Abstract
Porous protein structures render biomaterials similar to their natural counterparts, extracellular matrices (ECMs), regarding both structure and material. Proteins in fibrous form have attracted considerable attention for fabrication of porous structures, as ECMs are composed of nanoscale protein fibers oriented randomly in three dimensions. Pores or voids created by random arrangements of the fibers provide spaces for cells to grow and spread. Fibrous structures could further facilitate cell attachment and guide cellular development and signaling. As technical difficulties have been gradually tackled, developing fibrous proteinous structures as biomaterials are arousing more interests. Micro- and nanofibrous structures have been developed from animal proteins, e.g., collagen, fibroin, keratin, and plant proteins, e.g., zein, soyprotein, and wheat gluten, via wet spinning, electrospinning, phase separation and other approaches. However, proteins as biomaterials usually suffer from inferior water stability, fast degradation, and poor mechanical properties. To circumvent these problems, crosslinking approaches have been applied, or synthetic polymers have been incorporated to improve the performance properties of proteins in aqueous environments.
The original version of this chapter was revised.
An erratum to this chapter can be found at https://doi.org/10.1007/978-3-662-53804-3_14.
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References
Ito Y (1999) Surface micropatterning to regulate cell functions. Biomaterials 20:2333–2342
Ruoslahti E (1996) RGD and other recognition sequence for integrins. Annu Rev Cel Dev Biol 12:697–715
Reddy N, Yang Y (2007) Novel protein fibers from wheat gluten. Biomacromolecules 8:638–643
Zhou J, Cao C, Ma X, Lin J (2010) Electrospinning of silk fibroin and collagen for vascular tissue engineering. Int J Biol Macromol 47:514–519
Nam J, Huang Y, Agarwal S, Lannutti J (2007) Improved cellular infiltration in electrospun fiber via engineered porosity. Tissue Eng 13:2249–2257
Park SY, Ki CS, Park YH, Jung HM, Woo KM, Kim HJ (2010) Electrospun silk fibroin scaffolds with macropores for bone regeneration: an in vitro and in vivo study. Tissue Eng A 16:1271–1279
Cai S, Xu H, Jiang Q, Yang Y (2013) Novel 3D electrospun scaffolds with fibers oriented randomly and evenly in three dimensions to closely mimic the unique architectures of extracellular matrices in soft tissues: fabrication and mechanism study. Langmuir 29:2311–2318
Wei G, Ma PX (2006) Macroporous and nanofibrous polymer scaffolds and polymer/bone-like apatite composite scaffolds generated by sugar spheres. J Biomed Mater Res 78A:306–315
Wang YC, Lin MC, Wang DM, Hsieh HJ (2003) Fabrication of a novel porous PGA-chitosan hybrid matrix for tissue engineering. Biomaterials 24:1047–1057
Ma PX, Zhang R (1999) Synthetic nano-scale fibrous extracellular matrix. J Biomed Mater Res 46:60–72
Matthews JA, Wnek GE, Simpson DG, Bowlin GL (2002) Electrospinning of collagen nanofibers. Biomacromolecules 3:232–238
Shields KJ, Beckman MJ, Bowlin GL, Wayne JS (2004) Mechanical properties and cellular proliferation of electrospun collagen type II. Tissue Eng 10:1510–1517
Huang L, Nagapudi K, Apkarian RP, Chaikof EL (2001) Engineered collagen–PEO nanofibers and fabrics. J Biomater Sci Polym Ed 12:979–993
Dong B, Arnoult O, Smith ME, Wnek GE (2009) Electrospinning of collagen nanofiber scaffolds from benign solvents. Macromol Rapid Commun 30:539–542
Jiang Q, Reddy N, Zhang S, Roscioli N, Yang Y (2013) Water-stable electrospun collagen fibers from a non-toxic solvent and crosslinking system. J Biomed Mater Res A 101A:1237–1247
Reddy N, Reddy R, Jiang Q (2015) Crosslinking biopolymers for biomedical applications. Trends Biotechnol 33(6):362–369
Xu H, Yang Y (2014) Controlled de-cross-linking and disentanglement of feather keratin for fiber preparation via a novel process. ACS Sustain Chem Eng 2(6):1404–1410
Xu H, Cai S, Xu L, Yang Y (2014) Water-stable three-dimensional ultrafine fibrous scaffolds from keratin for cartilage tissue engineering. Langmuir 30(28):8461–8470
Matthews JA, Boland ED, Wnek GE, Simpson DG, Bowlin GL (2003) Electrospinning of collagen type II: a feasibility study. J Bioact Compat Polym 18:125–134
Buttafoco L, Kolkman NG, Engbers-Buijtenhuijs P, Poot AA, Dijkstra PJ, Vermes I, Feijen J (2006) Electrospinning of collagen and elastin for tissue engineering applications. Biomaterials 27:724–734
Tillman BW, Yazdani SK, Lee SJ, Geary RL, Atala A, Yoo JJ (2009) The in vivo stability of electrospun polycaprolactone-collagen scaffolds in vascular reconstruction. Biomaterials 30:583–588
Meyer M, Baltzer H, Schwikal K (2010) Collagen fibres by thermoplastic and wet spinning. Mater Sci Eng C 30:1266–1271
Chen ZG, Wang PW, Wei B, Mo XM, Cui FZ (2010) Electrospun collagen–chitosan nanofiber: a biomimetic extracellular matrix for endothelial cell and smooth muscle cell. Acta Biomater 6:372–382
Rnjak-Kovacina J, Wise SG, Li Z, Maitz PKM, Young CJ, Wang Y, Weiss AS (2012) Electrospun synthetic human elastin: collagen composite scaffolds for dermal tissue engineering. Acta Biomater 8:3714–3722
Hosseinkhani H, Tabata Y (2003) In vitro gene expression by cationized derivatives of an artificial protein with repeated RGD sequences, Pronectin®. J Control Release 86:169–182
Van Vlierberghe S, Vanderleyden E, Dubruel P, De Vos F, Schacht E (2009) Affinity study of novel gelatin cell carriers for fibronectin. Macromol Biosci 9:1105–1115
Van Vlierberghe S, Dubruel P, Schacht E (2011) Biopolymer-based hydrogels as scaffolds for tissue engineering applications: a review. Biomacromolecules 12:1387–1408
Lei B, Shin KH, Noh DY, Jo IH, Koh YH, Choi WY, Kim HE (2012) Nanofibrous gelatin-silica hybrid scaffolds mimicking the native extracellular matrix (ECM) using thermally induced phase separation. J Mater Chem 22:14133–14140
Takagi J (2004) Structural basis for ligand recognition by RGD (Arg-Gly-Asp)-dependent integrins. Biochem Soc Trans 32:403–406
Rujitanaroj PO, Pimpha N, Supaphol P (2008) Wound-dressing materials with antibacterial activity from electrospun gelatin fiber mats containing silver nanoparticles. Polymer 49:4723–4732
Sisson K, Zhang C, Farach-Carson MC, Chase DB, Rabolt JF (2010) Fiber diameters control osteoblastic cell migration and differentiation in electrospun gelatin. J Biomed Mater Res A 94A:1312–1320
Liu X, Ma PX (2009) Phase separation, pore structure, and properties of nanofibrous gelatin scaffolds. Biomaterials 30:4094–4103
Li M, Mondrinos MJ, Gandhi MR, Ko FK, Weiss AS, Lelkes PI (2005) Electrospun protein fibers as matrices for tissue engineering. Biomaterials 26:5999–6008
Song JH, Kim HE, Kim HW (2008) Production of electrospun gelatin nanofiber by water-based co-solvent approach. J Mater Sci Mater Med 19:95–102
Panzavolta S, Gioffrè M, Focarete ML, Gualandi C, Foroni L, Bigi A (2011) Electrospun gelatin nanofibers: optimization of genipin cross-linking to preserve fiber morphology after exposure to water. Acta Biomater 7:1702–1709
Chen HC, Jao WC, Yang MC (2009) Characterization of gelatin nanofibers electrospun using ethanol/formic acid/water as a solvent. Polym Adv Technol 20:98–103
Aduba DC Jr, Hammer JA, Yuan Q, Andrew Yeudall W, Bowlin GL, Yang H (2013) Semi-interpenetrating network (sIPN) gelatin nanofiber scaffolds for oral mucosal drug delivery. Acta Biomater 9:6576–6584
Huang CH, Chi CY, Chen YS, Chen KY, Chen PL, Yao CH (2012) Evaluation of proanthocyanidin-crosslinked electrospun gelatin nanofibers for drug delivering system. Mater Sci Eng C 32:2476–2483
Altman GH, Diaz F, Jakuba C, Calabro T, Horan RL, Chen J, Lu H, Richmond J, Kaplan DL (2003) Silk-based biomaterials. Biomaterials 24:401–416
Jin HJ, Kaplan DL (2003) Mechanism of silk processing in insects and spiders. Nature 424:1057–1061
Jiang C, Wang X, Gunawidjaja R, Lin YH, Gupta MK, Kaplan DL, Naik RR, Tsukruk VV (2007) Mechanical properties of robust ultrathin silk fibroin films. Adv Funct Mater 17:2229–2237
Liu H, Fan H, Wang Y, Toh SL, Goh JCH (2008) The interaction between a combined knitted silk scaffold and microporous silk sponge with human mesenchymal stem cells for ligament tissue engineering. Biomaterials 29:662–674
Shangkai C, Naohide T, Koji Y, Yasuji H, Masaaki N, Tomohiro T, Yasushi T (2007) Transplantation of allogeneic chondrocytes cultured in fibroin sponge and stirring chamber to promote cartilage regeneration. Tissue Eng 13:483–492
Jin HJ, Chen J, Karageorgiou V, Altman GH, Kaplan DL (2004) Human bone marrow stromal cell responses on electrospun silk fibroin mats. Biomaterials 25:1039–1047
Kinahan ME, Filippidi E, Köster S, Hu X, Evans HM, Pfohl T, Kaplan DL, Wong J (2011) Tunable silk: using microfluidics to fabricate silk fibers with controllable properties. Biomacromolecules 12:1504–1511
Yan J, Zhou G, Knight DP, Shao Z, Chen X (2009) Wet-spinning of regenerated silk fiber from aqueous silk fibroin solution: discussion of spinning parameters. Biomacromolecules 11:1–5
Kim JH, Park CH, Lee OJ, Lee JM, Kim JW, Park YH, Ki CS (2012) Preparation and in vivo degradation of controlled biodegradability of electrospun silk fibroin nanofiber mats. J Biomed Mater Res A 100A:3287–3295
Cristino S, Grassi F, Toneguzzi S, Piacentini A, Grigolo B, Santi S, Riccio M, Tognana E, Facchini A, Lisignoli G (2005) Analysis of mesenchymal stem cells grown on a three-dimensional HYAFF 11®-based prototype ligament scaffold. J Biomed Mater Res A 73A:275–283
Wang Y, Kim UJ, Blasioli DJ, Kim HJ, Kaplan DL (2005) In vitro cartilage tissue engineering with 3D porous aqueous-derived silk scaffolds and mesenchymal stem cells. Biomaterials 26:7082–7094
Min BM, Lee G, Kim SH, Nam YS, Lee TS, Park WH (2004) Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro. Biomaterials 25:1289–1297
Lovett ML, Cannizzaro CM, Vunjak-Novakovic G, Kaplan DL (2008) Gel spinning of silk tubes for tissue engineering. Biomaterials 29:4650–4657
Liu H, Li X, Zhou G, Fan H, Fan Y (2011) Electrospun sulfated silk fibroin nanofibrous scaffolds for vascular tissue engineering. Biomaterials 32:3784–3793
Pan H, Zhang Y, Hang Y, Shao H, Hu X, Xu Y, Feng C (2012) Significantly reinforced composite fibers electrospun from silk fibroin/carbon nanotube aqueous solutions. Biomacromolecules 13:2859–2867
Aznar-Cervantes S, Roca MI, Martinez JG, Meseguer-Olmo L, Cenis JL, Moraleda JM, Otero TF (2012) Fabrication of conductive electrospun silk fibroin scaffolds by coating with polypyrrole for biomedical applications. Bioelectrochemistry 85:36–43
Kishimoto Y, Ito F, Usami H, Togawa E, Tsukada M, Morikawa H, Yamanaka S (2013) Nanocomposite of silk fibroin nanofiber and montmorillonite: fabrication and morphology. Int J Biol Macromol 57:124–128
Reddy N, Yang Y (2011) Potential of plant proteins for medical applications. Trends Biotechnol 29(10):490–498
Li Y, Lim LT, Kakuda Y (2009) Electrospun zein fibers as carriers to stabilize (−)‐epigallocatechin gallate. J Food Sci 74(3):C233–C240
Jiang YN, Mo HY, Yu DG (2012) Electrospun drug-loaded core–sheath PVP/zein nanofibers for biphasic drug release. Int J Pharm 438:232–239
Selling GW, Woods KK, Sessa D, Biswas A (2008) Electrospun zein fibers using glutaraldehyde as the crosslinking reagent: effect of time and temperature. Macromol Chem Phys 209:1003–1011
Jiang Q, Reddy N, Yang Y (2010) Cytocompatible cross-linking of electrospun zein fibers for the development of water-stable tissue engineering scaffolds. Acta Biomater 6(10):4042–4051
Karthikeyan K, Guhathakarta S, Rajaram R, Korrapati PS (2012) Electrospun zein/eudragit nanofibers based dual drug delivery system for the simultaneous delivery of aceclofenac and pantoprazole. Int J Pharm 438:117–122
Lin J, Li C, Zhao Y, Hu J, Zhang LM (2012) Co-electrospun nanofibrous membranes of collagen and zein for wound healing. ACS Appl Mater Interfaces 4:1050–1057
Brahatheeswaran D, Mathew A, Aswathy RG, Nagaoka Y, Venugopal K, Yoshida Y, Maekawa T, Sakthikumar D (2012) Hybrid fluorescent curcumin loaded zein electrospun nanofibrous scaffold for biomedical applications. Biomed Mater 7:045001
Huang W, Zou T, Li S, Jing J, Xia X, Liu X (2013) Drug-loaded zein nanofibers prepared using a modified coaxial electrospinning process. AAPS PharmSciTech 14:675–681
Yang JM, Zha LS, Yu DG, Liu J (2013) Coaxial electrospinning with acetic acid for preparing ferulic acid/zein composite fibers with improved drug release profiles. Colloids Surf B: Biointerfaces 102:737–743
Woerdeman DL, Ye P, Shenoy S, Parnas RS, Wnek GE, Trofimova O (2005) Electrospun fibers from wheat protein: investigation of the interplay between molecular structure and the fluid dynamics of the electrospinning process. Biomacromolecules 6:707–712
Reddy N, Yang Y (2008) Self-crosslinked gliadin fibers with high strength and water stability for potential medical applications. J Mater Sci Mater Med 19:2055–2061
Reddy N, Yang Y (2009) Soyprotein fibers with high strength and water stability for potential medical applications. Biotechnol Progr 25:1796–1802
Woerdeman DL, Shenoy S, Breger D (2007) Effects of hydroxyl groups versus physical entanglements on the electrospinning behavior of wheat protein. J Biobased Mater Bioenergy 1:31–36
Xu W, Yang Y (2010) Drug loading onto and release from wheat gluten fibers. J Appl Polym Sci 116:708–717
Xu W, Yang Y (2009) Drug sorption onto and release from soy protein fibers. J Mater Sci Mater Med 20:2477–2486
Vega-Lugo AC, Lim LT (2009) Controlled release of allyl isothiocyanate using soy protein and poly(lactic acid) electrospun fibers. Food Res Int 42:933–940
Sinha-Ray S, Zhang Y, Yarin AL, Davis SC, Pourdeyhimi B (2011) Solution blowing of soy protein fibers. Biomacromolecules 12:2357–2363
Xu X, Jiang L, Zhou Z, Wu X, Wang Y (2012) Preparation and properties of electrospun soy protein isolate/polyethylene oxide nanofiber membranes. ACS Appl Mater Interfaces 4:4331–4337
Cho D, Netravali AN, Joo YL (2012) Mechanical properties and biodegradability of electrospun soy protein Isolate/PVA hybrid nanofibers. Polym Degrad Stab 97:747–754
Xu H, Cai S, Sellers A, Yang Y (2014) Intrinsically water-stable electrospun three-dimensional ultrafine fibrous soy protein scaffolds for soft tissue engineering using adipose derived mesenchymal stem cells. RSC Adv 4(30):15451–15457
Dong J, Asandei AD, Parnas RS (2010) Aqueous electrospinning of wheat gluten fibers with thiolated additives. Polymer 51:3164–3172
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Xu, H., Yang, Y. (2017). Porous Structures from Fibrous Proteins for Biomedical Applications. In: Yang, Y., Yu, J., Xu, H., Sun, B. (eds) Porous lightweight composites reinforced with fibrous structures. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-53804-3_7
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DOI: https://doi.org/10.1007/978-3-662-53804-3_7
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