Abstract
Microbial transformation of 13β-alkyl-3-methoxy-8, 14, secogona-1, 3, 5(10), 9-tetraen-14, 17-dion with Saccharomyces cerevisiae resulted in two metabolites: product 1 (P1) and product 2 (P2). Among these, P1 was identified to be 13β alkyl-3-methoxy-8, 14-seco-1, 3, 5(10), 9(11)-estratetraene-17β-ol, 14-one, which has been studied extensively. P2 was purified, crystallized, and identified as 13β-alkyl-3-methoxy-8, 14, secogona-1, 3, 5(10), 9-tetraen-14, 17-diol by X-ray single crystal diffraction method. It was first reported in fermentation medium and the dihydroxyl reduction is a new reaction in biotransformation of steroid.
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Acknowledgments
This work was supported financially by Natural Science Foundation of China (No. 21206127) and Applied Basic Research Programs of Science and Technology Commission Foundation of Tianjin (No.12JCQNJC06400).
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Yu, L., Mao, S., Ji, S., Liu, X., Lu, F. (2015). Reduction Reaction of Methyl Condensation Compound by Saccharomyces cerevisiae . In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 332. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45657-6_37
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DOI: https://doi.org/10.1007/978-3-662-45657-6_37
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