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Intracellular and Organic miRNA In Situ Detection

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MicroRNA Detection and Pathological Functions

Part of the book series: SpringerBriefs in Molecular Science ((BRIEFSMOLECULAR))

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Abstract

The detection methods of miRNA in intracellular or organisms fall into two broad categories: indirect detection and direct analysis. The indirect measurement of the expression levels of miRNAs in cells and tissues involves cells lysis and detection by qRT-PCR, northern blotting, or microarray hybridization. The direct analysis methods are a noninvasive manner for repetitively monitoring and obtaining real-time imaging of the intracellular miRNA by using imaging analysis or in situ hybridization (ISH). Technologies for direct detection of the temporal and spatial expression sequence of specific miRNA in cells or tissues are extremely important for elucidating miRNA biology. The progress of optical imaging techniques with multimodal reporter systems holds great promise for noninvasive and real-time imaging of molecular agent expression in living cell. Recent progress in nanotechnology and imaging detection techniques leads to multifunctional nanoprobe with specific-transfection, tracing, and regulation function in intracellular miRNA detection. ISH holds great promise for visualization of the spatial localization of RNA at the tissue, cellular, and even subcellular level.

Haifeng Dong, Xiangdan Meng and Wenhao Dai contributed together to this chapter.

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Authors and Affiliations

  1. Research Center for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing, China

    Xueji Zhang & Haifeng Dong

  2. Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, China

    Yaping Tian

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  1. Xueji Zhang
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  2. Haifeng Dong
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  3. Yaping Tian
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Correspondence to Xueji Zhang .

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Zhang, X., Dong, H., Tian, Y. (2015). Intracellular and Organic miRNA In Situ Detection. In: MicroRNA Detection and Pathological Functions. SpringerBriefs in Molecular Science. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-47293-4_7

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