Abstract
In the paper, in order to improve the polyunsaturated fatty acids (PUFAs) by Mortierella isabellina, the effects of carbon source, nitrogen source, carbon to nitrogen (C/N) ratio, and metal ions on PUFAs production were investigated. The results of the research showed that different kinds of carbon source and nitrogen source had obviously effect on PUFAs production, the glucose and mixture nitrogen (yeast extract: peanut meal at 3:1 (w/w)) were greatly suitable for PUFAs production and cell growth. The glucose concentration and C/N ratios have significant effect on PUFAs production. Meanwhile, the results indicate the metal ions have profound effects on fungus growth and PUFAs production. Ca2+ and Mg2+ could promote PUFAs production. Almost all the improvement of PUFAs was obtained through increasing thallus biomass, though there were some influences on the metabolism. Under the optimal culture media composition, the yields of cell dry weight, linoleic acid (LA), gama-linolenic acid (GLA), and total PUFAs were 32.48, 2.90, 2.83, and 7.36 g/L, respectively.
D. Sun and C. Zhou contributed equally to this work.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Virginie TA, Kari LK, Maoa JQ et al (2012) Supercritical carbon dioxide extraction of polyunsaturated fatty acids from Northern shrimp (Pandalus borealis Kreyer) processing by-products. Food Chem 130:853–858
Eiji S, Akinori A, Sakayu S et al (2013) B Metabolic engineering for the production of polyunsaturated fatty acids by oleaginous fungus Mortierella alpina 1S-4. J Biosci Bioeng http://dx.doi.org/10.1016/j.jbiosc.2013.04.008
Subramaniam R, Dufreche S, Zappi M et al (2010) Microbial lipids from renewable resources: production and characterization. J Ind Microbiol Biot 37(12):1271–1287
Fakas S, Certik M, Papanikolaou S et al (2008) Gamma-Linolenic acid production by Cunninghamella echinulata growing on complex organic nitrogen sources. Bioresour Technol 99:5986–5990
Muammer D, Irfan T, Ahmet K (2013) Oil production by Mortierella isabellina from whey treated with lactase. Bioresour Technol 128(2013):365–369
Zhu CH, Lu FP, He YN et al (2007) Statistical optimization of medium components for avilamycin production by Streptomyces viridochromogenes Tü57-1 using response surface methodology. J Ind Microbiol Biotechnol 34:271–278
Bligh EC, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
Ministry of Health of the People’s Republic of China. Standards for making fatty acid methyl ester, GB/T 17376-2008
Jang HD, Lin YY, Yang SS (2000) Polyunsaturated fatty acid production with Mortierella alpina by solid state fermentation. Bot Bull Acad Sinicia 41:41–48
SAS Institute (2002) SAS user’s guide statistics. SAS Institute Inc., Cary
Papanikolaou S, Sarantou S, Komaitis M (2004) Repression of reserve lipid turnover in Cunninghamella echinulata and Mortierella isbellina cultivated in multiple-limited media. Appl Microbiol 97:867–875
Xian M, Bi YL, Zhen KJ (2002) Production of linolenic acid by Mortierella isabellina Grown on Octadecanol. Current Microbiol 44:141–144
Jing HD, Lin YY, Yang SS (2005) Effect of culture media and conditions on polyunsaturated fatty acids production by Mortierella alpina. Bioresour Technol 96:1633–1644
Enoch Y, Park L, Yasuhisa K et al (1999) Effect of nitrogen source on mycelial morphology and arachidonic acid production in cultures of mortierella alpine. J Biosci Bioeng 88(1):61–67
Liu B, Sun Y, Zhao ZB (2005) Research progress of lipids biosynthesis and metabolic regulation with oleaginous organisms. J Acta Microbiolaogica Sinica 45(1):153–155
Evans CT, Scragg AH, Ratledge C (1983) Regulation of citrate efflux from mitochondria of oleaginous and non-oleaginous yeasts by adenine nucleotides. J Biochem 132:609–615
Weete JD, Kim H, Gandhi SR (1997) Lipids and ultrastructure of Thraustochytrium sp ATCC 26185. Lipids 8:839–845
Wang AQ, Dai CC, Chen JX (2005) Study of the effect of six metal ions on the growth and ARA production. Food Chem 26(9):67–71
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Sun, D., Zhou, C., Zhu, C., Sun, Y. (2015). Effects of Culture Medium on PUFAs Production by Mortierella isabellinas . In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 333. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-46318-5_24
Download citation
DOI: https://doi.org/10.1007/978-3-662-46318-5_24
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-46317-8
Online ISBN: 978-3-662-46318-5
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)