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Solid-Phase Microextraction in Binding Studies

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Solid Phase Microextraction

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Abstract

This chapter briefly discusses how solid-phase microextraction (SPME) can be successfully implemented to accurately measure ligand–receptor binding constants, % plasma protein binding, blood-to-plasma distribution ratios, and free versus total analyte concentrations. The main practical considerations and assumptions to successfully apply SPME to binding studies are discussed in detail, including the effect of significant versus negligible depletion, extraction time, pH, and fiber fouling. The binding results obtained with SPME are compared to literature values and to other techniques used for binding determinations, and the advantages and disadvantages of different methods are clearly discussed. Recent applications of SPME in binding studies are highlighted, with the primary focus on binding studies in biological fluids and tissues since this application area is where SPME has been most extensively applied. Highlighted examples show that SPME has been successfully applied for ligands with both low and high binding affinities, for ligands with different affinities to multiple sites on a given receptor, to study differences in inter-species binding as well as partitioning coefficients for various tissue types. The importance of studying inter-individual variability of free concentrations in future studies is highlighted and can contribute to the development of personalized medicine.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry and PERFORM Centre, Concordia University, Montreal, Canada

    Dajana Vuckovic

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  1. Dajana Vuckovic
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Correspondence to Dajana Vuckovic .

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Editors and Affiliations

  1. School of Chemistry, Sun Yat-sen University, Guangzhou, Guangdong, China

    Gangfeng Ouyang

  2. School of Environment, Jinan University, Guangzhou, Guangdong, China

    Ruifen Jiang

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Vuckovic, D. (2017). Solid-Phase Microextraction in Binding Studies. In: Ouyang, G., Jiang, R. (eds) Solid Phase Microextraction. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-53598-1_10

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