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The Lotus japonicus Genome pp 229–243Cite as

TILLING in Lotus japonicus

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Part of the book series: Compendium of Plant Genomes ((CPG))

Abstract

Following the seminal work on TILLING in Arabidopsis thaliana, a population of EMS-mutagenized plants was established for Lotus japonicus ‘Gifu’ to be used for both forward and reverse screening. This was developed into the Lotus TILLING platform which subsequently became RevGenUK covering not only TILLING in L. japonicus, but also Medicago truncatula and other species. Over the last 10 years, nearly two thousand mutations for more than 160 genes have been identified using the platform. In this article, we cover the history, development and current technology employed in the process and explore the impact TILLING has had on Lotus research.

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Authors and Affiliations

  1. Metabolic Biology, John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK

    Trevor L. Wang & Fran Robson

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  1. Trevor L. Wang
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  2. Fran Robson
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Correspondence to Trevor L. Wang .

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Editors and Affiliations

  1. Kazusa DNA Research Institute, Kisarazu, Chiba, Japan

    Satoshi Tabata

  2. Department of Molecular Biology and Genetics, University of Aarhus, Aarhus, Denmark

    Jens Stougaard

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Wang, T.L., Robson, F. (2014). TILLING in Lotus japonicus . In: Tabata, S., Stougaard, J. (eds) The Lotus japonicus Genome. Compendium of Plant Genomes. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-44270-8_21

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