Abstract
A peanut mutant with 72.53 % oleate content was selected from sodium azide mutagenized peanut Huayu 22, an export type cultivar with 51.65 % oleate content. The chemical mutant had a point mutation (C281T) in the coding region of FAD2B, which caused a T94I substitution in the FAD2B protein. Though the mutated FAD2B gene was incompletely dysfunctional as shown in yeast expression system, the peanut mutant has a high oleic acid phenotype. This indicated the possible involvement of other unidentified genetic factor(s) conditioning oleic acid content in peanut.
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Acknowledgments
We would like to thank China Agricultural Research System (Grant No. CARS-14) and SAAS (Shandong Academy of Agricultural Sciences) Key Science and Technology Achievements Incubation Project (2014CGPY09) for providing financial support for this study.
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Wang, X.Z., Wu, Q., Tang, Y.Y., Sun, Q.X., Wang, C.T. (2015). FAD2B from a Peanut Mutant with High Oleic Acid Content Was Not Completely Dysfunctional. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 332. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45657-6_28
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DOI: https://doi.org/10.1007/978-3-662-45657-6_28
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