Abstract
Adenosine has potent effects on cardiovascular diseases and has been widely used as an antiarrhythmic agent. To improve its production, we investigated the effects of dissolved oxygen (DO) and pH on production by Bacillus subtilis. Based on the kinetic parameters at different DO levels, we proposed a two-stage DO strategy to control DO level at 30–40 % before 20 h of fermentation and 10–20 % after 20 h of fermentation, and confirmed that using this strategy could increase adenosine yield to 19.2 g/L in 52 h, which is increased by 78.6, 66.7, 9.5, 18.6, and 32.2 %, compared to the conditions with DO uncontrolled or controlled at 0–10, 10–20, 20–30, and 30–40 %, respectively. On this basis, pH was adjusted to further boost adenosine production. The results showed that the two-stage DO plus pH-shift method was the optimal way for adenosine production, under which, adenosine yield was further improved by 21.4 %, reaching 23.3 g/L at 56 h. To our knowledge, this is the first report on adenosine production verifying that the two-stage DO plus pH-shift method is effective for enhancing adenosine yield by B. subtilis.
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Acknowledgments
We gratefully acknowledge the excellent support of Shanshan Du in their operation of the fermentation process control. This investigation is supported by the National Natural Science Foundation of China (31100054) and by Program for Changjiang Scholars and Innovative Research Team in University (IRT 1166).
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Liu, Y., He, J., Xu, Q., Zhang, C., Chen, N., Xie, X. (2015). Enhanced Adenosine Production by Bacillus subtilis at Condition with Comprehensively Controlled Dissolved Oxygen and pH During Fermentation. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 332. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45657-6_46
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