The Use of Scanning Electron Microscopy (SEM) in Visualizing the Root Canal Biofilm
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Apical periodontitis is caused by microorganisms in planktonic or biofilm state present in the root canal system and/or attached to the outer apical root surface. Knowledge about the microorganisms and biofilm structure within and external to the root canal system is important in order to effectively treat apical periodontitis. Scanning electron microscopy (SEM) has been used to visualize and morphologically describe the presence of biofilm and microorganisms associated with teeth with apical periodontitis. This chapter provides a short outline of the applications of SEM in endodontics with the intention to describe the benefits and shortcomings of this microscopic technique.
KeywordsRoot Canal Environmental Scanning Electron Microscopy Dentinal Tubule Endodontic Treatment Apical Periodontitis
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