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Methods in Biological Fuel Cells

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Springer Handbook of Electrochemical Energy

Part of the book series: Springer Handbooks ((SHB))

Abstract

This chapter details the common analytical methods used to evaluate all types of biological fuel cells. These include in situ and ex situ techniques for studying the catalyst, the bioelectrodes, and the complete biological fuel cell. Spectroscopic methods include spectrophotometric kinetic assays, product analysis assays, and electrode characterization techniques. Electrochemical methods include methods for proving bioelectrocatalysis via voltammetry, studying biocatalyst kinetics via amperometry, and performing polarization and power curve measurements on complete biological fuel cells.

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Abbreviations

ATR:

attenuated total reflection

BCA:

bicinchoninic acid

BSA:

bovine serum albumin

DC:

direct current

DCIP:

dichlorophenolindophenol

EIS:

electrochemical impedance spectroscopy

emf:

electromotive force

FAD:

flavin adenine dinucleotide

FTIR:

Fourier-transform infrared

GC-MS:

gas chromatography-mass spectrometry

LC-MS:

liquid chromatography-mass spectrometry

MS:

mass spectrometry

NAD:

nicotinamide adenine dinucleotide

NMR:

nuclear magnetic resonance

PEM:

polymer electrolyte membrane

PMS:

phenazine methosulfate

PQQ:

pyrroloquinoline quinone

QCM:

quartz crystal microbalance

UV-Vis:

ultraviolet–visible

XPS:

x-ray photoelectron spectroscopy

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Authors and Affiliations

  1. Dept. Chemistry and Dept. Materials Science and Engineering, University of Utah, 315 S 1400 E, UT 84112, Salt Lake City, Utah, USA

    Shelley D. Minteer

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  1. Dept. Mechanical Engineering, TU Dresden, Helmholtzstr. 14, 01062, Dresden, Germany

    Cornelia Breitkopf

  2. Chemistry Division, US Naval Research Laboratory, 4555 Overlook Ave., DC 20375, Washington, USA

    Karen Swider-Lyons

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Minteer, S.D. (2017). Methods in Biological Fuel Cells. In: Breitkopf, C., Swider-Lyons, K. (eds) Springer Handbook of Electrochemical Energy. Springer Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-46657-5_22

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