Abstract
The photoacoustic spectra of light- and dark-adapted bacteriorhodopsin in aqueous and mixed water-glycerol (1:2)-suspensions are studied in the temperature range of 90–300 K. Photochemical transients are observed and interpreted in terms of the known photocycles of trans and 13-cis bacteriorhodopsin. Differences of the spectra with respect to those obtained by low temperature optical absorption spectroscopy of glassy glycerol-water suspensions are discussed.
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© 1984 Springer Fachmedien Wiesbaden
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Bechthold, P.S., Kohl, KD., Sperling, W. (1984). The Photocycles of Bacteriorhodopsin in Buffered Aqueous Suspension Studied at Low Temperatures by Means of Photoacoustic Spectroscopy. In: Lüscher, E., Korpiun, P., Coufal, HJ., Tilgner, R. (eds) Photoacoustic Effect Principles and Applications. Vieweg+Teubner Verlag, Wiesbaden. https://doi.org/10.1007/978-3-663-06820-4_23
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DOI: https://doi.org/10.1007/978-3-663-06820-4_23
Publisher Name: Vieweg+Teubner Verlag, Wiesbaden
Print ISBN: 978-3-528-08573-5
Online ISBN: 978-3-663-06820-4
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