Abstract
Sphingomonas sanxanigenens strain NX02 synthesizes a novel sphingan Ss, which can be used as drilling mud and thickening agent in the recovery of petroleum by water flooding. In order to research genes involved in the biosyntheses of sphingan Ss, strain NX02 was induced by transposon mini-Tn5 on suicide plasmid pUT, and a mutant strain T163, which cannot produce sphingan Ss, was screened. The spsC gene of NX02 was obtained by the method of Tn5 flanking PCR and LP-RAPD. The predicted amino acid sequence of the spsC protein possessed 493 amino acids and a calculated molecular mass of 53.5 kDa. Bioinformatics analysis revealed that spsC had the highest 60 % amino acid sequence identity with polysaccharide biosynthesis protein of Novosphingobium lindaniclasticum LE124. spsC protein had typical polysaccharide polymerases family transmembrane helices, located between amino acids Y13-V44 and P411-L436. The N-terminal sequence of spsC had high identity to chain length determinant protein of Wzz superfamily.
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References
Pollock TJ (1993) Gellan-related polysaccharides and the genus Sphingomonas. J Gen Microbiol 139:1939–1945
Sá-Correia I, Fialho AM, Videira P, Moreira LM, Marques AR, Albano H (2002) Gellan gum biosynthesis in Sphingomonas paucimobilis ATCC 31461: genes, enzymes and exopolysaccharide production engineering. J Ind Microbiol Biotechnol 29:170–176
Yabuuchi E, Yano I, Oyaizu H, Hashimoto Y, Ezaki T, Yamamoto H (1990) Proposals of Sphingomonas paucimobilis gen. nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov., Sphingomonas adhaesiva sp. nov., Sphingomonas capsulata comb. nov., and two genospecies of the genus Sphingomonas. Microbiol Immunol 34:99–119
Prajapati VD, Jani GK, Zala BS, Khutliwala TA (2013) An insight into the emerging exopolysaccharide gellan gum as a novel polymer. Carbohydr Polym 93:670–678
Smith AM, Shelton RM, Perrie Y, Harris JJ (2007) An initial evaluation of gellan gum as a material for tissue engineering applications. J Biomater Appl 22:241–254
Banik RM, Kanari B, Upadhyay S (2000) Exopolysaccharide of the gellan family: prospects and potential. World J Microbiol Biotechnol 16:407–414
Ishwar BB, Shrikant AS, Parag SS, Rekha SS (2007) Gellan gum: fermentative production, downstream processing and applications. Food Technol Biotechnol 45:341–354
Seo EJ, Yoo SH, Oh KW, Cha J, Lee HG, Park CS (2004) Isolation of an exopolysaccharide-producing bacterium, Sphingomonas sp. CS101, which forms an unusual type of sphingan. Biosci Biotechnol Biochem 68:1146–1148
Huang HD, Wang W, Ma T, Li GQ, Liang FL, Liu RL (2009) Sphingomonas sanxanigenens sp. nov., isolated from soil. Int J Syst Evol Microbiol 59:719–723
Huang HD, Wang W, Ma T, Li ZY, Liang FL, Liu RL (2009) Analysis of molecular compositioni and properties of a novel biopolymer. Chem J Chin Univ 30:324–327
Yamazaki M, Thorne L, Mikolajczak M, Armentrout RW, Pollock TJ (1996) Linkage of genes essential for synthesis of a polysaccharide capsule in Sphingomonas strain S88. J Bacteriol 178:2676–2687
Coleman RJ, Patel YN, Harding NE (2008) Identification and organization of genes for diutan polysaccharide synthesis from Sphingomonas sp. ATCC 53159. J Ind Microbiol Biotechnol 35:263–274
Li H, Xu H, Xu H, Li S, Ouyang PK (2010) Biosynthetic pathway of sugar nucleotides essential for welan gum production in Alcaligenes sp. CGMCC2428. Appl Microbiol Biotechnol 86:295–303
Fialho AM, Moreira LM, Granja AT, Popescu AO, Hoffmann K, Sá-Correia I (2008) Occurrence, production, and applications of gellan: current state and perspectives. Appl Microbiol Biotechnol 79:889–900
Moreira LM, Hoffmann K, Albano H, Becker A, Niehaus K, Sá-Correia I (2004) The gellan gum biosynthetic genes gelC and gelE encode two separate polypeptides homologous to the activator and the kinase domains of tyrosine autokinases. J Mol Microbiol Biotechnol 8:43–57
Rather PN, Ding X, Lancey RB, Siddiqui S (1999) Providencia stuartii genes activated by cell-to-cell signaling and identification of a gene required for production or activity of an extracellular factor. J Bacteriol 181:7185–7191
Sambrook J, Fritsch EF, Maniatis T (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor, New York
Cashion P, Holder-Franklin MA, McCully J, Franklin M (1977) A rapid method for the base ratio determination of bacterial DNA. Anal Biochen 81:461–466
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410
Higgins DG, Sharp PM (1988) CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene 73:237–244
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Guffin LJ, Bryson K, David TJ (2000) The PSIPRED protein structure prediction server. Bioinform 16:404–405
Videira PA, Fialho AM, Geremia RA, Breton C, Sá-Correia I (2001) Biochemical characterization of the beta-1,4-glucuronosyltransferase GelK in the gellan gum-producing strain Sphingomonas paucimobilis ATCC 31461. Biochem J 358:457–464
Huang HD, Li XY, Wu MM, Wang SX, Li GQ, Ma T (2013) Cloning, expression and characterization of a phosphoglucomutase/phosphomannomutase from sphingan-producing Sphingomonas sanxanigenens. Biotechnol Lett 35:1265–1270
Whitfield C (2006) Biosynthesis and assembly of capsular polysaccharides in Escherichia coli. Ann Rev Biochem 75:39–68
Acknowledgments
The authors are grateful to Tianjin Research Program of Application Foundation and Advanced Technology (11JCZDJC16600) for the financial support for this work.
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Li, X., Huang, H., Zhou, M., Zhang, P. (2015). Cloning and Bioinformatics Analysis of spsC Gene from Sphingomonas sanxanigenens NX02. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 332. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45657-6_1
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