Abstract
To quickly evaluate the immunostimulatory effect of Astragalus membranaceus polysaccharide (AMP) on yellow drum Nibea albiflora, the head kidney lymphocytes proliferation was tested with/without concanavalin A (Con A) or lipopolysaccharide (LPS) in vitro. The lymphocytes were incubated within 0, 25, 50, 100, 200, 400, and 800 µg/mL AMP for 48 h, with/without Con A (2.3 µg/mL) or LPS (18.2 µg/mL). Results showed that there was a dose-dependent relationship between lymphocytes proliferation and the AMP concentration (P < 0.05). The AMP-enhanced lymphocytes proliferation at 50–200 µg/mL significantly (P < 0.05), with peak value at 100 µg/mL. Low dose (0–25 µg/mL) and high dose (400–800 µg/mL) of AMP did not stimulate lymphocytes proliferation significantly (P < 0.05). Synergetic effects were observed between AMP and Con A/LPS on lymphocytes proliferation. Data in this chapter implied that the AMP might contribute to both cellular and humoral immunity of N. albiflora in a dose-dependent manner.
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The financial support provided by Science and Technology Agency of Zhejiang province (Grant No. 2012C12907-8) is gratefully acknowledged.
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Shi, H., Yu, F., Wang, Q. (2015). Astragalus membranaceus Polysaccharide-Enhanced Lymphocytes Proliferation of Yellow Drum Nibea albiflora In Vitro. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 333. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-46318-5_27
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DOI: https://doi.org/10.1007/978-3-662-46318-5_27
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