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Microbial Succinic Acid Production Using Different Bacteria Species

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Microorganisms in Biorefineries

Part of the book series: Microbiology Monographs ((MICROMONO,volume 26))

Abstract

Succinic acid (1,4-butanedioic acid) is identified as one of the important building-block chemicals, which can be used for the synthesis of high value-added derivatives, such as 1,4-butanediol (BDO), tetrahydrofuran (THF), g-butyrolactone (GBL), succinimide, and especially the biodegradable material polybutylene succinate (PBS). Confronted with the gradual and inescapable exhaustion of the earth’s fossil energy resources, fermentative succinate production from renewable biomass to replace the conventional petrochemical process is receiving an increasing amount of attention. Great efforts have been made to develop biotechnology methods to produce succinic acid using different bacteria which mainly include the following species, Actinobacillus succinogenes, Escherichia coli, Saccharomyces cerevisiae, Anaerobiospirillum succiniciproducens, Corynebacterium glutamicum, Mannheimia succiniciproducens, and Basfia succiniciproducens. The fermentative succinic acid production has been made commercially available by the joint efforts of researchers in different fields. In this chapter, bacteria species for succinate production, including natural succinate overproducers and recombinant overproducers, are discussed. Besides, latest efforts and experiences devoted to microbial succinic acid production are also summarized, including biomass-based biorefining strategy, in situ product removal process, and novel downstream processing. Finally, the key limitations and challenges faced in recent microbial production systems are also proposed.

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References

  • Agarwal L, Isar J, Dutt K, Saxena RK (2007a) Statistical optimization for succinic acid production from E. coli in a cost-effective medium. Appl Biochem Biotechnol 142:158–167

    Article  CAS  PubMed  Google Scholar 

  • Agarwal L, Isar J, Meghwanshi GK, Saxena RK (2007b) Influence of environmental and nutritional factors on succinic acid production and enzymes of reverse tricarboxylic acid cycle from Enterococcus flavescens. Enzyme Microb Technol 40:629–636

    Article  CAS  Google Scholar 

  • Agren R, Otero JM, Nielsen J (2013) Genome-scale modeling enables metabolic engineering of Saccharomyces cerevisiae for succinic acid production. J Ind Microbiol Biotechnol 40:735–747

    Article  CAS  PubMed  Google Scholar 

  • Andersson C, Hodge D, Berglund KA, Rova U (2007) Effect of different carbon sources on the production of succinic acid using metabolically engineered Escherichia coli. Biotechnol Prog 23:381–388

    Article  CAS  PubMed  Google Scholar 

  • Becker J, Reinefeld J, Stellmacher R, Schäfer R, Lange A, Meyer H, Lalk M, Zelder O, von Abendroth G, Schröder H, Haefner S, Wittmann C (2013) Systems-wide analysis and engineering of metabolic pathway fluxes in bio-succinate producing Basfia Succiniciproducens. Biotechnol Bioeng 110:3013–3023

    Article  CAS  PubMed  Google Scholar 

  • Berglund KA, Yedur S, Dunuwila D (1999) Succinic acid production and purification. Patent US5958744

    Google Scholar 

  • Bomgardner MM (2011) Myriant to build succinic acid plant in Louisiana. Chem Eng News 89:7

    Google Scholar 

  • Bunch PK, Mat-Jan F, Lee N, Clark DP (1997) The ldhA gene encoding the fermentative lactate dehydrogenase of Escherichia coli. Microbiology 143:187–195

    Article  CAS  PubMed  Google Scholar 

  • Chatterjee R, Millard CS, Champion K, Clark DP, Donnelly MI (2001) Mutation of the ptsG gene results in increases production of succinate in fermentation of glucose by Escherichia coli. Appl Environ Microbiol 67:148–154

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Chen KQ, Zhang H, Miao YL, Jiang M, Chen JY (2010) Succinic acid production from enzymatic hydrolysate of sake lees using Actinobacillus succinogenes 130Z. Enzyme Microb Technol 47:236–240

    Article  CAS  Google Scholar 

  • Cheng KK, Zhao XB, Zeng J, Wu RC, Xu YZ, Liu DH, Zhang JA (2012) Downstream processing of biotechnological produced succinic acid. Appl Microbiol Biotechnol 95:841–850

    Article  CAS  PubMed  Google Scholar 

  • Cok B, Tsiropoulos I, Roes AL, Patel MK (2014) Succinic acid production derived from carbohydrates: an energy and greenhouse gas assessment of a platform chemical toward a bio-based economy. Biofuel Bioprod Bior 8:16–29

    Article  CAS  Google Scholar 

  • Datta R, Glassner DA, Jain MK, Vick Roy JR (1992) Fermentation and purification process for succinic acid. Patent US5168055

    Google Scholar 

  • David H, Åkesson M, Nielsen J (2003) Reconstruction of the central carbon metabolism of Aspergillus niger. J Dairy Sci 270:4243–4253

    CAS  Google Scholar 

  • Davis CP, Cleven D, Brown J (1976) Anaerobiospirillum, a new genus of spiral-shaped bacteria. Int J Syst Bacteriol 26:498–504

    Article  Google Scholar 

  • Davison BH, Nghiem NP, Richardson GL (2004) Succinic acid asorption from fermentation broth and regeneration. Appl Biochem Biotechnol 12:653–669

    Article  Google Scholar 

  • Donnelly MI, Millard CS, Clark DP, Chen MJ, Rathke JW (1998) A novel fermentation pathway in an Escherichia coli mutant producing succinic acid, acetic acid, and ethanol. Appl Biochem Biotechnol 70–72:187–198

    Article  PubMed  Google Scholar 

  • Donovan RS, Robinson CW, Glick BR (1996) Review: Optimizing inducer and culture conditions for expression of foreign proteins under the control of the lac promoter. J Ind Microbiol 16:145–154

    Article  CAS  PubMed  Google Scholar 

  • Du CY, Carol Lin SK, Koutinas A, Wang RH, Dorado P, Webb C (2008) A wheat biorefining strategy based on solid-state fermentation for fermentative production of succinic acid. Bioresour Technol 99:8310–8315

    Article  CAS  PubMed  Google Scholar 

  • Efe Ç, van der Wielen LAM, Straathof AJJ (2013) Techno-economic analysis of succinic acid production using adsorption from fermentation medium. Biomass Bioenergy 56:479–492

    Article  CAS  Google Scholar 

  • Elcio RB, Nei PJ (2011) Succinic acid production from sugarcane bagasse hemicellulose hydrolysate by Actinobacillus succinogenes. J Ind Microbiol Biotechnol 38:1001–1011

    Article  Google Scholar 

  • Gallmetzer M, Meraner J, Burgstaller W (2002) Succinate synthesis and excretion by Penicillium simplicissimum under aerobic and anaerobic conditions. FEMS Microbiol Lett 210:221–225

    Article  CAS  PubMed  Google Scholar 

  • Glassner DA, Datta R (1992) Process for the production and purification of succinic acid. Patent US5143834

    Google Scholar 

  • Gokarn RR, Evans JD, Walker JR, Martin SA, Eiteman MA, Altman E (2001) The physiological effects and metabolic alterations caused by the expression of Rhizobium etli pyruvate carboxylase in Escherichia coli. Appl Microbiol Biotechnol 20:188–195

    Article  Google Scholar 

  • Gombert AK, Kilikian BV (1998) Recombinant gene expression in Escherichia coli cultivation using lactose as inducer. J Biotechnol 60:47–54

    Article  CAS  PubMed  Google Scholar 

  • Guettler MV, Jain MK, Rumler D (1996) Method for making succinic acid, bacterial variants for use in the process, and methods for obtaining variants. Patent US5573931

    Google Scholar 

  • Guettler MV, Jain MK, Soni BK (1998) Process for making succinic acid, microorganisms for use in the process and methods of obtaining the microorganisms. Patent US5723322

    Google Scholar 

  • Guettler MV, Rumler D, Jain MK (1999) Actinobacillus succinogenes sp. nov., a novel succinic-acid-producing strain from the bovine rumen. Int J Syst Bacteriol 49:207–216

    Article  CAS  PubMed  Google Scholar 

  • Hodge DB, Andersson C, Berglund KA, Rova U (2009) Detoxification requirements for bioconversion of softwood dilute acid hydrolyzates to succinic acid. Enzyme Microb Technol 44:309–316

    Article  CAS  Google Scholar 

  • Hong YK, Hong WH, Chang HN (2000) Selective extraction of succinic acid from binary mixture of succinic acid and acetic acid. Biotechnol Lett 22:871–874

    Article  CAS  Google Scholar 

  • Inci I, Bayazit SS, Asci YS (2011) Separation of succinic acid from aqueous solution by alumina adsorption. J Chem Eng Data 56:4449–4453

    Article  CAS  Google Scholar 

  • Isar J, Agarwal L, Saran S, Kaushik R, Saxena RK (2007) A statistical approach to study the interactive effects of process parameters on succinic acid production from Bacteriodes fragilis. Anaerobe 13:50–56

    Article  CAS  PubMed  Google Scholar 

  • Isar J, Agarwal L, Saran S, Saxena RK (2006) Succinic acid production from Bacteroides fragilis: process optimization and scale up in a bioreactor. Anaerobe 12:231–237

    Article  CAS  PubMed  Google Scholar 

  • Jae J, Tompsett GA, Lin YC, Carlson TR, Shen JC, Zhang TY, Yang B, Wyman CE, Conner WC, Huber GW (2010) Depolymerization of lignocellulosic biomass to fuel precursors: maximizing carbon efficiency by combining hydrolysis with pyrolysis. Energy Environ Sci 3:358–365

    Article  CAS  Google Scholar 

  • Jantama K, Haupt MJ, Svoronos SA, Zhang X, Moore JC, Shanmugam KT, Ingram LO (2008) Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate. Biotechnol Bioeng 99:1140–1153

    Article  CAS  PubMed  Google Scholar 

  • Kim BS, Hong YK, Hong WH (2004a) Effect of salts on the extraction characteristics of succinic acid by predispersed solvent extraction. Biotechnol Bioprocess Eng 9:207–211

    Article  CAS  Google Scholar 

  • Kim DY, Yim SC, Lee PC, Lee WG, Lee SY, Chang HN (2004b) Batch and continuous fermentation of succinic acid from wood hydrolysate by Mannheimia succiniciproducens MBEL55E. Enzyme Microb Technol 35:648–653

    Article  CAS  Google Scholar 

  • Kim P, Laivenieks M, Vieille C, Zeikus JG (2004c) Effect of overexpression of Actinobacillus succinogenes phosphoenolpyruvate carboxykinase on succinate production in Escherichia coli. Appl Environ Microbiol 70:1238–1241

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Kuhnert P, Scholten E, Haefner S, Mayor D, Frey J (2010) Basfia succiniciproducens gen. nov., sp. nov., a new member of the family Pasteurellaceae isolated from bovine rumen. Int J Syst Evol Microbiol 60:44–50

    Article  CAS  PubMed  Google Scholar 

  • Lee SJ, Song H, Lee SY (2006) Genome-based metabolic engineering of Mannheimia succiniciproducens for succinic acid production. Appl Environ Microbiol 72:1939–1948

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Lee PC, Lee SY, Hong SH, Chang HN (2002) Isolation and characterization of a new succinic acid-producing bacterium, Mannheimia succiniciproducens MBEL55E, from bovine rumen. Appl Microbiol Biotechnol 58:663–668

    Article  CAS  PubMed  Google Scholar 

  • Lee PC, Lee SY, Hong SH, Chang HN, Park SC (2003) Biological conversion of wood hydrolysate to succinic acid by Anaerobiospirillum succiniciproducens. Biotechnol Lett 25:111–114

    Article  CAS  PubMed  Google Scholar 

  • Lee PC, Lee WG, Lee SY, Chang HN (2001) Succinic acid production with reduced by-product formation in the fermentation of Anaerobiospirillum succiniciproducens using glycerol as a carbon source. Biotechnol Bioeng 72:41–48

    Article  CAS  PubMed  Google Scholar 

  • Li Q, Wang D, Song ZY, Su ZG, Xing JM (2011) Integrated bioprocess for high-efficiency production of succinic acid in an expanded-bed adsorption system. Biochem Eng J 56:150–157

    Article  CAS  Google Scholar 

  • Li Q, Wang D, Song ZY, Zhou W, Wu Y, Xing JM, Su ZG (2010a) Dual-phase fermentation enables Actinobacillus succinogenes 130ZT to be a potential role for high-level lactate production from the bioresource. Bioresour Technol 101:7665–7667

    Article  CAS  PubMed  Google Scholar 

  • Li Q, Wang D, Wu Y, Li WL, Zhang YJ, Xing JM, Su ZG (2010b) One step recovery of succinic acid from fermentation broths by crystallization. Sep Purif Technol 72:294–300

    Article  CAS  Google Scholar 

  • Li Q, Wang D, Yang M, Wu Y, Li W, Xing J, Su Z (2010c) Kinetic evaluation of products inhibition to succinic acid producers Escherichia coli NZN111, AFP111 BL21 and Actinobacillus succinogenes 130ZT. J Microbiol 48:290–296

    Article  CAS  PubMed  Google Scholar 

  • Li Q, Xing JM, Li WL, Liu QF, Su ZG (2009) Separation of succinic acid from fermentation broth using weak alkaline anion exchange adsorbents. Ind Eng Chem Res 48:3595–3599

    Article  CAS  Google Scholar 

  • Li Q, Yang MH, Wang D, Li WL, Wu Y, Zhang YJ, Xing JM, Su ZG (2010d) Efficient conversion of crop stalk wastes into succinic acid production by Actinobacillus succinogenes. Bioresour Technol 101:3292–3294

    Article  CAS  PubMed  Google Scholar 

  • Li QA, Siles JA, Thompson IP (2010e) Succinic acid production from orange peel and wheat straw by batch fermentations of Fibrobacter succinogenes S85. Appl Microbiol Biotechnol 88:671–678

    Article  CAS  PubMed  Google Scholar 

  • Lin CSK, Du CY, Koutinas A, Wang R, Webb C (2008) Substrate and product inhibition kinetics in succinic acid production by Actinobacillus succinogenes. Biochem Eng J 41:128–135

    Article  CAS  Google Scholar 

  • Lin H, Bennett GN, San KY (2005) Fed-batch culture of a metabolically engineered Escherichia coli strain designed for high-level succinate production and yield under aerobic conditions. Biotechnol Bioeng 90:775–779

    Article  CAS  PubMed  Google Scholar 

  • Ling ETM, Dibble JT, Houston MR, Lockwood LB, Elliott LP (1978) Accumulation of 1-trans-2,3-epoxysuccinic acid and succinic acid by Paecilomyces varioti. Appl Environ Microbiol 35:1213–1215

    CAS  PubMed Central  PubMed  Google Scholar 

  • Liu YP, Zheng P, Sun ZH, Ni Y, Dong JJ, Zhu LL (2008) Economical succinic acid production from cane molasses by Actinobacillus succinogenes. Bioresour Technol 99:1736–1742

    Article  CAS  PubMed  Google Scholar 

  • Luque R, Lin CSK, Du C, Macquarrie DJ, Koutinas A, Wang R, Webb C, Clark JH (2009) Chemical transformations of succinic acid recovered from fermentation broths by a novel direct vacuum distillation–crystallisation method. Green Chem 11:193–200

    Article  CAS  Google Scholar 

  • Ma JF, Jiang M, Chen KQ, Xu B, Liu SW, Wei P, Hj Y, Chang HN, Ouyang PK (2011) Strategies for efficient repetitive production of succinate using metabolically engineered Escherichia coli. Bioproc Biosyst Eng 34:411–418

    Article  CAS  Google Scholar 

  • McKinlay JB, Shachar-Hill Y, Zeikus JG, Vieille C (2007) Determining Actinobacillus succinogenes metabolic pathways and fluxes by NMR and GC-MS analyses of C-13-labeled metabolic product isotopomers. Metab Eng 9:177–192

    Article  CAS  PubMed  Google Scholar 

  • Meynial-Salles I, Dorotyn S, Soucaille P (2008) A new process for the continuous production of succinic acid from glucose at high yield, titer, and productivity. Biotechnol Bioeng 99:129–135

    Article  CAS  PubMed  Google Scholar 

  • Nam HG, Park KM, Lim SS, Mun S (2011) Adsorption equilibria of succinic acid and lactic acid on Amberchrom CG300C resin. J Chem Eng Data 56:464–471

    Article  CAS  Google Scholar 

  • O’herrin SM, Kenealy WR (1993) Glucose and carbon dioxide metabolism by Succinivibrio dextrinosolvens. Appl Environ Microbiol 59:748–755

    PubMed Central  PubMed  Google Scholar 

  • Okino S, Inui M, Yukawa H (2005) Production of organic acids by Corynebacterium glutamicum under oxygen deprivation. Appl Microbiol Biotechnol 68:475–480

    Article  CAS  PubMed  Google Scholar 

  • Okino S, Noburyu R, Suda M, Jojima T, Inui M, Yukawa H (2008) An efficient succinic acid production process in a metabolically engineered Corynebacterium glutamicum strain. Appl Microbiol Biotechnol 81:459–464

    Article  CAS  PubMed  Google Scholar 

  • Orjuela A, Yanez AJ, Peereboom L, Lira CT, Miller DJ (2011) A novel process for recovery of fermentation-derived succinic acid. Sep Purif Technol 83:31–37

    Article  Google Scholar 

  • Otero JM, Cimini D, Patil KR, Poulsen SG, Olsson L, Nielsen J (2013) Industrial systems biology of Saccharomyces cerevisiae enables novel succinic acid cell factory. PLoS One 8:1–10

    Google Scholar 

  • Ponnampalam E (1999) Purification of organic acids using anion exchange chromatography. Patent WO9944707

    Google Scholar 

  • Raab AM, Gebhardt G, Bolotina N, Weuster-Botz D, Lang C (2010) Metabolic engineering of Saccharomyces cerevisiae for the biotechnological production of succinic acid. Metab Eng 12:518–525

    Article  CAS  PubMed  Google Scholar 

  • Samuelov NS, Datta R, Jain MK, Zeikus JG (1999) Whey fermentation by Anaerobiospirillum succiniciproducens for production of a succinatebased animal feed additive. Appl Environ Microbiol 65:2260–2263

    CAS  PubMed Central  PubMed  Google Scholar 

  • Schügerl K, Hubbuch J (2005) Integrated bioprocesses. Curr Opin Microbiol 8:294–300

    Article  PubMed  Google Scholar 

  • Song H, Lee JW, Choi S, You JK, Hong WH, Lee SY (2007) Effects of dissolved CO2 levels on the growth of Mannheimia succiniciproducens and succinic acid production. Biotechnol Bioeng 96:1296–1304

    Article  Google Scholar 

  • Song H, Lee SY (2006) Production of succinic acid by bacterial fermentation. Enzyme Microb Technol 39:352–361

    Article  CAS  Google Scholar 

  • Straathof AJJ, Efe Ç, Engel CAR, Garzon CSL, Van der Wielen LAM (2010) Fermentation and recovery of bio-based fumaric and succinic acid. J Biotechnol 150:82–83

    Article  Google Scholar 

  • Thakker C, Bhosale S, Ranade D (2006) Formation of succinic acid by Klebsiella pneumoniae MCM B-325 under aerobic and anaerobic conditions. J Microbiol Biotechnol 16:870–879

    CAS  Google Scholar 

  • Thakker C, Martínez I, San KY, Bennett GN (2012) Succinate production in Escherichia coli. Biotechnol J 7:213–224

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Urbance SE, Pometto AL, DiSpirito AA, Denli Y (2004) Evaluation of succinic acid continuous and repeat-batch biofilm fermentation by Actinobacillus succinogenes using plastic composite support bioreactors. Appl Microbiol Biotechnol 65:664–670

    Article  CAS  PubMed  Google Scholar 

  • Van der Werf MJ, Guettler MV, Jain MK, Zeikus JG (1997) Environmental and physiological factors affecting the succinate product ratio during carbohydrate fermentation by Actinobacillus sp. 130Z. Arch Microbiol 167:332–342

    Article  PubMed  Google Scholar 

  • Van Heerden CD, Nicol W (2013) Continuous and batch cultures of Escherichia coli KJ134 for succinic acid fermentation: metabolic flux distributions and production characteristics. Microb Cell Fact 12:80–90

    Article  PubMed Central  PubMed  Google Scholar 

  • Vemuri GN, Eiteman MA, Altman E (2002a) Effects of growth mode and pyruvate carboxylase on succinic acid production by metabolically engineered strains of Escherichia coli. Appl Environ Microbiol 68:1715–1727

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Vemuri GN, Eiteman MA, Altman E (2002b) Succinate production in dual-phase Escherichia coli fermentations depends on the time of transition from aerobic to anaerobic conditions. J Ind Microbiol Biotechnol 28:325–332

    Article  CAS  PubMed  Google Scholar 

  • Wang CX, Li Q, Tang H, Yan DJ, Zhou W, Xing JM, Wan YH (2012) Membrane fouling mechanism in ultrafiltration of succinic acid fermentation broth. Bioresour Technol 116:366–371

    Article  CAS  PubMed  Google Scholar 

  • Wang CX, Ming W, Yan DJ, Zhang CC, Yang MH, Liu YL, Zhang Y, Guo BH, Wan YH, Xing JM (2014) Novel membrane-based biotechnological alternative process for succinic acid production and chemical synthesis of bio-based poly (butylene succinate). Bioresour Technol 156:6–13

    Article  CAS  PubMed  Google Scholar 

  • Wang CX, Thygesen A, Liu YL, Li Q, Yang MH, Dang D, Wang Z, Wan YH, Lin WG, Xing JM (2013) Bio-oil based biorefinery strategy for the production of succinic acid. Biotechnol Biofuels 6:74–84

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Wang D, Li Q, Li W, Xing J, Su Z (2009) Improvement of succinate production by overexpression of a cyanobacterial carbonic anhydrase in Escherichia coli. Enzyme Microb Technol 45:491–497

    Article  CAS  Google Scholar 

  • Wang D, Li Q, Mao Y, Xing JM, Su ZG (2010) High-level succinic acid production and yield by lactose-induced expression of phosphoenol-pyruvate carboxylase in ptsG mutant Escherichia coli. Appl Microbiol Biotechnol 87:2025–2035

    Article  CAS  PubMed  Google Scholar 

  • Wang D, Li Q, Song ZY, Zhou W, Su ZG, Xing JM (2011a) High cell density fermentation via a metabolically engineered Escherichia coli for the enhanced production of succinic acid. J Chem Technol Biotechnol 86:512–518

    Article  CAS  Google Scholar 

  • Wang J, Zhu J, Bennett GN, San KY (2011b) Succinate production from different carbon sources under anaerobic conditions by metabolic engineered Escherichia coli strains. Metab Eng 13:328–335

    Article  CAS  PubMed  Google Scholar 

  • Wee YJ, Yun JS, Kang KH, Ryu HW (2002) Continuous production of succinic acid by a fumarate-reducing bacterium immobilized in a hollow-fiber bioreactor. Appl Biochem Biotechnol 98–100:1093–104

    Article  PubMed  Google Scholar 

  • Werpy T, Petersen G (2004) U.S. Department of Energy: Top value added chemicals from biomass Volume I—Results of screening for potential candidates from sugars and synthesis gas.

    Google Scholar 

  • Wieschalka S, Blombach B, Bott M, Eikmanns BJ (2012) Bio-based production of organic acids with Corynebacterium glutamicum. Microb Biotechnol 6:87–102

    Article  PubMed Central  PubMed  Google Scholar 

  • Yan DJ, Wanga CX, Zhou JM, Liu YL, Yang MH, Xing JM (2013) Construction of reductive pathway in Saccharomyces cerevisiae for effective succinic acid fermentation at low pH value. Bioresour Technol 156:232–239

    Article  Google Scholar 

  • Yedur S, Berglung KS, Dunuwila DD (2001) Succinic acid production and purification. Patent US6265190

    Google Scholar 

  • Yuzbashev TV, Yuzbasheva EY, Laptev IA, Sobolevskaya TI, Vybornaya TV, Larina AS (2011) Is it possible to produce succinic acid at a low pH? Bioeng Bugs 2:115–119

    Article  PubMed  Google Scholar 

  • Zhang XL, Jantama K, Moore JC, Jarboe LR, Shanmugam KT, Ingram LO (2009) Metabolic evolution of energy-conserving pathways for succinate production in Escherichia coli. Proc Natl Acad Sci USA 106:20180–20185

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Zheng P, Dong JJ, Sun ZH, Ni Y, Fang L (2009) Fermentative production of succinic acid from straw hydrolysate by Actinobacillus succinogenes. Bioresour Technol 100:2425–2429

    Article  CAS  PubMed  Google Scholar 

  • Zheng P, Fang L, Xu Y, Dong JJ, Ni Y, Sun ZH (2010) Succinic acid production from corn stover by simultaneous saccharification and fermentation using Actinobacillus succinogenes. Bioresour Technol 101:7889–7894

    Article  CAS  PubMed  Google Scholar 

  • Zhu NQ, Xia HH, Wang ZW, Zhao XM, Chen T (2013) Engineering of acetate recycling and citrate synthase to improve aerobic succinate production in Corynebacterium glutamicum. PLoS One 8:1–8

    Google Scholar 

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  1. National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, People’s Republic of China

    Qiang Li & Jianmin Xing

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  1. Qiang Li
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  1. FI Biopos e.V. and BTU Cottbus Research Center Teltow-Seehof, Teltow, Germany

    Birgit Kamm

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Li, Q., Xing, J. (2015). Microbial Succinic Acid Production Using Different Bacteria Species. In: Kamm, B. (eds) Microorganisms in Biorefineries. Microbiology Monographs, vol 26. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45209-7_7

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