Collection, preservation and culturing

  • Pavel Škaloud
  • Fabio Rindi
  • Christian Boedeker
  • Frederik Leliaert
Chapter
Part of the Süßwasserflora von Mitteleuropa book series (414, volume 13)

Zusammenfassung

Macroscopic freshwater ulvophytes, including Cladophora and Ulva species, are easily observable and collectable. For these species, thalli can be preserved as dried herbarium specimens, or as wet specimens in 5 % formalin solution or 95 % ethanol. For molecular characterization, clean portions of the thallus (e. g. apical tips or young thallus parts) can be dried in silica-gel, or preserved in 95 % ethanol. Unialgal cultures of these larger ulvophytes can be prepared by incubating clean, young apical portions of the thallus into freshwater culture medium, or by isolation of zoospores.

Copyright information

© Springer-Verlag GmbH Germany 2018

Authors and Affiliations

  • Pavel Škaloud
    • 1
  • Fabio Rindi
    • 2
  • Christian Boedeker
    • 3
  • Frederik Leliaert
    • 4
  1. 1.Department of Botany, Faculty of ScienceCharles UniversityPraha 2Tschechische Republik
  2. 2.Dipartimento di Scienze della Vita e dell’AmbienteUniversità Politecnica delle MarcheAnconaItalien
  3. 3.School of Biological SciencesVictoria University of WellingtonWellingtonNeuseeland
  4. 4.Phycology Research Group, Biology DepartmentGhent UniversityGentBelgien

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