Abstract
In the previous work, we screened a strain (Bacillus licheniformis JXC-1) producing solvent-stable (10 % N,N-Dimethylformamide, short for DMF) α-glucosidase from several different soil samples. In this work, we attempt to improve the α-glucosidase production by optimization of feeding strategies in a 3-L fermenter. Specifically, the key factors of solvent-tolerant α-glycosidase production were investigated first, and the optimal conditions (pH 7.0, the initial maltose concentration 25 g/L, and agitation speed 600 rpm) were obtained; the enzyme activity reached 444.7 U/L under the optimal conditions. Then, four feeding strategies with different feeding rates for 4–8 h to feed maltose or both maltose and tryptone were carried out, and it was found that feeding maltose and tryptone at a rate of 2.25 mL/h (4–5 h), 6.75 mL/h (5–6 h), 9 mL/h (6–7 h), and 15 mL/h (7–8 h) significantly increased the α-glycosidase production, from 444.7 to 872.5 U/L.
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Carvalho AFA, Martin N, Da Silva R et al (2009) Properties of the purified glycosylated alpha-glucosidase produced by thermophilic fungus Thermoascus aurantiacus CBMAI 756 in submerged fermentation. New Biotechnol 25:S48–S48. doi:10.1016/j.nbt.2009.06.254
Cihan AC, Ozcan B, Cokmus C (2009) Characterization of thermostable α-glucosidases from newly isolated Geobacillus sp. A333 and thermophilic bacterium A343. World J Microbiol Biotechnol 25:2205–2217. doi:10.1007/s11274-009-0127-y
Sato F, Okuyama M, Nakai H et al (2005) Glucoamylase originating from Schwanniomyces occidentalis is a typical α-glucosidase. Biosci Biotechnol Biochem 69:1905–1913
Shimba N, Shinagawa M, Hoshino W et al (2009) Monitoring the hydrolysis and transglycosylation activity of alpha-glucosidase from Aspergillus niger by nuclear magnetic resonance spectroscopy and mass spectrometry. Anal Biochem 393:23–28. doi:10.1016/j.ab.2009.06.002
Carvalho AFA, Boscolo M, Da Silva R et al (2010) Purification and characterization of the alpha-glucosidase produced by thermophilic fungus Thermoascus aurantiacus CBMAI 756. J Microbiol 48:452–459. doi:10.1007/s12275-010-9319-2
Da Silva TM et al (2009) Purification and biochemical characterization of a novel alpha-glucosidase from Aspergillus niveus. Antonie Van Leeuwenhoek 96:569–578. doi:10.1007/s10482-009-9372-1
Carvalho AFA, Leite RSR, Martins E et al (2007) Partially purified and characterization of the α-glucosidase produced by thermophilic fungus Thermoascus aurantiacus CBMAI 756 in submerged fermentation. J Biotechnol 131:S232. doi:10.1016/j.jbiotec.2007.07.422
Tang XY, Pan Y, Li S et al (2008) Screening and isolation of an organic solvent-tolerant bacterium for high-yield production of organic solvent-stable protease. Bioresour Technol 99:7388–7392. doi:10.1016/j.biortech.2008.01.030
Gupta A, Khare SK (2009) Enzymes from solvent-tolerant microbes: useful biocatalysts for non-aqueous enzymology. Crit Rev Biotechnol 29:44–54. doi:10.1080/07388550802688797
Klibanov AM (2001) Improving enzymes by using them in organic solvents. Nature 409:241–246
Karadzic I, Masui A, Zivkovic LI et al (2006) Purification and characterization of an alkaline lipase from Pseudomonas aeruginosa isolated from putrid mineral cutting oil as component of metalworking fluid. J Biosci Bioeng 102:82–89. doi:10.1263/jbb.102.82
Gupta A, Khare SK (2007) Enhanced production and characterization of a solvent stable protease from solvent tolerant Pseudomonas aeruginosa PseA. Enzym Microbial Technol 42:11–16. doi:10.1016/j.enzmictec.2007.07.019
Chen Z, Xueming W, Bingfang H (2010) Development of glycosidase biocatalysis in non-aqueous phase. Chem Ind Eng Prog 29:1292–1299
Zhou L, Li J, Liu L et al (2013) Screening a new α-glucosidase dimethylformamide-tolerant and optimization the transformation conditions of biosynthesising quercetin-d-glucopyranosides Industrial. Microbiology 43:8–13
Hung VS et al (2005) Alpha-Glucosidase from a strain of deep-sea Geobacillus: a potential enzyme for the biosynthesis of complex carbohydrates. Appl Microbiol Biotechnol 68:757–765. doi:10.1007/s00253-005-1977-3
Saqib AAN, Whitney PJ (2011) Differential behaviour of the dinitrosalicylic acid (DNS) reagent towards mono- and di-saccharide sugars. Biomass Bioenergy 35:4748–4750. doi:10.1016/j.biombioe.2011.09.013
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Fang, J., Tang, Q., Liu, L., Li, J. (2015). Enhanced Solvent-Stable Alpha Glycosidase Production by Bacillus licheniformis JXC-1 by Optimization of Feeding Strategies. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 333. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-46318-5_34
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DOI: https://doi.org/10.1007/978-3-662-46318-5_34
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