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The Sequencing: How it was Done and What it Produced

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The Tomato Genome

Part of the book series: Compendium of Plant Genomes ((CPG))

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Abstract

The tomato genome sequencing was part of a larger international project whose final aim was to develop a network of resources focusing on the biology of the plant and to address key questions about adaptation and diversification in the Solanaceae family. Solanum lycopersicum was chosen as a model system by virtue of the wealth of its genetic resources and was sequenced by the International SOL Consortium including 10 different countries. Initially the project started with a BAC-by-BAC strategy with the support of dense genetic and physical maps. With the advent of Next Generation Sequencing (NGS) techniques, strategies were revised to a primarily Whole Genome Shotgun (WGS) approach. The published genome is the result of the combination of the data obtained from both methodologies and represents a golden standard among Solanaceae that will serve different aspects of basic and applied research.

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Acknowledgments

We are grateful to the Tomato Genome Consortium for producing the data used in this chapter; to Lukas Mueller and the Solanaceae Genomics Network for providing Figs. 6.1, 6.2 and 6.3; to Helene Berges for granting us the use of Fig. 6.5, to Jose Luis Goicoechea and Bruce Roe for Fig. 6.6; Tables 6.3 and 6.5. We also thank Helene Berges, Jose Luis Goicoechea, Bruce Roe, Stephane Rombauts, and Steve Stack for carefully revising the manuscript.

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Authors and Affiliations

  1. Italian National Agency for New Technologies, Energy and Sustainable Development (ENEA), Casaccia Research Center, Via Anguillarese 301, 00123, Rome, Italy

    Marco Pietrella & Giovanni Giuliano

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  1. Marco Pietrella
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  2. Giovanni Giuliano
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Correspondence to Giovanni Giuliano .

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Editors and Affiliations

  1. GAFL, INRA, Montfavet Cedex, France

    Mathilde Causse

  2. Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York, USA

    Jim Giovannoni

  3. INRA-INP Toulouse, Castanet Tolosan, France

    Mondher Bouzayen

  4. INRA-INP Toulouse, Castanet Tolosan, France

    Mohamed Zouine

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Pietrella, M., Giuliano, G. (2016). The Sequencing: How it was Done and What it Produced. In: Causse, M., Giovannoni, J., Bouzayen, M., Zouine, M. (eds) The Tomato Genome. Compendium of Plant Genomes. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-53389-5_6

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