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Mega-Genome DNA Extraction from Pit Mud

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Advances in Applied Biotechnology

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 332))

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Abstract

Molecular analyses for the study of microbial community often depend on the direct extraction of the mega-genome DNA from pit mud. The present work compared six different methods to extract the mega-genome DNA from pit mud, and investigated the relationship between the quantity and the diversity of the mega-genome DNA. Using the six different extraction methods, the DNA ranged from 3.18 to 20.17 ng DNA/μL. Both the quantity and the diversity of the mega-genome DNA, the “repeated freezing and thawing” method is superior to other five methods, so it is the best choice for the extraction of mega-genome DNA from pit mud.

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Acknowledgments

This work was funded by the doctoral program fund from Chinese Ministry of Education (20121501120001).

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Authors and Affiliations

  1. College of Life Science, Inner Mongolia University, Huhhot, China

    Huimin Xie, Yali Dai & Lin Yuan

Authors
  1. Huimin Xie
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  2. Yali Dai
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  3. Lin Yuan
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Corresponding author

Correspondence to Lin Yuan .

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Editors and Affiliations

  1. Tianjin University of Science and Technology, Tianjin, China

    Tong-Cun Zhang

  2. SBI ALApromo, Tokyo, Japan

    Motowo Nakajima

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Xie, H., Dai, Y., Yuan, L. (2015). Mega-Genome DNA Extraction from Pit Mud. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 332. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45657-6_16

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