Abstract
Pichia pastoris GS115 displaying Aspergillus aculeatus β-glucosidase I was used as whole-cell biocatalyst catalyzing the synthesis of 1-hexyl glucoside, 1-octyl glucoside, and 1-decyl glucoside through reverse hydrolysis in water–alcohol two-phase system, while glucose and primary alcohols were used as substrate. In this study, factors affecting the synthetic yield were optimized in our study, including water content, glucose concentration, whole-cell biocatalyst content, the pH value of sodium acetate buffer, and the reaction temperature. In 5 mL reaction volume, the optimal reaction conditions of HG were: 10 % pH 3.0 buffer, 0.2 mol/L glucose, 10 g/L whole-cell biocatalyst; the optimal reaction conditions of DG: 15 % pH 3.0 buffer, 0.6 mol/L glucose, 10 g/L whole-cell biocatalyst; the optimal reaction conditions of DG: 20 % pH 2.0 buffer, 0.2 mol/L glucose, 40 g/L whole-cell biocatalyst; three reactions were conducted under 55 °C. After 72 h the maximum yield of HG was 11.5 %, that of OG and DG were 5.3 and 3.2 % after 96 h, respectively. The reutilization experiments inducted that the whole-cell biocatalyst could be used at least for five times without obvious decrease in synthesis ability.
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Kang, Y., Wei, B., Guo, D., Zheng, S. (2015). Production of Alkyl Polyglucoside Using Pichia pastoris GS115 Displaying Aspergillus aculeatus β-Glucosidase I. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 332. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45657-6_44
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DOI: https://doi.org/10.1007/978-3-662-45657-6_44
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