Abstract
The purpose of the present study was to observe the proliferation of cerebral arterial smooth muscle cell (CASMC) induced by oxyhemoglobin (Oxyhb) and interfered by Adenovirus-mediate-PKGI (Ad-PKGI), and to investigate the potential regulative role of the PKGI gene in the molecule mechanism of cerebral vasospasm (CVS) after Subarachnoid hemorrhage (SAH). Tissue-sticking method was used for primary cultured rat CASMCs. Semi-quantitative reverse transcription and polymerase chain reaction (RT-PCR) and western blot were used to examine the PKGI mRNA and protein expressions after CASMC were transfected by Ad-PKG. The proliferation of CASMCs was determined by MTT assay and 3H-TdR incorporation. Ad-PKGI could be transfected into CASMCS and highly express. Oxyhemoglobin could stimulate the proliferation of CASMC; the value of 3H-TdR incorporation and the absorbance value of MTT increased and could block up after CASMC was transfected by Ad-PKG. The results suggested that the PKG signaling pathway might play an important role in CVS after SAH, and the PKG gene might be a target point of gene therapy.
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Notes
- 1.
Chunxia Luo and Bin Yi contributed equally to this study
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Acknowledgements
This research job is supported by Grant No. 30700347, 30500662, 30772224 and 30801186 from the National Science Foundation of China (NSFC) and Grant No.2007BB5045 from the Natural Science Foundation of Chongqing, China.
Conflict of interest statement We declare that we have no conflict of interest.
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Luo, C. et al. (2011). PKGIα Inhibits the Proliferation of Cerebral Arterial Smooth Muscle Cell Induced by Oxyhemoglobin After Subarachnoid Hemorrhage1 . In: Feng, H., Mao, Y., Zhang, J.H. (eds) Early Brain Injury or Cerebral Vasospasm. Acta Neurochirurgica Supplements, vol 110/1. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0353-1_29
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