Abstract
Previous in vitro investigations have shown that Photofrin II (dihematoporphyrinester and -ether) reduces proliferative activity and cellular vitality of cultured human smooth muscle cells from atherosclerotic plaques with and without photoactivation. The effects of Photofrin II were tested on endothelial cell cultures from human saphenous veins as to proliferative activity, cellular vitality and cell morphology. Incubation with Photofrin II without photoactivation for 3, 6 and 9 days resulted in a considerable decrease of proliferation only at a concentration of 5 μg/ml or more. At his concentration of 5 μg/ml, additional photoactivation caused light-dose dependent morphological changes and a decrease in the number of viable cells. Smooth muscle cells form atherosclerotic plaques, however, reacted with higher sensitivity to photodynamic treatment. The in vitro results suggest that a photodynamic therapy for atherosclerosis could be promising.
Zusammenfassung
Frühere in-vitro-Untersuchungen haben gezeigt, daß Photofrin II (Dihämatoporphyrinester und -ether) an Kulturen glatter Muskelzellen aus atherosklerotischen Plaques des Menschen eine verminderte Proliferationsaktivität und Zellvitalität mit und ohne Photoaktivierung bewirkt. Um die Wirkung von Photofrin II auf Endothelzellen des Menschen zu bestimmen, wurden subkultivierte Endothelzellen aus der Vena saphena magna in Proliferations- und Vitalitätstests untersucht sowie die Zellmorphologie nach Photofrin II-Einwirkung begutachtet. Eine Inkubation mit Photofrin II ohne Photoaktivierung über drei, sechs und neun Tage bewirkte erst ab einer Konzentration von 5 μg/ml eine eindeutige Proliferationshemmung. Bei dieser Konzentration von 5 μg/ml führte die photodynamische Reaktion zu lichtdosisabhängigen morphologischen Veränderungen und zum Absterben der Zellen; bei 1 μg/ml nahm die Zahl der vitalen Zellen innerhalb von 24 Stunden nicht wesentlich ab. Im Vergleich hierzu reagieren glatte Muskelzellen aus atherosklerotischen Plaques empfindlicher auf eine photodynamische Behandlung. Nach diesen in-vitro-Ergebnissen erscheint ein photodynamisches Therapiekonzept der Atherosklerose aussichtsreich.
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© 1992 Springer Fachmedien Wiesbaden
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Coppenrath, E., Ischinger, T., Dartsch, P.C., Betz, E. (1992). Effect of Photofrin II on human endothelial cells in culture. In: Heinle, H., Schulte, H., Schaefer, H.E. (eds) Arteriosklerotische Gefäßerkrankungen. Vieweg+Teubner Verlag, Wiesbaden. https://doi.org/10.1007/978-3-663-19646-4_49
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DOI: https://doi.org/10.1007/978-3-663-19646-4_49
Publisher Name: Vieweg+Teubner Verlag, Wiesbaden
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