Skip to main content
SpringerLink
Log in

Extraction of Proteins with ABS

  • Chapter
  • First Online:
Ionic-Liquid-Based Aqueous Biphasic Systems

Part of the book series: Green Chemistry and Sustainable Technology ((GCST))

Abstract

Over the past years, there has been an increasing trend in research on the extraction and purification of proteins using aqueous biphasic systems (ABS) formed by polymers, e.g., polyethylene glycol (PEG). In general, when dealing with protein purification processes, it is essential to maintain their native structure and functional stability. In this context, ABS, liquid-liquid systems where both phases are water-rich, provide a biocompatible medium for such attempts. More recently, it was shown that the versatility offered by ABS is further enhanced by the introduction of ionic liquids (ILs) as alternative phase-forming components. This chapter describes and highlights the current progress on the field of protein extraction and purification using IL-based ABS. The general approach for protein extraction using IL-based ABS and factors influencing the partitioning are discussed. In addition, the challenges to overcome the use of IL-based ABS for protein extraction are also presented.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Kiron V, Phromkunthong W, Huntley M, Archibald I, De Scheemaker G (2012) Marine microalgae from biorefinery as a potential feed protein source for Atlantic salmon, common carp and whiteleg shrimp. Aquac Nutr 18(5):521–531. doi:10.1111/j.1365-2095.2011.00923.x

    Article  CAS  Google Scholar 

  2. Schwenzfeier A, Wierenga PA, Gruppen H (2011) Isolation and characterization of soluble protein from the green microalgae Tetraselmis sp. Bioresour Technol 102(19):9121–9127. doi:http://dx.doi.org/10.1016/j.biortech.2011.07.046

    Google Scholar 

  3. Wijffels RH, Barbosa MJ (2010) An outlook on microalgal biofuels. Science 329(5993):796–799. doi:10.1126/science.1189003

    Article  CAS  Google Scholar 

  4. Labrou NE (2014) Protein downstream processing: design, development and application of high and low-resolution methods. Humana Press, Totowa

    Book  Google Scholar 

  5. Albertsson P-A (1958) Partition of proteins in liquid polymer-polymer two-phase systems. Nature 182(4637):709–711

    Article  CAS  Google Scholar 

  6. Rosa PAJ, Azevedo AM, Sommerfeld S, Bäcker W, Aires-Barros MR (2011) Aqueous two-phase extraction as a platform in the biomanufacturing industry: economical and environmental sustainability. Biotechnol Adv 29(6):559–567. doi:http://dx.doi.org/10.1016/j.biotechadv.2011.03.006

    Google Scholar 

  7. Rosa PAJ, Ferreira IF, Azevedo AM, Aires-Barros MR (2010) Aqueous two-phase systems: a viable platform in the manufacturing of biopharmaceuticals (extraction techniques). J Chromatogr A 1217(16):2296–2305. doi:http://dx.doi.org/10.1016/j.chroma.2009.11.034

    Google Scholar 

  8. Gutowski KE, Broker GA, Willauer HD, Huddleston JG, Swatloski RP, Holbrey JD, Rogers RD (2003) Controlling the aqueous miscibility of ionic liquids: aqueous biphasic systems of water-miscible ionic liquids and water-structuring salts for recycle, metathesis, and separations. J Am Chem Soc 125(22):6632–6633. doi:10.1021/ja0351802

    Article  CAS  Google Scholar 

  9. Yang Z (2012) Ionic liquids and proteins: academic and some practical interactions. In: Ionic liquids in biotransformations and organocatalysis. Wiley, pp 15–71. doi:10.1002/9781118158753.ch2

    Google Scholar 

  10. Freire MG, Pereira JFB, Francisco M, Rodríguez H, Rebelo LPN, Rogers RD, Coutinho JAP (2012) Insight into the interactions that control the phase behaviour of new aqueous biphasic systems composed of polyethylene glycol polymers and ionic liquids. Chem Eur J 18(6):1831–1839. doi:10.1002/chem.201101780

    Article  CAS  Google Scholar 

  11. Wu B, Zhang YM, Wang HP (2008) Aqueous biphasic systems of hydrophilic ionic liquids + sucrose for separation. J Chem Eng Data 53(4):983–985. doi:10.1021/je700729p

    Article  CAS  Google Scholar 

  12. Zhang J, Zhang Y, Chen Y, Zhang S (2007) Mutual coexistence curve measurement of aqueous biphasic systems composed of [bmim][BF4] and glycine, l-serine, and l-proline, respectively. J Chem Eng Data 52(6):2488–2490. doi:10.1021/je0601053

    Article  CAS  Google Scholar 

  13. Dreyer S, Kragl U (2008) Ionic liquids for aqueous two-phase extraction and stabilization of enzymes. Biotechnol Bioeng 99(6):1416–1424. doi:10.1002/bit.21720

    Article  CAS  Google Scholar 

  14. Freire MG, Cláudio AFM, Araújo JMM, Coutinho JAP, Marrucho IM, Lopes JNC, Rebelo LPN (2012) Aqueous biphasic systems: a boost brought about by using ionic liquids. Chem Soc Rev 41(14):4966–4995

    Google Scholar 

  15. Zhang Y, Zhang S, Chen Y, Zhang J (2007) Aqueous biphasic systems composed of ionic liquid and fructose (4th MTMS 4th international symposium on molecular thermodynamics and molecular simulation). Fluid Phase Equilib 257(2):173–176. doi:http://dx.doi.org/10.1016/j.fluid.2007.01.027

    Google Scholar 

  16. Ding X, Wang Y, Zeng Q, Chen J, Huang Y, Xu K (2014) Design of functional guanidinium ionic liquid aqueous two-phase systems for the efficient purification of protein. Analytica Chimica Acta 815(0):22–32. doi:http://dx.doi.org/10.1016/j.aca.2014.01.030

    Google Scholar 

  17. Zeng Q, Wang Y, Li N, Huang X, Ding X, Lin X, Huang S, Liu X (2013) Extraction of proteins with ionic liquid aqueous two-phase system based on guanidine ionic liquid. Talanta 116(0):409–416. doi:http://dx.doi.org/10.1016/j.talanta.2013.06.011

    Google Scholar 

  18. Dreyer S, Salim P, Kragl U (2009) Driving forces of protein partitioning in an ionic liquid-based aqueous two-phase system. Biochem Eng J 46(2):176–185

    Article  CAS  Google Scholar 

  19. Pei Y, Wang J, Wu K, Xuan X, Lu X (2009) Ionic liquid-based aqueous two-phase extraction of selected proteins. Sep Purif Technol 64(3):288–295

    Article  CAS  Google Scholar 

  20. Constantinescu D, Weingärtner H, Herrmann C (2007) Protein denaturation by ionic liquids and the Hofmeister series: a case study of aqueous solutions of ribonuclease A. Angew Chem Int Ed 46(46):8887–8889. doi:10.1002/anie.200702295

    Article  CAS  Google Scholar 

  21. Takekiyo T, Yamazaki K, Yamaguchi E, Abe H, Yoshimura Y (2012) High ionic liquid concentration-induced structural change of protein in aqueous solution: a case study of lysozyme. J Phys Chem B 116(36):11092–11097. doi:10.1021/jp3057064

    Article  CAS  Google Scholar 

  22. Zhang W-G, Wei D-Z, Yang X-P, Song Q-X (2006) Penicillin acylase catalysis in the presence of ionic liquids. Bioprocess Biosyst Eng 29(5–6):379–383. doi:10.1007/s00449-006-0085-9

    Article  CAS  Google Scholar 

  23. Nara SJ, Harjani JR, Salunkhe MM (2002) Lipase-catalysed transesterification in ionic liquids and organic solvents: a comparative study. Tetrahedron Letters 43(16):2979–2982. doi:http://dx.doi.org/10.1016/S0040-4039(02)00420-3

    Google Scholar 

  24. Li Z, Pei Y, Wang H, Fan J, Wang J (2010) Ionic liquid-based aqueous two-phase systems and their applications in green separation processes (bioCop – monitoring chemical contaminants in foods). TrAC Trends Anal Chem 29(11):1336–1346. doi:http://dx.doi.org/10.1016/j.trac.2010.07.014

    Google Scholar 

  25. Desai R, Streefland M, Wijffels RH, Eppink M (2014) Extraction and stability of selected proteins in ionic liquid based aqueous two phase systems. Green Chem 16:2670. doi:10.1039/c3gc42631a

    Article  CAS  Google Scholar 

  26. Baker GA, Heller WT (2009) Small-angle neutron scattering studies of model protein denaturation in aqueous solutions of the ionic liquid 1-butyl-3-methylimidazolium chloride (application of ionic liquids in chemical and environmental engineering). Chem Eng J 147(1):6–12. doi:http://dx.doi.org/10.1016/j.cej.2008.11.033

    Google Scholar 

  27. Weaver KD, Vrikkis RM, Van Vorst MP, Trullinger J, Vijayaraghavan R, Foureau DM, McKillop IH, MacFarlane DR, Krueger JK, Elliott GD (2012) Structure and function of proteins in hydrated choline dihydrogen phosphate ionic liquid. Phys Chem Chem Phys 14(2):790–801

    Article  CAS  Google Scholar 

  28. Fujita K, MacFarlane DR, Forsyth M, Yoshizawa-Fujita M, Murata K, Nakamura N, Ohno H (2007) Solubility and stability of cytochrome c in hydrated ionic liquids: effect of oxo acid residues and kosmotropicity. Biomacromolecules 8(7):2080–2086. doi:10.1021/bm070041o

    Article  CAS  Google Scholar 

  29. Fukaya Y, Hayashi K, Wada M, Ohno H (2008) Cellulose dissolution with polar ionic liquids under mild conditions: required factors for anions. Green Chem 10(1):44–46. doi:10.1039/b713289a

    Article  CAS  Google Scholar 

  30. Kilpeläinen I, Xie H, King A, Granstrom M, Heikkinen S, Argyropoulos DS (2007) Dissolution of wood in ionic liquids. J Agric Food Chem 55(22):9142–9148. doi:10.1021/jf071692e

    Article  Google Scholar 

  31. Debeljuh N, Barrow CJ, Byrne N (2011) The impact of ionic liquids on amyloid fibrilization of A[small beta]16-22: tuning the rate of fibrilization using a reverse Hofmeister strategy. Phys Chem Chem Phys 13(37):16534–16536. doi:10.1039/c1cp22256b

    Article  CAS  Google Scholar 

  32. Zhao H (2005) Effect of ions and other compatible solutes on enzyme activity, and its implication for biocatalysis using ionic liquids. J Mol Catal B Enzym 37(1–6):16–25. doi:10.1016/j.molcatb.2005.08.007

    Article  CAS  Google Scholar 

  33. Baldwin RL (1996) How Hofmeister ion interactions affect protein stability. Biophys J 71(4):2056–2063. doi:S0006-3495(96)79404-3 [pii] 10.1016/S0006-3495(96)79404-3

  34. Collins KD, Washabaugh MW (1985) The Hofmeister effect and the behaviour of water at interfaces. Q Rev Biophys 18(4):323–422

    Article  CAS  Google Scholar 

  35. Albertsson PÅ (1986) Partition of cell particles and macromolecules: separation and purification of biomolecules, cell organelles, membranes, and cells in aqueous polymer two-phase systems and their use in biochemical analysis and biotechnology. Wiley, New York [etc.]

    Google Scholar 

  36. Lin X, Wang Y, Zeng Q, Ding X, Chen J (2013) Extraction and separation of proteins by ionic liquid aqueous two-phase system. Analyst 138(21):6445–6453. doi:10.1039/c3an01301d

    Article  CAS  Google Scholar 

  37. Chen J, Wang Y, Zeng Q, Ding X, Huang Y (2014) Partition of proteins with extraction in aqueous two-phase system by hydroxyl ammonium-based ionic liquid. Anal Methods 6(12):4067–4076. doi:10.1039/c4ay00233d

    Article  CAS  Google Scholar 

  38. Dreyer SE (2008) Aqueous two phase extraction of proteins and enzymes using tetraalkylammonium-based ionic liquids. PhD thesis, University of Rostock

    Google Scholar 

  39. Cao Q, Quan L, He C, Li N, Li K, Liu F (2008) Partition of horseradish peroxidase with maintained activity in aqueous biphasic system based on ionic liquid. Talanta 77(1):160–165. doi:http://dx.doi.org/10.1016/j.talanta.2008.05.055

    Google Scholar 

  40. Du Z, Yu Y-L, Wang J-H (2007) Extraction of proteins from biological fluids by use of an ionic liquid/aqueous two-phase system. Chem Eur J 13(7):2130–2137. doi:10.1002/chem.200601234

    Article  CAS  Google Scholar 

  41. Pei YC, Liu L, Li ZY, Wang JJ, Wang HY (2010) Selective separation of protein and saccharides by ionic liquids aqueous two-phase systems. SCIENCE CHINA Chem 53(7):1554–1560. doi:10.1007/s11426-010-4025-9

    Article  CAS  Google Scholar 

  42. Pereira MM, Pedro SN, Quental MV, Lima AS, Coutinho JAP, Freire MG (2015) Enhanced extraction of bovine serum albumin with aqueous biphasic systems of phosphonium- and ammonium-based ionic liquids. J Biotechnol 206:17–25

    Article  CAS  Google Scholar 

  43. Ruiz-Angel MJ, Pino V, Carda-Broch S, Berthod A (2007) Solvent systems for countercurrent chromatography: an aqueous two phase liquid system based on a room temperature ionic liquid (4th international conference on countercurrent chromatography). J Chromatogr A 1151(1–2):65–73

    Google Scholar 

  44. Wu C, Wang J, Wang H, Pei Y, Li Z (2011) Effect of anionic structure on the phase formation and hydrophobicity of amino acid ionic liquids aqueous two-phase systems. J Chromatogr A 1218(48):8587–8593. doi:http://dx.doi.org/10.1016/j.chroma.2011.10.003

    Google Scholar 

  45. Wu C, Wang J, Li Z, Jing J, Wang H (2013) Relative hydrophobicity between the phases and partition of cytochrome-c in glycine ionic liquids aqueous two-phase systems. J Chromatogr A 1305(0):1–6. doi:http://dx.doi.org/10.1016/j.chroma.2013.06.066

    Google Scholar 

  46. Yan J-K, Ma H-L, Pei J-J, Wang Z-B, Wu J-Y (2014) Facile and effective separation of polysaccharides and proteins from Cordyceps sinensis mycelia by ionic liquid aqueous two-phase system. Sep Purif Technol. doi:http://dx.doi.org/10.1016/j.seppur.2014.03.020

    Google Scholar 

  47. Deive FJ, Rodríguez A, Rebelo LPN, Marrucho IM (2012) Extraction of Candida antarctica lipase A from aqueous solutions using imidazolium-based ionic liquids (ILSEPT2011 special issue). Sep Purif Technol 97(0):205–210. doi:http://dx.doi.org/10.1016/j.seppur.2011.12.013

    Google Scholar 

  48. Ventura SPM, Sousa SG, Freire MG, Serafim LS, Lima ÁS, Coutinho JAP (2011) Design of ionic liquids for lipase purification. J Chromatogr B 879(26):2679–2687. doi:http://dx.doi.org/10.1016/j.jchromb.2011.07.022

    Google Scholar 

  49. Deive FJ, Rodriguez A, Pereiro AB, Araujo JMM, Longo MA, Coelho MAZ, Lopes JNC, Esperanca JMSS, Rebelo LPN, Marrucho IM (2011) Ionic liquid-based aqueous biphasic system for lipase extraction. Green Chem 13(2):390–396. doi:10.1039/c0gc00075b

    Article  CAS  Google Scholar 

  50. Jiang B, Feng Z, Liu C, Xu Y, Li D, Ji G (2014) Extraction and purification of wheat-esterase using aqueous two-phase systems of ionic liquid and salt. J Food Sci Technol 1–8. doi:10.1007/s13197-014-1319-5

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bioprocess Engineering Department, Wageningen University, 16, 6700 AA, Wageningen, The Netherlands

    Rupali K. Desai, Mathieu Streefland, Rene H. Wijffels & Michel H. M. Eppink

Authors
  1. Rupali K. Desai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mathieu Streefland
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rene H. Wijffels
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Michel H. M. Eppink
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rupali K. Desai .

Editor information

Editors and Affiliations

  1. CICECO – Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Aveiro, Portugal

    Mara G. Freire

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Desai, R.K., Streefland, M., Wijffels, R.H., Eppink, M.H.M. (2016). Extraction of Proteins with ABS. In: Freire, M. (eds) Ionic-Liquid-Based Aqueous Biphasic Systems. Green Chemistry and Sustainable Technology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-52875-4_6

Download citation

Publish with us

Policies and ethics

Search

Navigation