Abstract
An antagonistic bacterial strain named J12 against the grape downy mildew was isolated from 92 bacteria on grape leaves. Antagonistic strains were isolated by exploiting streak plate method. J12 was identified based on morphology observation, physiological and antibacterial characterizations, 16S rDNA sequence analysis, and the phylogenetic tree. The controlling effect of J12 on grape downy mildew was 78.6 %. The liquid fermentation medium of J12 was optimized with the response surface methodology to improve the number of live bacteria. The results showed that the strain J12 was classified as Bacillus amyloliquefaciens. For its liquid fermentation medium, the optimum carbon source was sucrose, the best nitrogen source was soybean meal, and the best C/N ratio was 3:1. By Plackett–Burman design and response surface methodology, the best culture medium formula is 31.18 g/L sucrose, 10.57 g/L soybean meal, 2.206 g/L K2HPO4, 2.5 g/L yeast, 0.5 g/L MgSO4·7H2O. Theoretic optimum cell number was 90.87 × 108 cfu/mL. The number of bacteria in three parallel experiments was 92.54 × 108 cfu/mL, indicating that the equation fits well to the actual production.
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References
Dihazi Abdelhi, Jaiti Fatima, WafaTaktak et al (2012) Use of two bacteria for biological control of bayoud disease caused by Fusarium oxysporum in date palm (Phoenix dactylifera L) seedlings. Plant Physiol Biochem 55:7–15
Bhagwat PR, Sasmita D, Pradeep KD et al (2014) Optimization of extracellular chromate reductase production by Bacillus amyloliquefaciens (CSB 9) isolated from chromite mine environment. Biocatalysis and Agricultural Biotechnology 3(3):1878–8181
Devendra KC, Bhavdish NJ (2008) Interactions of Bacillus spp. and plants-With special reference to induced systemic resistance (ISR). Microbiol Res 164(5):493–513
Dhanya G, Swetha S, Madhavan Nampoothiri K et al (2008) Response surface methodology for the optimization of alpha amylase production by Bacillus amyloliquefaciens. Bioresour Technol 99(11):4597–4602
Gobbin D, Jermini M, Loskill B et al (2005) Importance of secondary inoculum of Plasmopara viticola to epidemics of grapevine downy mildew. Plant Pathol 54:522–534
Lin L, Wang XP, Wang YJ (2006) cDNA clone, fusion expression and purification of the novel gene related to ascorbate peroxidase from Chinese wild Vitis pseudoreticulata in E. coli. Mol Biol Rep 33:197–206
Tari C, Genckal H, Tokatli F (2006) Optimization of a growth medium using a statistical approach for the production of an alkaline protease from a newly isolated Bacillus sp.L21. Process Biochem 41:659–665
Hq Wu, Tian L, Zhang JM et al (2005) Culture medium optimization for pigment production with RSM method. Acta Oceanologica Sinica 5:149–153
Acknowledgments
This work was supported by the Hengshui University Subject for Servicing Local Economic (No. 2013041) and National Nonprofit Sector (Agriculture) Scientific Research (201203035). We also thank for the support of postdoctoral research station in Shenyang Agricultural University.
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Guo, J., Ma, G., Zhu, H., Fu, J. (2015). Isolation and Identification of a Bacillus amyloliquefaciens Strain Against Grape Downy Mildew and Optimization for Its Liquid Fermentation Medium. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 333. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-46318-5_43
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DOI: https://doi.org/10.1007/978-3-662-46318-5_43
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