Abstract
In the bacterial cell there is an ever evolving network of metabolic pathways, with well-defined flow-stoichiometries for maximal yield of biomass in different growth contexts (Ibarra et al., 2002; Feist et al., 2009). In line with this, any change in size or kinetic efficiency of an enzyme or macromolecular complex like the ribosome will affect the growth rate of the cell (Ehrenberg and Kurland, 1984; Kurland et al., 2003). When the intracellular control systems maintain optimal flow couplings for maximal growth rate, there is a simple relation between change in size or kinetic efficiency of an enzyme system and the bacterial growth rate. This can be used to assess the fitness loss or gain of mutations in enzyme systems and thus the probability of fixation of gain of function mutations (Kurland et al., 2003). With μ 0 defined as the wild type (exponential) growth rate, μ the altered growth rate in a mutant, δμ = μ − μ 0 and s the fitness parameter, we have μ = μ 0 (1+s), where s = δμ/μ0.
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Johansson, M., Ieong, K.W., Åqvist, J., Pavlov, M.Y., Ehrenberg, M. (2011). Rate and accuracy of messenger RNA translation on the ribosome. In: Rodnina, M.V., Wintermeyer, W., Green, R. (eds) Ribosomes. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0215-2_18
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DOI: https://doi.org/10.1007/978-3-7091-0215-2_18
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