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Improved Lactose Utilization by Overexpression β-Galactosidase and Lactose Permease in Klebsiella pneumoniae

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Advances in Applied Biotechnology

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 332))

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Abstract

As an important bio-based chemical product, 2,3-butanediol has a wide range of applications in many fields, such as chemical, fuel, food, and aerospace. Cheese whey powder (CWP), an inexpensive, available, and abundant material, is considered to be an ideal substrate for 2,3-BD fermentation. To improve 2,3-butanediol production, the previous studies mainly focus on the metabolic pathway from pyruvate to 2,3-butanediol or the metabolic pathway of by-products, but studies about improving lactose utilization rate are rarely reported. In the present study, adding exogenous β-galactosidase was proved to favor the lactose utilization and lactose utilization might be the limiting step of lactose fermentation to 2,3-butanediol. ElacY (encoding lactose permease of Escherichia coli) and bgaB (encoding β-galactosidase of K. pneumonia) were overexpressed in K. pneumonia CICC10781. Of the two genes, only overexpression of ElacY promoted lactose utilization of CICC10781, and meanwhile the 2,3-butanediol generation capacity was not affected.

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Acknowledgments

This research was financed by the Cheung Kong Scholars and Innovative Research Team Program in University of Ministry of Education, China (Grant Number IRT1166), the National High Technology Research, and the Development Program of China (863 Program) (Grant Number 2012AA022108), the National Agricultural Research Projects Funded (Grant Number 2012AA101805).

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Authors and Affiliations

  1. Key Laboratory of Industrial Fermentation Microbiology Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Xuewu Guo, Yazhou Wang, Xiangyu Guan, Yefu Chen, Cuiying Zhang & Dongguang Xiao

Authors
  1. Xuewu Guo
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  2. Yazhou Wang
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  3. Xiangyu Guan
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  4. Yefu Chen
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  5. Cuiying Zhang
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  6. Dongguang Xiao
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Corresponding author

Correspondence to Dongguang Xiao .

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Editors and Affiliations

  1. Tianjin University of Science and Technology, Tianjin, China

    Tong-Cun Zhang

  2. SBI ALApromo, Tokyo, Japan

    Motowo Nakajima

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Guo, X., Wang, Y., Guan, X., Chen, Y., Zhang, C., Xiao, D. (2015). Improved Lactose Utilization by Overexpression β-Galactosidase and Lactose Permease in Klebsiella pneumoniae . In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 332. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45657-6_13

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