Zusammenfassung
In 108 Serumproben wurden in 4 verschiedenen klinischen Laboratorien Serumkonzentrationen von Theophyllin bestimmt. Die kommerziell erhältlichen RIA-Kits GAMMA-DABR (Clinical Assays) und RIA-MATR (Byk-Mallinckrodt) und der Enzymimmunoassay EMIT-SYVAR (Merck) wurden benützt. Die Serumproben wurden gewonnen durch Blutentnahmen bei 12 gesunden männlichen Probanden nach Applikation von 3 verschiedenen Einzeldosen von 3 unterschiedlichen Theophyllin-Galeniken: 15, 60, 120 Minuten nach Gabe von 360 mg EuphyllinR i.v., 60, 120, 360 Minuten nach Gabe von 350 mg EuphyllinR retard Drg. oder 350 mg EuphyllinR Tbl. per os. Bei jeder Bestimmung ergab die Analyse von Referenzserumproben eine Variation innerhalb der Bestimmung von maximal 10 % oder zumeist weniger. Infolgedessen schien eine zuverlässige Analyse bei jeder Bestimmung vorhanden gewesen zu sein. Jedoch deckte die statistische Analyse signifikante Unterschiede zwischen den einzelnen Bestimmungen auf, die entweder auf Unterschiede des Laboratoriums oder der Methode zurückzuführen sind. Alle Bestimmungen zeigen in Übereinstimmung auf, daß subtherapeutische Serumkonzentrationen nach Gabe von üblichen Dosen EuphyllinR zu messen waren.
Summary
Serum concentrations of theophylline were measured in 108 serum samples in four different clinical laboratories. The commercially available RIA kits GAMMA-DABR (Clinical Assays) and RIA-MATR (Byk-Mallinckrodt) and the enzyme immunoassay EMIT-SYVAR (Merck) were used. Serum samples were collected from 12 healthy volunteers after administration of 3 different single doses of various theophylline formulations: 15, 60, 120 min after 360 mg EuphyllinR intravenouslz, 60, 120, 360 min after 350 mg EuphyllinR retard Drg. orally or 350 mg EuphyllinR Tbl. In all tests repeated analyses of reference standards indicated an intraassay variation of 10 % or less and the results indicate that the analyses are reliable. Statistical analysis, however, indicated significant differences between determinations which could be due to either laboratory or methodological differences. All determinations made showed that subtherapeutic serum concentrations of Theophylline were attained after administration of commonly used doses of EuphyllinR.
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Literature
Jenne, J. W., Wyze, E., Rood, F. S., MacDonald, F. M.: Pharmacokinetics of Theophylline. Application to adjustment of the clinical dose of Aminophylline. Clin. Pharmacol. Ther. 13, 349–360 (1972).
Mitenko, P. A., Ogilvie, R. I.: Pharmacokinetics of intravenous Theophylline. Clin. Pharmacol. Ther. 14, 509–513 (1973).
Piafsky, K. M., Ogilvie, R. I.: Dosage of Theophylline in Bronchial Asthma. New. Engl. J. Med. 292, 1218–1222 (1975).
Weinberger, M. W., Bronsky, E. A.: Evaluation of oral bronchodilator therapy in asthmatic children. J. Pediat. 84, 421–427 (1974).
Weinberger, M.: Theophylline for treatment of asthma. J. Pediat. 92, 1–7 (1978).
Jacobs, M. H., Senior, R. M., Kessler, G.: Clinical experience with Theophylline. Relationships between dosage, serum concentration and toxicity. J. Amer. med. Ass. 235, 1983–1986 (1976).
Shannon, D. C., Gotay, F., Stein, I. M., Rogers, M. C., Todres, I. D., Moylan, F. M. B.: Prevention of apnea and bradycardia in low-birthweight infants. Pediatrics 55, 589–594 (1975).
Hendeles, K., Weinberger, M., Johnson, G.: Monitoring serum Theophylline levels. Clin. Pharmacokin. 3, 294–312 (1978).
Weinberger, M., Matthay, R. A., Ginchansky, E. J., Chidsey, C. A., Petty, T. L.: Intravenous aminophylline dosage. Use of serum Theophylline measurement for guidance. J. Amer. med. Ass. 235, 2110–2113 (1976).
Schack, J. A., Waxler, S. H.: An ultraviolet spectrophotometric method for determination of Theophylline and Theobromine in blood and tissues. J. Pharmac. 97, 283–291 (1949).
Dusci, L. J., Hackett, L. P., McDonald, I. A.: Gas-liquid chromatographic determination of Theophylline in human plasma. J. Chromatogr. 104, 147–150 (1975).
Johnson, G. F., Dechtiaruk, W. A., Solomon, H. M.: Gaschromatographic determination of Theophylline in human serum and saliva. Clin. Chem. 21, 144–147 (1975).
Thomson, R. D., Nagasava, H. T., Jenne, J. W.: Determination of Theophylline and its metbolites in human urine and serum by high pressure liquid chromatography. J. Lab. Clin. Med. 84, 584–590 (1974).
Franconi, L. C., Hawk, G. L., Sandmann, B. J., Haney, W. G.: Determination of Theophylline in plasma ultrafiltrate by reversed phase high pressure liquid chromatography. Anal. Chem. 48, 372–375 (1976).
Orcutt, J. J., Kozak, P. P., Gilman, S. A., Cummins, L. H.: Microscale method for Theophylline in body fluids by reversed phase, high pressure liquid chromatography. Clin. Chem. 23, 599–601 (1977).
Neese, A. L., Soyka, L. F.: Development of a radioimmunoassay for Theophylline. Clin. Pharmacol. Ther. 21, 633–641 (1977).
Gushaw, J. B., Hu, M. W., Singh, P., Miller, J. G., Schneider, R. S.: Homogenous enzyme immnoassay for Theophylline in serum. Clin. Chem. 23, 1144 (1977).
Zech, K., Borner, K., von Stetlen, O.: Monitoring of serum or saliva Theophylline concentrations by a new radioimmunoassay. Fresenius Z. Anal. Chem. 301, 114 (1980).
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© 1982 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH, Braunschweig
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Rameis, H., Kaik, G., Endler, A.T., Nolte, D. (1982). Multicentre Comparative Study of Theophylline Concentration Estimations in Serum: (4 RIAs and 1 EMIT-Assay). In: Rietbrock, N., Woodcock, B.G., Staib, A.H. (eds) Theophylline and other Methylxanthines / Theophyllin und andere Methylxanthine. Methods in Clinical Pharmacology, vol 3. Vieweg+Teubner Verlag, Wiesbaden. https://doi.org/10.1007/978-3-663-05268-5_35
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DOI: https://doi.org/10.1007/978-3-663-05268-5_35
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