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The mechanism of ribosome recycling in eukaryotes

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Ribosomes

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Abstract

Protein synthesis is a cyclical process, consisting of initiation, elongation, termination and ribosome recycling stages. Initiation requires pools of separated small and large ribosomal subunits, and it has been known for about forty years that, after termination, eukaryotic 80S ribosomes dissociate from polysomes into 40S and 60S subunits, which then either participate in the next round of initiation, or enter a reservoir of stable, translationally inactive 80S monosomes (Adamson et al., 1969; Hogan and Korner, 1968b; Kaempfer 1969; Falvey and Staehelin, 1970; Howard et al., 1970; Henshaw et al., 1973). Although 80S monosomes accumulate in response to stresses that inhibit initiation, such as amino acid or glucose starvation, reversal of these conditions allows them to be dissociated and their subunits to re-enter the translation process (Hogan and Korner, 1968 a). In bacteria, recycling of post-termination 70S ribosomal complexes (post-TCs) requires elongation factor EF-G, the dedicated ribosome recycling factor RRF (Hirashima and Kaji, 1973) and initiation factor IF3.

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  1. Andrey V. Pisarev
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  2. Maxim A. Skabkin
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  3. Vera P. Pisareva
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  4. Olga V. Skabkina
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  5. Christopher U. T. Hellen
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  6. Tatyana V. Pestova
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Editors and Affiliations

  1. Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry, 37077, Göttingen, Germany

    Marina V. Rodnina  & Wolfgang Wintermeyer  & 

  2. Howard Hughes Medical Institute Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA

    Rachel Green

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Pisarev, A.V., Skabkin, M.A., Pisareva, V.P., Skabkina, O.V., Hellen, C.U.T., Pestova, T.V. (2011). The mechanism of ribosome recycling in eukaryotes. In: Rodnina, M.V., Wintermeyer, W., Green, R. (eds) Ribosomes. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0215-2_14

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