953) Verteilung der Thiocarbonatgru ppen zwischen den primären und sekundären Alkoholgru ppen im Cellulosexanthogenat. J Allg Chem (USSR) 23:1203–1205
Allard B, Derenne S (2009) Microwave assisted extraction and hydrolysis: an alternative to pyrolysis for the analysis of recalcitrant organic matter? Application to a forest soil (Landes de Gascogne, France). Org Geochem 40(9):1005–1017
An HJ, Franz AH et al (2003) Improved capillary electrophoretic separation and mass spectrometric detection of oligosaccharides. J Chromatogr A 1004:121–129
Andersson S-I, Samuelson O et al (1983) Structure of the reducing end-groups in spruce xylan. Carbohydr Res 111(2):283–288
Arisz PW, Kauw HJJ et al (1995) Substituent distribution along the cellulose backbone in O-methylcelluloses using GC and FAB-MS for monomer and oligomer analysis. Carbohydr Res 271(1):1–14
Bedouet L, Courtois B et al (2003) Rapid quantification of O-acetyl and O-methyl residues in pectin extracts. Carbohydr Res 338(4):379–383
Bikova T, Treimanis A (2002) Problems of the MMD analysis of cellulose by SEC using DMA/LiCl: a review. Carbohydr Polym 48(1):23–28
Black GE, Fox A (1996) Recent progress in the analysis of sugar monomers from complex matrices using chromatography in conjunction with mass spectrometry or stand-alone tandem mass spectrometry. J Chromatogr A 720(1–2):51–60
Blüher A, Vogelsanger B (2001) Mass deacidification of paper. Chimia 55:981
Bohrn R, Potthast A et al (2005) Synthesis and testing of a novel fluorescence label for carboxyls in carbohydrates and cellulosics. Synlett 20:3087–3090
Bohrn R, Potthast A et al (2006) The FDAM method: determination of carboxyl profiles in cellulosic materials by combining group-selective fluorescence labeling with GPC. Biomacromolecules 7(6):1743–1750
Bui A, Kocsis B et al (2008) Methodology to label mixed carbohydrate components by APTS. J Biochem Biophys Methods 70:1313–1316
Calvini P, Gorassini A et al (2006) FTIR and WAXS analysis of periodate oxycellulose: evidence for a cluster mechanism of oxidation. Vib Spectrosc 40(2):177–183
Campa C, Coslovi A et al (2006) Overview on advances in capillary electrophoresis-mass spectrometry of carbohydrates: a tabulated review. Electrophoresis 27:2027–2050
Capitani D, Porro F et al (2000) High field NMR analysis of the degree of substitution in carboxymethyl cellulose sodium salt. Carbohydr Polym 42(3):283–286
Chandra K, Ghosh K et al (2009) Chemical analysis of a polysaccharide of unripe (green) tomato (Lycopersicon esculentum). Carbohydr Res 344(16):2188–2194
Charlwood J, Birrell H et al (2000) A probe for the versatile analysis and characterization of N-linked oligosaccharides. Anal Chem 72:1453–1461
Chen F-TA, Evangelista RA (1995) Analysis of mono- and oligosaccharide isomers derivatized with 9-aminopyrene-1,4,6-trisulfonate by capillary electrophoresis with laser-induced fluorescence. Anal Biochem 230:273–280
Chen Y-R, Tseng M-C et al (2003) A low-flow ce/electrospray ionization MS interface for capillary zone electrophoresis, large-volume sample stacking, and micellar electrokinetic chromatography. Anal Chem 75:503–508
Chen G, Zhang L et al (2005) Determination of mannitol and three sugars in Ligustrum lucidum Ait. by capillary electrophoresis with electrochemical detection. Anal Chim Acta 530:15–21
Chen G, Zhang L et al (2006) Determination of glycosides and sugars in Moutan Cortex by capillary electrophoresis with electrochemical detection. J Pharm Biomed Anal 41:129–134
Chen Y, Xie M-Y et al (2008) Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum. Food Chem 107(1):231–241
Cheng X, Zhang S et al (2008) Determination of carbohydrates by capillary zone electrophoresis with amperometric detection at a nano-nickel oxide modified carbon paste electrode. Food Chem 106:830–835
Chiesa C, Horvath C (1993) Capillary zone electrophoresis of malto-oligosaccharides derivatized with 8-aminonaphthalene-1,3,6-trisulfonic acid. J Chromatogr A 645(2):337–352
Chiesa C, O'Neil RA et al (eds) (1996) Capillary electrophoresis in analytical biotechnology. CRC Press, Boca Raton, FL
Chu Q, Fu L et al (2005) Fast determination of sugars in Coke and Diet Coke by miniaturized capillary electrophoresis with amperometric detection. J Sep Sci 28:234–238
Ciucanu I, Kerek F (1984) A simple and rapid method for the permethylation of carbohydrates. Carbohydr Res 131(2):209–217
Cohen A, Schagerlöf H et al (2004) Liquid chromatography-mass spectrometry analysis of enzyme-hydrolysed carboxymethylcellulose for investigation of enzyme selectivity and substituent pattern. J Chromatogr A 1029(1–2):87–95
Cortacero-Ramirez S, Segura-Carretero A et al (2004) Analysis of carbohydrates in beverages by capillary electrophoresis with precolumn derivatization and UV detection. Food Chem 87:471–476
Cui SW (2005) Structural analysis of polysaccharides. In: Cui SW (ed) Food carbohydrates - chemistry, physical properties and applications. CRC Press, Boca Raton, FL, p 56
Cyrot J (1957) Dosage des fonctions oximables de la cellulose dégradée. J Chim Anal 39:449
Dabek-Zlotorzynska E, Dlouhy JF (1994) Capillary zone electrophoresis with indirect UV detection of organic anions using 2,6-naphthalenedicarboxylic acid. J Chromatogr A 685(1):145–153
Dahlman O, Jacobs A et al (2000) Analysis of carbohydrates in wood and pulps employing enzymatic hydrolysis and subsequent capillary zone electrophoresis. J Chromatogr A 891(1):157–174
De Ruiter GA, Schols HA et al (1992) Carbohydrate analysis of water-soluble uronic acid-containing polysaccharides with high-performance anion-exchange chromatography using methanolysis combined with TFA hydrolysis is superior to four other methods. Anal Biochem 207(1):176–185
Dicke R, Rahn K et al (2001) Starch derivatives of high degree of functionalization. Part 2. Determination of the functionalization pattern of p-toluenesulfonyl starch by peracylation and NMR spectroscopy. Carbohydr Polym 45(1):43–51
Doering H (1956) Determination of carboxyl groups in cellulose by complexometry. Das Papier 10:140–141
Donald AM, Buschow KHJ, et al. (2001) Polysaccharide crystallization. In: Buschow et al. (eds) Encyclopedia of materials: science and technology. Elsevier, Oxford, pp 7714–7718
Dong S, Zhang S et al (2007) Simultaneous determination of sugars and ascorbic acid by capillary zone electrophoresis with amperometric detection at a carbon paste electrode modified with polyethylene glycol and Cu2O. J Chromatogr A 1161:327–333
Dunbrant SSO (1965) Determination of primary and secondary xanthate groups in cellulose-xanthate. J Appl Polym Sci 9:2489–2499
Dupont A-L, Mortha G (2004) Comparative evaluation of size-exclusion chromatography and viscometry for the characterisation of cellulose. J Chromatogr A 1026(1–2):129–141
Ebringerova A (2006) Structural diversity and application potential of hemicelluloses. Macromol Symp 232:1–12
Ebringerova A, Hromadkova Z et al (2005) Hemicellulose. Adv Polym Sci 186:1–67
Eremeeva T (2003) Size-exclusion chromatography of enzymatically treated cellulose and related polysaccharides: a review. J Biochem Biophysl Methods 56(1–3):253–264
Eremeeva TE, Bykova TO (1998) SEC of mono-carboxymethyl cellulose (CMC) in a wide range of pH; Mark-Houwink constants. Carbohydr Polym 36(4):319–326
Erler U, Mischnick P et al (1992) Determination of the substitution patterns of cellulose methyl ethers by HPLC and GLC - comparison of methods. Polym Bull 29:349–356
France RR, Cumpstey I et al (2000) Fluorescence labelling of carbohydrates with 2-aminobenzamide (2AB). Tetrahedron Asymmetry 11(24):4985–4994
García O, Torres AL et al (2002) Effect of cellulase-assisted refining on the properties of dried and never-dried eucalyptus pulp. Cellulose 9(2):115–125
Gillespie DT, Hammons HK (1999) Analysis of polysaccharides by SEC3. In: Provder T (ed) Chromatography of Polymers, vol 731. American Chemical Society, Washington, DC, pp 288–310
Gohdes M, Mischnick P (1998) Determination of the substitution pattern in the polymer chain of cellulose sulfates. Carbohydr Res 309(1):109–115
Gohdes M, Mischnick P et al (1997) Methylation analysis of cellulose sulphates. Carbohydr Polym 33(2–3):163–168
Gonera A, Goclik V et al (2002) Preparation and structural characterisation of O-aminopropyl starch and amylose. Carbohydr Res 337(21–23):2263–2272
Gratzl JS (1985) Lichtinduzierte Vergilbund von Zellstoffen pp Ursachen und Verhütung. Das Papier 39(10A):V14–V23
Grill E, Huber C et al (1993) Capillary zone electrophoresis of p-aminobenzoic acid derivatives of aldoses, ketoses and uronic acids. Electrophoresis 14:1004–1010
Haggkvist M, Li T-Q et al (1998) Effects of drying and pressing on the pore structure in the cellulose fibre wall studied by 1 H and 2 H NMR relaxation. Cellulose 5(1):33–49
Harding SE (2005) Analysis of polysaccharides by ultracentrifugation. size, conformation and interactions in solution. In: Heinze T (ed) Polysaccharides I - structure, characterisation and use. Springer, Berlin, pp 211–254
Hase S, Ibuki T et al (1984) Reexamination of the pyridylamination used for fluorescence labeling of oligosaccharides and its application to glycoproteins. J Biochem 95:197–203
Heinrich J (1999a) Strukturaufklärung von Cellulosederivaten und Galactanen mittels chemischer, chromatographischer und massenspektrometrischer Methoden. Fachbereich Chemie, Universität Hamburg, Hamburg
Heinrich JPM (1999b) Determination of the substitution pattern in the polymer chain of cellulose acetates. J Polym Sci A Polym Chem 37(15):3011–3016
Heinze U, Heinze T et al (1999) Synthesis and structure characterization of 2,3-O-carboxymethylcellulose. Macromol Chem Phys 200(4):896–902
Heinze U, Schaller J et al (2000) Characterisation of regioselectively functionalized 2,3-O-carboxymethyl cellulose by enzymatic and chemical methods. Cellulose 7:161–175
Henniges U, Prohaska T et al (2006) A fluorescence labeling approach to assess the deterioration state of aged papers. Cellulose 13(4):421–428
Holfstetter-Kuhn S, Paulus A et al (1991) Influence of borate complexation on the electrophoretic behavior of carbohydrates in capillary electrophoresis. Anal Chem 63:1541–1547
Honda S, Suzuki S et al (1991a) Capillary zone electrophoresis of reducing mono- and oligo-saccharides as the borate complexes of their 3-methyl-1-phenyl-2-pyrazolin-5-one derivatives. Carbohydr Res 215(1):193–198
Honda S, Yamamoto K et al (1991b) High-performance capillary zone electrophoresis of carbohydrates in the presence of alkaline earth metal ions. J Chromatogr A 588(1–2):327–333
Horner S, Puls J et al (1999) Enzyme-aided characterisation of carboxymethylcellulose. Carbohydr Polym 40(1):1–7
Huber CG, Hoelzl G (eds) (2001) CapillaryElectrochromatography. Amsterdam, Elsevier
Hult E-L, Larsson PT et al (2002) A comparative CP/MAS 13C-NMR study of the supermolecular structure of polysaccharides in sulphite and kraft pulps. Holzforschung 56(2):179–184
Husemann E, Weber OH (1942) Der Carboxylgehalt von Faser- und Holzcellulosen. J Prakt Chem 159:334–342
Issaq HJ, Janini GM et al (2004) Sheathless electrospray ionization interfaces for capillary electrophoresis–mass spectrometric detection. Advantages and limitations. J Chromatogr A 1053:37–42
Ivanov AR, Nazimov IV et al (2000) Direct determination of amino acids and carbohydrates by high-performance capillary electrophoresis with refractometric detection. J Chromatogr A 894:253–257
Jager AV, Tonin FG et al (2007) Comparative evaluation of extraction procedures and method validation for determination of carbohydrates in cereals and dairy products by capillary electrophoresis. J Sep Sci 30:586–594
Kabel MA (2002) Characterisation of complex xylo-oligosaccharides from xylan rich by-products. PhD thesis. Department of Agrotechnology and Food Sciences, Wageningen University, Wageningen
Kabel MA, de Waard P et al (2003) Location of O-acetyl substituents in xylo-oligosaccharides obtained from hydrothermally treated Eucalyptus wood. Carbohydr Res 338(1):69–77
Kato KL, Cameron RE (1999) A review of the relationship between thermally-accelerated ageing of paper and hornification. Cellulose 6(1):23–40
Katzenellenbogen E, Kocharova NA et al (2009) Structure of an abequose-containing O-polysaccharide from Citrobacter freundii O22 strain PCM 1555. Carbohydr Res 344(13):1724–1728
Kelli JF, Ramaley L et al (1997) Capillary zone electrophoresis-electrospray mass spectrometry at submicroliter flow rates: practical considerations and analytical performance. Anal Chem 69:51–60
Kiwitt-Haschemie K, Renger A et al (1996) A comparison between reductive-cleavage and standard methylation analysis for determining structural features of galactomannans. Carbohydr Polym 30(1):31–35
Klampfl C (2006) Recent advances in the application of capillary electrophoresis with mass spectrometric detection. Electrophoresis 27:3–34
Klampfl CW, Buchberger W (2001) Determination of carbohydrates by capillary electrophoresis with electrospray-mass spectrometric detection. Electrophoresis 22(13):2737–2742
Klemm D, Philipp B et al (1998) Comprehensive cellulose chemistry, vol 1, Fundamentals and analytical methods. Wiley-VCH, Weinheim
Klockow A, Amado R et al (1995) Separation of 8-aminonaphthalene-l,3,6-trisulfonic acid-labelled neutral and sialylated N-linked complex oligosaccharides by capillary electrophoresis. J Chromatogr A 716:241–257
Klockow A, Amado R et al (1996) The influence of buffer composition on separation efficiency and resolution in capillary electrophoresis of 8-aminonaphthalene-1,3,6-trisulfonic acid labeled monosaccharides and complex carbohydrates. Electrophoresis 17:110–119
Koelhed M, Karlberg B (2005) Capillary electrophoretic separation of sugars in fruit juices using on-line mid infrared Fourier transform detection. Analyst 130:772–778
Kondo A, Suzuki J et al (1990) Improved method for fluorescence labeling of sugar chains with sialic acid residues. Agric Biol Chem 54(8):2169–2170
Krainz K, Potthast A et al (2009) Effects of selected key chromophores on cellulose integrity upon bleaching 10th EWLP, Stockholm, Sweden, August 25-28, 2008. Holzforschung 63(6):647–655
Kristiansen KA, Ballance S et al (2009) An evaluation of tritium and fluorescence labelling combined with multi-detector SEC for the detection of carbonyl groups in polysaccharides. Carbohydr Polym 76(2):196–205
Kumirska J, Czerwicka M et al (2010) Application of spectroscopic methods for structural analysis of chitin and chitosan. Mar Drugs 8(5):1567–1635
Laine CTTVAVT (2002) Methylation analysis as a tool for structural analysis of wood polysaccharides. Holzforschung 56(6):607–614
Larsson PT, Wickholm K et al (1997) A CP/MAS 13C NMR investigation of molecular ordering in celluloses. Carbohydr Res 302:19–25
Lee CK, Gray GR (1995) Analysis of positions of substitution of O-acetyl groups in partially O-acetylated cellulose by the reductive-cleavage method. Carbohydr Res 269(1):167–174
Lee S-J, Altaner C et al (2003) Determination of the substituent distribution along cellulose acetate chains as revealed by enzymatic and chemical methods. Carbohydr Polym 54(3):353–362
Levigne S, Thomas M et al (2002) Determination of the degrees of methylation and acetylation of pectins using a C18 column and internal standards. Food Hydrocolloids 16(6):547–550
Lewin L (1972) In: Whistler RL, BeMiller JN (eds) Methods in carbohydrate chemistry, vol 6. Academic, New York, p 76
Lewin M (1997) Oxidation and aging of cellulose. Macromol Symp 118:715–724
Li DT, Sheen JF et al (2000) Structural analysis of chromophore-labeled disaccharides by capillary electrophoresis tandem mass spectrometry using ion trap mass spectrometry. J Am Soc Mass Spectrom 11:292–300
Li J, Wan Y et al (2009) Preparation and characterization of 2,3-dialdehyde bacterial cellulose for potential biodegradable tissue engineering scaffolds. Mater Sci Eng C 29(5):1635–1642
Liebert T, Pfeiffer K et al (2005) Carbamoylation applied for structure determination of cellulose derivatives. Macromol Symp 223(1):93–108
Liitiä T, Maunu SL et al (2000) Solid state NMR studies on cellulose crystallinity in fines and bulk fibres separated from refined kraft pulp. Holzforschung 54(6):618–624
Liu J, Shirota O et al (1991) Capillary electrophoresis of amino sugars with laser-induced fluorescence detection. Anal Chem 63:413–417
Liu Y, Shu C et al (1997) High-performance capillary electrophoretic separation of carbohydrates with indirect UV detection using diethylamine and borate as electrolyte additives. J Capillary Electrophor 4(3):97–103
Manelius R, Buléon A et al (2000) The substitution pattern in cationised and oxidised potato starch granules. Carbohydr Res 329(3):621–633
Mazumder S, Lerouge P et al (2005) Structural characterisation of hemicellulosic polysaccharides from Benincasa hispida using specific enzyme hydrolysis, ion exchange chromatography and MALDI-TOF mass spectroscopy. Carbohydr Polym 59(2):231–238
Mazzarino M, De Angelis F et al (2010) Microwave irradiation for a fast gas chromatography–mass spectrometric analysis of polysaccharide-based plasma volume expanders in human urine. J Chromatogr B 878(29):3024–3032
Mechref Y, Ostrander GK et al (1995) Capillary electrophoresis of carboxylated carbohydrates. Part 2. Selective precolumn derivatization of sialooligosaccharides derived from gangliosides with 7-aminonaphthalene-l,3-disulfonic acid fluorescing tag. Electrophoresis 16:1499–1504
Mechref Y, Ostrander GK et al (1997) Capillary electrophoresis of carboxylated carbohydrates. IV. Adjusting the separation selectivity of derivatized carboxylated carbohydrates by controlling the electrolyte ionic strength at subambient temperature and in the absence of electroosmotic flow. J Chromatogr A 792:75–82
Melander C, Tømmeraas K (2010) Heterogeneous hydrolysis of hyaluronic acid in ethanolic HCl slurry. Carbohydr Polym 82(3):874–879
Mihranyan A, Llagostera AP et al (2004) Moisture sorption by cellulose powders of varying crystallinity. Int J Pharm 269(2):433–442
Mischnick P (1991) Determination of the substitution pattern of cellulose acetates. J Carbohydr Chem 10(4):711–722
Mischnick P (1997) New developments in the analysis of the substitution pattern of polysaccharide derivatives. Macromol Symp 120:281–290
Mischnick P (2001) Challenges in structure analysis of polysaccharide derivatives. Cellulose 8(4):245–257
Mischnick P (2002) Challenges in structure analysis of polysaccharide derivatives. Cellulose 00:1–13
Mischnick P, Adden R (2008) Fractionation of polysaccharide derivatives and subsequent analysis to differentiate heterogeneities on various hierarchical levels. Macromol Symp 262:1–7
Mischnick P, Hennig C (2001) A new model for the substitution patterns in the polymer chain of polysaccharide derivatives. Biomacromolecules 2(1):180–184
Mischnick P, Evers B et al (1994) Analysis of oligosaccharides containing 2-deoxy-alpha-D-arabino-hexosyl residues by the reductive-cleavage method. Carbohydr Res 264(2):293–304
Mischnick P, Heinrich J et al (2000) Structure analysis of 1,4-glucan derivatives. Macromol Chem Phys 201:1985–1995
Mischnick P, Niedner W et al (2005) Possibilities of mass spectrometry and tandem-mass spectrometry in the analysis of cellulose ethers. Macromol Symp 223(1):67–78
Mischnick P, Momcilovic D et al (2010) Chemical structure analysis of starch and cellulose derivatives. Adv Carbohydr Chem Biochem 64:117–210
Momenbeik F, Johns C et al (2006) Sensitive determination of carbohydrates labelled with p-nitroaniline by capillary electrophoresis with photometric detection using a 406 nm light-emitting diode. Electrophoresis 27:4039–4046
Mort AJ, Chen EMW (1996) Separation of 8-aminonaphthalene-l,3,6-trisulfonate (ANTS)-labeled oligomers containing galacturonic acid by capillary electrophoresis: application to determining the substrate specificity of endopolygalacturonases. Electrophoresis 17:379–383
Mukerjea R, Kim D et al (1996) Simplified and improved methylation analysis of saccharides, using a modified procedure and thin-layer chromatography. Carbohydr Res 292:11–20
Newman RH, Davidson TC (2004) Molecular conformations at the cellulose-water interface. Cellulose 11:23–32
Nguyen DT, Lerch H et al (1997) Separation of derivatized carbohydrates by co-electroosmotic capillary electrophoresis. Chromatographia 46(3/4):113–121
Nie S-P, Xie M-Y (2011) A review on the isolation and structure of tea polysaccharides and their bioactivities. Food Hydrocolloids 25(2):144–149
Oh SY, Yoo DI et al (2005) FTIR analysis of cellulose treated with sodium hydroxide and carbon dioxide. Carbohydr Res 340(3):417–428
Osborn HMI, Lochey F et al (1999) Analysis of polysaccharides and monosaccharides in the root mucilage of maize (Zea mays L.) by gas chromatography. J Chromatogr A 831(2):267–276
Oudhoff KA, Buijtenhuijs FA et al (2004) Determination of the degree of substitution and its distribution of carboxymethylcelluloses by capillary zone electrophoresis. Carbohydr Res 339(11):1917–1924
Perera A, Meda V et al (2010) Resistant starch: a review of analytical protocols for determining resistant starch and of factors affecting the resistant starch content of foods. Food Res Int 43(8):1959–1974
Petzold K, Schwikal K et al (2006) Carboxymethyl xylan - synthesis and detailed structure characterization. Carbohydr Polym 64(2):292–298
Phillipp B, Rehder W et al (1965) Carboxylgruppenbestimmung in Chemiezellstoffen. Das Papier 19:1–9
Plocek J, Chmelik J (1997) Separation of disaccharides as their borate complexes by capillary electrophoresis with indirect detection in visible range. Electrophoresis 18:1148–1152
Potthast A, Röhrling J et al (2003) A novel method for the determination of carbonyl groups in cellulosics by fluorescence labeling. 3. Monitoring oxidative processes. Biomacromolecules 4(3):743–749
Potthast A, Schiehser S et al (2004) Effect of UV radiation on the carbonyl distribution in different pulps. Holzforschung 58(6):597–602
Potthast A, Rosenau T et al (2006) Analysis of oxidized functionalities in cellulose. In: Klemm D (ed) Polysaccharides II. Springer, Heidelber, pp 1–48
Putnam ES (1964) The exchange reaction between calcium and carboxyl groups in cellulose. TAPPI J 47:549–554
Racaityte K, Kiessig S et al (2005) Application of capillary zone electrophoresis and reversed-phase high-performance liquid chromatography in the biopharmaceutical industry for the quantitative analysis of the monosaccharides released from a highly glycosylated therapeutic protein. J Chromatogr A 1079:354–365
Rana V, Kumar V et al (2009) Structure of the oligosaccharides isolated from Dalbergia sissoo Roxb. leaf polysaccharide. Carbohydr Polym 78(3):520–525
Rehder W, Philipp B et al (1965) Ein Beitrag zur Analytik der Carbonylgru ppen in Oxycellulosen und technischen Zellstoffen. Das Papier 19(9):502
Ren J-L, Sun R-C (2010) Hemicelluloses. In: Sun R-C (ed) Cereal straw as a resource for sustainable biomaterials and biofuels. Elsevier, Amsterdam, pp 73–130
Ristolainen M (1999) Characterization of totally chlorine-free effluents from Kraft pulp bleaching II. Analysis of carbohydrate-derived constituents after acid hydrolysis by capillary zone electrophoresis. J Chromatogr A 832:203–209
Röder T, Moosbauer J et al (2006a) Crystallinity determination of man-made cellulose fibers – comparison of analytical methods. Lenzinger Ber 86:132–136
Röder T, Moosbauer J et al (2006b) Crystallinity determination of native cellulose – comparison of analytical methods. Lenzinger Ber 86:85–89
Röder T, Moosbauer J et al (2009) Comparative characterisation of man-made regenerated cellulose fibres. Lenzinger Ber 87:98–105
Röhrling J, Potthast A et al (2001) Synthesis and testing of a novel fluorescence label for carbonyls in carbohydrates and cellulosics. Synlett 5:682–684
Röhrling J, Potthast A et al (2002a) A novel method for the determination of carbonyl groups in cellulosics by fluorescence labeling. 2. Validation and applications. Biomacromolecules 3(5):969–975
Röhrling J, Potthast A et al (2002b) A novel method for the determination of carbonyl groups in cellulosics by fluorescence labeling. 1. Method development. Biomacromolecules 3(5):959–968
Rosenau T, Potthast A et al (2001) Hydrolytic processes and condensation reactions in the cellulose solvent system N, N-dimethylacetamide/lithium chloride. Part 1. Holzforschung 55(6):661–666
Rosenau T, Potthast A et al (2004) Isolation and identification of residual chromophores in cellulosic materials. Polymer 45(19):6437–6443
Rosenau T, Potthast A et al (2005a) Isolation and identification of residual chromophores in cellulosic materials, vol 223. Wiley-VCH, Weinheim, pp 239–252
Rosenau T, Potthast A et al (2005b) Discoloration of cellulose solutions in N-methylmorpholine-N-oxide (Lyocell). Part 2: Isolation and identification of chromophores. Cellulose 12(2):197–208
Rosenau T, Potthast A et al (2007) Isolation and identification of residual chromophores from aged bleached pulp samples. Holzforschung 61:656–661
Rovio S, Yli-Kauhaluoma J et al (2007) Determination of neutral carbohydrates by CZE with direct UV detection. Electrophoresis 28:3129–3135
Rovio S, Simolin H et al (2008) Determination of monosaccharide composition in plant fiber materials by capillary zone electrophoresis. J Chromatogr A 1185:139–144
Rovio S, Siren K et al (2011) Application of capillary electrophoresis to determine metal cations, anions, organic acids, and carbohydrates in some Pinot Noir red wines. Food Chem 124:1194–1200
Ruiz-Matute AI, Hernández-Hernández O et al (2010) Derivatization of carbohydrates for GC and GC-MS analyses. J Chromatogr B Analyt Technol Biomed Life Sci 879(17–18):1226–1240
Rußler A, Lange T et al (2005a) A novel method for analysis of xanthate group distribution in viscoses. Macromol Symp 223(1):189–200
Russler A, Potthast A, et al (2005a) New methylation analysis of viscose. Institute of Chemistry, Slovak Academy of Sciences: 13th European carbohydrate symposium, 21–26 August, Bratislava; Book of Abstracts, Institute of Chemistry, Slovak Academy of Sciences
Russler A, Saake B, et al (2005b) A novel approach to assess xanthate group distribution in viscose. Japanese-European workshop on cellulose and functional polysaccharides. Department of Chemistry, University of Natural Resources and Applied Life Sciences, Vienna, p 74
Rußler A, Potthast A et al (2006) Determination of substituent distribution of viscoses by GPC. Holzforschung 60(5):467–473
Rydlund A, Dahlman O (1996) Efficient capillary zone electrophoretic separation of wood-derived neutral and acidic mono- and oligosaccharides. J Chromatogr A 738:129–140
Rydlund A, Dahlman O (1997) Oligosaccharides obtained by enzymatic hydrolysis of birch kraft pulp xylan: analysis by capillary zone electrophoresis and mass spectrometry. Carbohydr Res 30:95–102
Saake B, Horner S et al (2000) Detailed investigation on the molecular structure of carboxymethyl cellulose with unusual substitution pattern by means of an enzyme-supported analysis. Macromol Chem Phys 201(15):1996–2002
Saake B, Kruse T et al (2001) Investigation on molar mass, solubility and enzymatic fragmentation of xylans by multi-detected SEC chromatography. Bioresour Technol 80(3):195–204
Sanz ML, Martínez-Castro I (2007) Recent developments in sample preparation for chromatographic analysis of carbohydrates. J Chromatogr A 1153(1–2):74–89
Sartori J, Potthast A et al (2003) Alkaline degradation kinetics and CE-separation of cello- and xylooligomers. Part I. Carbohydr Res 338:1209–1216
Sato H, Mizutani S-I et al (2002) Determination of degree of substitution in N-carboxyethylated chitin derivatives by pyrolysis-gas chromatography in the presence of oxalic acid. J Anal Appl Pyrolysis 64(2):177–185
Schelosky N, Röder T et al (1999) Molmasseverteilug cellulosischer Produkte mittels Grössenausschlusschromatographie in DMAc/LiCl. Das Papier 53(12):728–738
Schleicher H, Lang H (1994) Carbonyl and carboxyl groups in pulps and cellulose products. Das Papier 12:765–768
Schwaiger H, Oefner PJ et al (1994) Capillary zone electrophoresis and micellar electrokinetic chromatography of 4-aminobenzonitrile carbohydrate derivatives. Electrophoresis 15:941–952
Schwikal K, Heinze T et al (2005) Cationic xylan derivatives with high degree of functionalization. Macromol Symp 232(1):49–56
Senso A, Franco P et al (2000) Characterization of doubly substituted polysaccharide derivatives. Carbohydr Res 329(2):367–376
Singh V, Tiwari A et al (2006) Microwave-promoted hydrolysis of plant seed gums on alumina support. Carbohydr Res 341(13):2270–2274
Sixta H (1995). Habilitation thesis, Zellstoffherstellung unter Berücksichtigung umweltfreundlicher, Aufschluß- und Bleichverfahren am Beispiel von Chemiezellstoffen. Technical University of Graz, Graz
Sjöberg J, Adorjan I et al (2004) An optimized CZE method for analysis of mono- and oligomeric aldose mixtures. Carbohydr Res 339:2037–2043
Snyder DS, Gibson D et al (2006) Structure of a capsular polysaccharide isolated from Salmonella enteritidis. Carbohydr Res 341(14):2388–2397
Soga T, Heiger DN (1998) Simultaneous determination of monosaccharides in glycoproteins by capillary electrophoresis. Anal Biochem 261:73–78
Soga T, Ross GA (1999) Simultaneous determination of inorganic anions, organic acids, amino acids and carbohydrates by capillary electrophoresis. J Chromatogr A 837:231–239
Soga T, Serwe M (2000) Determination of carbohydrates in food samples by capillary electrophoresis with indirect UV detection. Food Chem 69:339–344
Steeneken PAM, Woortman AJJ (1994) Substitution patterns in methylated starch as studied by enzymic degradation. Carbohydr Res 258:207–221
Stefansson M, Novotny M (1994) Separation of complex oligosaccharide mixtures by capillary electrophoresis in the open-tubular format. Anal Chem 66:1134–1140
Sun Y-X, Liu J-C et al (2010) Purification, composition analysis and antioxidant activity of different polysaccharide conjugates (A PPs) from the fruiting bodies of Auricularia polytricha. Carbohydr Polym 82(2):299–304
Szabolcs O (1961) A colorimetric method for the determination of reducing carbonyl groups in cellulose. Das Papier 15:41
TAPPI (2009) T 249 - Carbohydrate composition of extractive-free wood and wood pulp by gas–liquid chromatography, p 8
TAPPI (2009) TAPPI method T-430 om-99 Copper number of pulp, paper, and paperboard (Braidy)
Thomas M, Chauvelon G et al (2003) Location of sulfate groups on sulfoacetate derivatives of cellulose. Carbohydr Res 338(8):761–770
Tüting W, Adden R et al (2004a) Fragmentation pattern of regioselectively O-methylated maltooligosaccharides in electrospray ionisation-mass spectrometry/collision induced dissociation. Int J Mass Spectrom 232(2):107–115
Tüting W, Wegemann K et al (2004b) Enzymatic degradation and electrospray tandem mass spectrometry as tools for determining the structure of cationic starches prepared by wet and dry methods. Carbohydr Res 339(3):637–648
Vaca-Garcia C, Borredon ME et al (2001) Determination of the degree of substitution (DS) of mixed cellulose esters by elemental analysis. Cellulose 8(3):225–231
van der Burgt YEM, Bergsma J et al (1998) Distribution of methyl substituents over branched and linear regions in methylated starches. Carbohydr Res 312(4):201–208
van der Burgt YEM, Bergsma J et al (1999) Distribution of methyl substituents over crystalline and amorphous domains in methylated starches. Carbohydr Res 320(1–2):100–107
van der Burgt YE, Bergsma J et al (2000a) Substituent distribution in highly branched dextrins from methylated starches. Carbohydr Res 327(4):423–429
van der Burgt YEM, Bergsma J et al (2000b) Distribution of methyl substituents in amylose and amylopectin from methylated potato starches. Carbohydr Res 325(3):183–191
van der Burgt YEM, Bergsma J et al (2000c) FAB CIDMS/MS analysis of partially methylated maltotrioses derived from methylated amylose: a study of the substituent distribution. Carbohydr Res 329:341–349
Vlasenko EY, Ryan AI et al (1998) The use of capillary viscometry, reducing end-group analysis, and size exclusion chromatography combined with multi-angle laser light scattering to characterize endo-1,4-[beta]-glucanases on carboxymethylcellulose: a comparative evaluation of the three methods. Enzym Microb Technol 23(6):350–359
Walter RH (1998) Isolation, purification, and characterization. In: Steve T (ed) Polysaccharide dispersions. Academic, San Diego, CA, Chapter 7, pp 123–155
Wang X, Chen Y (2001) Determination of carbohydrates as their p-sulfophenylhydrazones by capillary zone electrophoresis. Carbohydr Res 332:191–196
Wang X, Chen Y et al (2002) Analysis of carbohydrates by capillary zone electrophoresis with on-line capillary derivatization. J Liq Chrom Rel Technol 25(4):589–600
Wennerblom A (1961) Determination of carbonyl groups in hydrocellulose. Sven Pap 14:519
Wickholm K, Larsson PT et al (1998) Assignment of non-crystalline forms in cellulose I by CP/MAS 13C NMR spectroscopy. Carbohydr Res 312:123–129
Wilke O, Mischnick P (1995) Analysis of cationic starches: determination of the substitution pattern of O-(2-hydroxy-3-trimethylammonium)propyl ethers. Carbohydr Res 275(2):309–318
Willför S, Sundberg K et al (2008) Spruce-derived mannans - a potential raw material for hydrocolloids and novel advanced natural materials. Carbohydr Polym 72(2):197–210
Willför S, Pranovich A et al (2009) Carbohydrate analysis of plant materials with uronic acid-containing polysaccharides-A comparison between different hydrolysis and subsequent chromatographic analytical techniques. Ind Crop Prod 29(2–3):571–580
Wilson K (1948) Determination of carboxylic groups in pulp. Sven Pap 51:45–49
Wittgren B, Porsch B (2002) Molar mass distribution of hydroxypropyl cellulose by size exclusion chromatography with dual light scattering and refractometric detection. Carbohydr Polym 49(4):457–469
Yamamoto K, Hamase K et al (2003) 2-Amino-3-phenylpyrazine, a sensitive fluorescence prelabeling reagent for the chromatographic or electrophoretic determination of saccharides. J Chromatogr A 1004(1–2):99–106
Yang L, Wang Z et al (2010) Isolation and structural characterization of a polysaccharide FCAP1 from the fruit of Cornus officinalis. Carbohydr Res 345(13):1909–1913
You J, Sheng X et al (2008) Detection of carbohydrates using new labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone by capillary zone electrophoresis with absorbance (UV). Anal Chim Acta 609:66–75
Yu N, Gray GR (1998a) Analysis of the positions of substitution of acetate and butyrate groups in cellulose acetate-butyrate by the reductive-cleavage method. Carbohydr Res 312(4):225–231
Yu N, Gray GR (1998b) Analysis of the positions of substitution of acetate and propionate groups in cellulose acetate-propionate by the reductive-cleavage method. Carbohydr Res 313(1):29–36
Zamfir A, Peter-Katalinic J (2004) Capillary electrophoresis-mass spectrometry for glycoscreening in biomedical research. Electrophoresis 25:1949–1963
Zatkovskis Carvalho A, da Silva JAF et al (2003) Determination of mono- and disaccharides by capillary electrophoresis with contactless conductivity detection. Electrophoresis 24:2138–2143
Zemann A, Nguyen DT et al (1997) Fast separation of underivatized carbohydrates by coelectroosmotic capillary electrophoresis. Electrophoresis 18:1142– 1147
Zhou W, Baldwin RP (1996) Capillary electrophoresis and electrochemical detection of underivatized oligo- and polysaccharides with surfactant-controlled electroosmotic flow. Electrophoresis 17:319–324
Zuckerstätter G, Schild G et al (2009) The elucidation of cellulose supramolecular structure by 13C CP-MAS NMR. Lenzinger Ber 87:41–49
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Rußler, A., Bogolitsyna, A., Zuckerstätter, G., Potthast, A., Rosenau, T. (2012). Chemical Characterization of Polysaccharides. In: Navard, P. (eds) The European Polysaccharide Network of Excellence (EPNOE). Springer, Vienna. https://doi.org/10.1007/978-3-7091-0421-7_4
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