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Field-Flow Fractionation for Assessing Biomolecular Interactions in Solution

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Book cover Field-Flow Fractionation in Biopolymer Analysis

Abstract

Many biological systems are primarily governed by protein-protein interactions. It is important to develop sensitive analytical techniques to identify and characterize these bimolecular interactions in order to understand their fundamental roles in biological processes and in disease. In this book chapter, we summarize three case studies that applied asymmetrical flow field-flow fractionation (AF4) to access the protein-protein interactions of therapeutic proteins with their counterparts. These new applications of AF4 provide a unique and innovative tool that extends the bioanalytical capability to study protein complexes beyond micro-molar affinity.

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Author information

Authors and Affiliations

  1. Department of Formulation & Analytical Resources, Amgen Inc, Thousand Oaks, CA, USA

    Robert Y. -T. Chou, Joey Pollastrini, Thomas M. Dillon, Pavel V. Bondarenko & Shawn Cao

  2. Amgen Inc, Thousand Oaks, CA, USA

    Robert Y. -T. Chou

  3. Department of Protein Science, Amgen Inc, Thousand Oaks, CA, USA

    Lei-Ting T. Tam

  4. Department of Clinical Immunology, Amgen Inc, Thousand Oaks, CA, USA

    Jill Miller & Michael Moxness

Authors
  1. Robert Y. -T. Chou
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  2. Joey Pollastrini
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  3. Thomas M. Dillon
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  4. Pavel V. Bondarenko
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  5. Lei-Ting T. Tam
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  6. Jill Miller
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  7. Michael Moxness
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  8. Shawn Cao
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Corresponding author

Correspondence to Robert Y. -T. Chou .

Editor information

Editors and Affiliations

  1. Colorado School of Mines, Golden Colorado, 80401, Colorado, USA

    S. Kim R. Williams

  2. Department of Physical and Analytical Chemistry, Section of, Uppsala, 751 23, Sweden

    Karin D. Caldwell

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© 2012 Springer-Verlag/Wien

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Chou, R.YT. et al. (2012). Field-Flow Fractionation for Assessing Biomolecular Interactions in Solution. In: Williams, S., Caldwell, K. (eds) Field-Flow Fractionation in Biopolymer Analysis. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0154-4_8

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