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Nascent peptide-mediated ribosome stalling promoted by antibiotics

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Ribosomes

Abstract

Many mechanisms that regulate protein expression operate through mRNA. The sequence of the Shine-Dalgarno region and its accessibility influence the efficiency of initiation of translation; the choice of codons and mRNA secondary structure affect progression of the ribosome along mRNA at the elongation stage; and the mRNA context and identity of the stop codon determine how efficiently protein is released at the termination step. However, there is another important mechanism that controls expression of a number of genes at a principally different level. In this mechanism, the ribosome checks the structure of the polypeptide it is assembling; in response to certain nascent peptide sequences and, often, specific cellular cues, it modulates its activity. One of the most dramatic types of ribosomal response to the regulatory nascent peptide sequences is stalling. In the best-characterized cases, the nascent peptide—controlled stalling occurs at a dedicated regulatory open reading frame (ORF) that precedes the regulated gene or operon whose expression is transcriptionally or translationally attenuated. Ribosome stalling relieves the attenuation by either altering the mRNA secondary structure or interfering with binding of the transcription termination factors.

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Authors
  1. Nora Vázquez-Laslop
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  2. Haripriya Ramu
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  3. Alexander Mankin
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Editors and Affiliations

  1. Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry, 37077, Göttingen, Germany

    Marina V. Rodnina  & Wolfgang Wintermeyer  & 

  2. Howard Hughes Medical Institute Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA

    Rachel Green

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Vázquez-Laslop, N., Ramu, H., Mankin, A. (2011). Nascent peptide-mediated ribosome stalling promoted by antibiotics. In: Rodnina, M.V., Wintermeyer, W., Green, R. (eds) Ribosomes. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0215-2_30

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