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Advances in Applied Biotechnology pp 233–243Cite as

Effects of Calcium on the Morphology of Rhizopus oryzae and L-lactic Acid Production

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Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 332))

Abstract

The effects of exogenous calcium on fungal pellet morphology during preculture and L-lactic acid production were studied. The results showed that addition of exogenous calcium could induce pellet formation. The diameter of the pellet increased with increasing concentration of exogenous calcium, including CaCl2 and CaCO3. The smaller pellet precultured with low concentration of soluble calcium (CaCl2) was beneficial for L-lactic acid production because the pellet was dense and the large inner part of the pellet was inactive. By contrast, the larger pellet precultured with high concentration of insoluble calcium (CaCO3), except 8.0 g/L CaCO3, was beneficial for L-lactic acid production. Supported by the CaCO3 powder, the entire biomass layer was fully active, and the highest L-lactic acid productivities of 1.22 g/L h and 58.6 g/L L-lactic acid were reached using the 1.5 mm pellet.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (Grant No. 21106091), Zhejiang Provincial Natural Science Foundation of China (LQ12B06004).

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Authors and Affiliations

  1. Institute of Biomass Resources, Taizhou University, Jiaojiang, 318000, Zhejiang, People’s Republic of China

    Yong-Qian Fu, Long-Fei Yin, Ru Jiang, Hua-Yue Zhu & Qing-Cheng Ruan

Authors
  1. Yong-Qian Fu
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  2. Long-Fei Yin
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  3. Ru Jiang
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  4. Hua-Yue Zhu
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  5. Qing-Cheng Ruan
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Corresponding author

Correspondence to Yong-Qian Fu .

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Editors and Affiliations

  1. Tianjin University of Science and Technology, Tianjin, China

    Tong-Cun Zhang

  2. SBI ALApromo, Tokyo, Japan

    Motowo Nakajima

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Fu, YQ., Yin, LF., Jiang, R., Zhu, HY., Ruan, QC. (2015). Effects of Calcium on the Morphology of Rhizopus oryzae and L-lactic Acid Production. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 332. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45657-6_25

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