Abstract
Conjugated polymer dots emerge as attractive molecular imaging nanoprobes in living animals because of their excellent optical properties including bright fluorescence intensity, excellent photostability, high emission rates, and low intrinsic cytotoxicity. In this chapter we focused on the preparation of near-infrared (NIR)-emitting polymer dots by nano-precipitation method (matrix-encapsulation method), miniemulsion method, and in situ colloidal Knoevenagel polymerization methods and their applications for in vivo NIR fluorescence imaging, including imaging of lymphatic basins, tumors, zebrafish, and oxygen.
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Abbreviations
- AIE:
-
Aggregation-induced-emission
- BODIPY:
-
Phthalocyanine- and boron dipyrrin
- BRET:
-
Bioluminescence resonance energy transfer
- BSA:
-
Bovine serum albumin
- BTE:
-
Bisthienylethene derivative, 1,2-bis(2,4-dimethyl-5-phenyl-3-thienyl)-3,3,4,4,5,5-hexafluoro-1-cyclopentene
- BTTP:
-
5,7-bis(5-bromo-2-thienyl)-2,3-dimethylthieno[3,4-b]pyrazine
- BTTPF:
-
BTTP copolymized with 2,7-bis(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-9,9-bis[30-(dimethylamino)propyl] fluorine
- C7-FA:
-
Homemade heptylamine modified folate
- CN-PPVs:
-
Cyanosubstituted derivatives of poly(p-phenylenevinylene)
- CPDP-FA NPs:
-
CP-loaded DSPE-PEG-folic acid nanoparticles
- cRGD:
-
Cyclic RGD peptides
- cvCP:
-
Anovinylene-backboned conjugated polymers
- cvPDs:
-
Cyanovinylene-backboned polymer dots
- CZ:
-
3,6-dibromo-9-(2-butyloctyl)-9H–carbazole
- Dox:
-
Doxorubicin
- DPPF:
-
poly{3-(5-(9-hexyl-9-octyl-9H-uoren-2-yl)thiophen-2-yl)-2,5-bis(2-hexyldecyl)-6-(thiophen-2-yl)pyrrolo[3,4-c]pyrrole-1,4(2H,5H)-dione}
- DSPE:
-
2-distearoyl-sn-glycero-3-phosphoethanolamine
- DSPE-PEG2000:
-
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000]
- DSPE-PEG-NH2:
-
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000]
- DTT:
-
2,6-dibromo-4,4-dihexyl-4H-cyclopenta[1,2-b:5,4-b’]dithiophene
- EPR:
-
Enhanced permeability and retention
- FRET:
-
Fluorescence resonance energy transfer
- MEH-PPV:
-
poly[2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylenevinylene]
- NanoDRONE:
-
Dual-color SPN-based NIR nanoprobe for the detection of RONS
- NIR775:
-
Silicon 2, 3-naphthalocyanine bis(trihexylsilyloxide)
- PCFDP:
-
[9,9′-dihexyl-2,7-bis(1-cyanovinylene)fluorenylene-alt-co-2,5-bis(N,N′-diphenylamino)-1,4-phenylene]
- PCPDTBT:
-
poly[2,6-(4,4-bis-(2-ethylhexyl)-4H–cyclopenta[2,1-b;3,4-b0]dithiophene)-alt-4,7(2,1,3-benzothiadiazole)]
- PD-1:
-
PS-PEG-COOH modified MEH-PPV polymer dots
- PD-2:
-
PSMA modified MEH-PPV polymer dots
- PD-3:
-
PS-PEG-COOH modified and Luc-8 conjugated MEH-PPV polymer dots
- PD-4:
-
PS-PEG-COOH modified and RGD conjugated MEH-PPV polymer dots
- PD-5:
-
PS-PEG-COOH modified, RGD and Luc-8 conjugated MEH-PPV polymer dots
- PDTPDTT:
-
poly[5-octyl-1-(5-(4,4,9,9-tetrakis(4-hexylphenyl)-4,9-dihydro-s-indaceno[1,2-b:5,6-b0]dithiophen-2-yl)thiophen-2-yl)-3-(thiophen-2-yl)-4H–thieno[3,4-c]pyrrole-4, 6(5H)-dione]
- PEG-NH2:
-
Methoxypolyethylene glycol amine 2000
- PFBT:
-
poly(9,9-dioctylfluorene-alt-benzothiadiazole) average Mn 10,000–20,000
- PFBTDBT10:
-
poly[(9,9-dihexylfluorene)-co-2,1,3-benzothiadiazole-co-4,7-di(thiophen-2-yl)-2,1,3-benzothiadiazole]
- PFVBT:
-
poly[9,9-bis(6′-(N,N-dimethylamino)hexyl)fluorenyldivinylenealt-4,7-(2,1,3,-benzothiadiazole)]
- PIDT-DBT:
-
poly{[4,4,9,9-tetrakis(4-(octyloxy)phenyl-4,9-dihydro-s-indaceno[1,2-b:5,6-b′]dithiophene)]-alt-co-[4,7-di(thiophen-2-yl)-2,1,3-benzothiadiazole]}
- PLGA-PEG-FOL:
-
poly(lactic-co-glycolic acid)-poly(ethylene glycol)-folate
- PSBTBT:
-
poly[2,6-(4,40-bis(2-ethylhexyl)dithieno[3,2-b:20,30-d]silole)-alt-4,7(2,1,3-benzothiadiazole)]
- PSMA:
-
poly(styrene-alt-maleic anhydride)
- PS-PEG-COOH:
-
Polystyrene-graft-ethyleneoxide functionalized with carboxyl groups
- PtTFPPBr2:
-
Platinum(II) meso-bis(pentafluorophenyl)bis-(4-bromophenyl)porphyrin
- RONS:
-
Reactive oxygen and nitrogen species
- ROS:
-
Reactive oxygen species
- SPNs:
-
Semiconducting polymer nanoparticles
- TC6FQ:
-
5,8-Bis(5-bromo-4-hexylthiophen-2-yl)-6,7-difluoro-2,3-bis-(3-(hexyloxy)phenyl)quinoxaline
- TEOS:
-
Tetraethyl orthosilicate
- TFPP:
-
meso-bis(pentafluorophenyl)bis-(4-bromophenyl)porphyrin
- TPE-TPA-DCM:
-
2-(2,6-Bis((E)-4-(phenyl(4′-(1,2,2-triphenylvinyl)-[1,1′-biphenyl]-4-yl)amino)styryl)-4H–pyran-4-ylidene)malononitrile
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Guo, Y., Xiong, L. (2018). In Vivo Near-Infrared Fluorescence Imaging Based on Polymer Dots. In: Kumar, C. (eds) Nanotechnology Characterization Tools for Biosensing and Medical Diagnosis. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-56333-5_13
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DOI: https://doi.org/10.1007/978-3-662-56333-5_13
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