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Advances in Applied Biotechnology pp 109–119Cite as

Isolation and Identification of a Cellulose-Producing Bacterial Strain from the Genus Bacillus

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Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 332))

Abstract

Strain ZF-7 was isolated from defective fruits in this study, and its product was determined as bacterial cellulose in view of the results of cellulose special reaction, FTIR spectroscopy analysis, and cellulose hydrolysis experiments. Based on morphological, physiological, and biochemical characteristics, ZF-7 was identified by 16S rDNA sequences and systematic analysis. The results indicated that 16S rDNA sequence of ZF-7 had 99.5 % similarity to the partial sequence of Bacillus amyloliquefaciens, suggesting that ZF-7 was categorized as a species of B. amyloliquefaciens, and denominated as B. amyloliquefaciens ZF-7. The fermentation performance of ZF-7 under shaking and static flasks was inspected preliminary, and the BC production was 6.6 and 6.2 g/L, respectively.

Hongyang Zhu and Yang Zhang had equal contributions to this work.

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References

  1. Yu B, Zhou HL (2007) Progress in research of bacterial cellulose. Biotechnol Bull 2:87–90

    Google Scholar 

  2. Brown RM (2004) Cellulose structure and biosynthesis: what is in store for the 21st century? J Polym Sci Part A: Polym Chem 42:487–495

    Article  CAS  Google Scholar 

  3. Gullo M, Giudici P (2008) Acetic acid bacteria in traditional balsamic vinegar: phenotypic traits relevant for starter cultures selection. Int J Food Microbiol 125:46–53

    Article  CAS  Google Scholar 

  4. Jonas R, Farah LF (1998) Production and application of microbial cellulose. Polym Degrad Stab 59(1):101–106

    Article  CAS  Google Scholar 

  5. Jeong SI, Lee SE, Yang H et al (2010) Toxicologic evaluation of bacterial synthesized cellulose in endothelial cells and animals. Mol and Cell Toxicol 6(4):370–377

    Article  CAS  Google Scholar 

  6. Azuma C, Yasuda K, Tanabe Y et al (2006) Biodegradation of high-toughness double network hydrogels as potential material for artificial cartilage. J Biomed Res Part A 81(2):373–380

    Google Scholar 

  7. Mori R, Nakai T, Enomoto K et al (2011) Increased antibiotic release from a bone cement containing bacterial cellulose. Clin Orthop Relat Res 469(2):600–606

    Article  Google Scholar 

  8. Wang J, Gao C, Zhang Y et al (2010) Preparation and in vitro characterization of BC/PVA hydrogel composite for its potential use as artificial cornea biomaterial. Mater Sci Eng: C 30(1):214–218

    Article  Google Scholar 

  9. Shi ZJ, Zhang Y, Philips GO et al (2014) Utilization of bacterial cellulose in food. Food Hydrocolloids 35:539–545

    Article  CAS  Google Scholar 

  10. Rani MU, Udayasankar K, Appaiah KA (2011) Properties of bacterial cellulose produced in grape medium by native isolate Gluconacetobacter sp. J Appl Polym Sci 120(5):2835–2841

    Article  CAS  Google Scholar 

  11. Wu RQ, Li ZX, Yang JP et al (2010) Mutagenesis induced by high drostatic pressure treatment: a useful method to improve the bacterial cellulose yield of a Gluconoacetobacter xylinus strain. Cellulose 17(2):399–405

    Article  CAS  Google Scholar 

  12. Son HJ, Kim HG, Kim KK et al (2003) Increased production of bacterial cellulose by Acetobacter sp. V6 in synthetic media under shaking culture conditions. Bioresour Technol 86(3):215–219

    Article  Google Scholar 

  13. Cheng KC, Catchmark JM, Demirci A (2009) Effect of different additives on bacterial cellulose production by Acetobacter xylinum and analysis of material property. Cellulose 16:1033–1045

    Article  CAS  Google Scholar 

  14. Bodin A, Backdahl H, Fink H (2007) Influence of cultivation conditions on mechanical and morphological properties of bacterial cellulose tubes. Biotechnol Bioeng 97:425–434

    Article  CAS  Google Scholar 

  15. Jipa IM, Stoica-Guzun A, Stroescu M (2012) Controlled released of sorbic acid from bacterial cellulose based mono and multilayer antimicrobial films. LWT-Food Sci and Technol 47(2):400–406

    Article  CAS  Google Scholar 

  16. Sureshkumar M, Siswanto DY, Lee C (2010) Magnetic antimicrobial nanocomposite based on bacterial cellulose and silver nanoparticles. J Mater Chem 20(33):6948–6955

    Article  CAS  Google Scholar 

  17. George MG, Julia AB, Timothy GL (1984) Bergey’s manual of systematic bacteriology (In: Chinese translation). Science Press, Beijing

    Google Scholar 

  18. Shi QS, Feng J, Feng J et al (2009) Isolation and identification of a cellulose-producing bacterial strain. Fajiao Keji Tongxun 38:11–15

    Google Scholar 

  19. Liu JL (2002) Instrumental Analysis. Science Press, Beijing

    Google Scholar 

Download references

Acknowledgments

This work was supported by the Natural Science Foundation of Fujian Province of China (No. 2012J05015), Educational Commission of Fujian Province of China (No. JA12430, JA13414), and Doctoral Research Startup Foundation of Liaocheng University of China (No. 31805).

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Authors and Affiliations

  1. Department of Pharmacy, Fujian Health College, Fuzhou, 350101, Fujian, China

    Hongyang Zhu, Jinhai Wang, Yongning Li & Weiling Lin

  2. College of Life Science, Liaocheng University, Liaocheng, 252059, China

    Yang Zhang

Authors
  1. Hongyang Zhu
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  2. Yang Zhang
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  3. Jinhai Wang
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  4. Yongning Li
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  5. Weiling Lin
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Corresponding author

Correspondence to Hongyang Zhu .

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Editors and Affiliations

  1. Tianjin University of Science and Technology, Tianjin, China

    Tong-Cun Zhang

  2. SBI ALApromo, Tokyo, Japan

    Motowo Nakajima

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© 2015 Springer-Verlag Berlin Heidelberg

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Zhu, H., Zhang, Y., Wang, J., Li, Y., Lin, W. (2015). Isolation and Identification of a Cellulose-Producing Bacterial Strain from the Genus Bacillus . In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 332. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45657-6_12

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