Abstract
Enduracidin is a kind of lipodepsipeptides and composed by a 17 amino acid residual conjugated with a fatty acid strain. It is used as a feed additive approved by EU because of its high safety, low toxicity, low-residue, and activity against a wide variety of Gram-positive bacteria. Here, we combined a physical mutagenesis of 137Cs γ-irradiation with the atmospheric and room temperature plasma (ARTP) biological breeding system to improve enduracidin productivity in the industrial Streptomyces. The mutation rates of spores treated by ARTP for 1 min and by 137Cs γ-irradiation for 500 Gy dosages were 26.51 and 19.41 %, respectively. An optimal process for breeding by the combination was proposed. Through the high throughout screening method, the mutant Streptomyces fungicidicus S-224 was obtained, and its enduracidin yield reached to 1.58 g/L that was 1.65-fold to the original strain. The mutation breeding combined ARTP with 137Cs γ rays was the first trial and provided an alternative method for industrial strains improvement.
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Acknowledgments
We thank the technical support by Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China, and Zhejiang Academy of Agricultural Sciences. The work was financially supported by College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, China.
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Zhang, D., Wang, Q., Liang, X. (2015). Breeding High Producers of Enduracidin from Streptomyces fungicidicus by Combination of Various Mutation Treatments. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 332. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45657-6_14
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DOI: https://doi.org/10.1007/978-3-662-45657-6_14
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