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miRNA Electrochemical Detection

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MicroRNA Detection and Pathological Functions

Part of the book series: SpringerBriefs in Molecular Science ((BRIEFSMOLECULAR))

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Abstract

Electrochemical genosensors hold great promise for point-of-care diagnostics and multiplexed platforms for fast, simple, and inexpensive miRNA analysis. A typical electrochemical biosensor consists of an electroactive hybridization indicator and a solid electrode with an immobilized short single-stranded nucleotide probe [1]. Hybridization between the probe and the complementary sequence influences the electrochemical response signal, producing a signal for detection. The electrochemical miRNA detection methods are based on hybridization, which require translating the hybridization event into a measurable signal when hybridization occurs in the sequence. Sensitivity is challenging for the development of high performance electrochemical genosensors, due to the unique characterization of miRNA. In order to implement the detection of the specific target gene down to attomolar to femtomolar level presented in the organism genome, [2] various approaches are continuously being explored for signal amplification to improve the sensitivity.

Xueji Zhang, Fang Xu and Haifeng Dong contributed together to this chapter.

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Authors and Affiliations

  1. Research Center for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing, China

    Xueji Zhang & Haifeng Dong

  2. Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, China

    Yaping Tian

Authors
  1. Xueji Zhang
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  2. Haifeng Dong
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  3. Yaping Tian
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Correspondence to Xueji Zhang .

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Zhang, X., Dong, H., Tian, Y. (2015). miRNA Electrochemical Detection. In: MicroRNA Detection and Pathological Functions. SpringerBriefs in Molecular Science. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-47293-4_4

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