Abstract
We give an overview of confocal microscopy, discuss the physical principle and the achievable resolution. Different kinds of scanners are presented and some typical applications. In the second section we show, that a confocal measurement system can also be used to measure the surface profile of non-transparent objects. Finally, we discuss optical disc scanning systems.
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- 1.
Note If the diameter of the focus of the laser beam is less than the diameter of the pinhole, the pinhole lying at P can be omitted.
- 2.
There are materials that show inherently fluorescence. Others need to be colored with appropriate dyes.
References
M. Minsky, Microscopy Apparatus, U.S. Patent 3.013.467, applied for a patent 1957, granted 1961
T. Schwendt, R. Noll, Confocal microscopy, in Tailored Light 2, Laser Application Technology, Chapt. 19.4, ed. by R. Poprawe (Springer, Berlin Heidelberg, 2011), pp. 509–515. ISBN 978-3-642-01236-5
R.L. Price (ed.), W.G. Jerome (ed.), Basic Confocal Microscopy. (Springer, Heidelberg, 2011). ISBN 978-0387781747
J. Pawley, Handbook of Biological Confocal Microscopy (Springer, Heidelberg, 2006). ISBN 978-0387259215
W. Lukosz, Optical systems with resolving powers exceeding the classical limit. J. Opt. Soc. Am. 56, 1463–4172 (1966)
R.H. Webb, Confocal optical microscopy. Rep. Prog. Phys. 59, 427–471 (1996)
A. Donges, Grundlagen der konfokalen Laserscanning Mikroskopie. Laser J. 1(7), 32–33 (2007)
W. Denk, Two photon laser scanning microscopy. Science 248, 73–76 (1990)
Multiphoton Laser Scanning Mikroskopie, http://www.zeiss.de
C.H. Zander, J. Enderlein, R.A. Keller, Single Molecule Detection in Solution (Wiley-VCH, Berlin, 2002). ISBN 3-527-40310-8
T. Wilson, Confocal Microscopy (Academic Press, London, 1990). ISBN 978-0127572703
C. Sheppard, D. Shotton, C. Shepard, Laser Scanning Microscopy (Microscopy Handbooks) (Springer, Heidelberg, 1997). ISBN 978-0387915142
S. Wilhelm, B. Gröbler, M. Gluch, H. Heinz, Confocal Laser Scanning Microscopy-Principles, http://zeiss-campus.magnet.fsu.edu/referencelibrary/pdfs/ZeissConfocalPrinciples.pdf. Accessed 31 Oct 2013
M. Müller, Introduction to Confocal Microscopy (SPIE Press, Bellingham, 2006). ISBN 9780819460431
R. Leach, Optical Measurement of Surface Topography (Springer, Heidelberg, 2011). ISBN 978-3642120114
K. Stout, L. Blunt, Three-Dimensional Surface Topography (Penton, London, 2000). ISBN 9781857180268
K. Freischlad, Optical surface profiling: profilometer advances benefit surface analysis, film-thickness measurement. Laser FocusWorld 46(1), 77–81 (2010)
K. Pohlmann, Compact-Disc-Handbuch: Grundlagen des digitalen Audio, technischer Aufbau von CD-Playern, CD-I, Photo-CD (IWT-Verlag München, 1994). ISBN 3-88322-500-2
K. Clements, Understanding and Servicing CD Players (Elsevier Science, Oxford, 1998). ISBN 0-7506-0934-6
K. Pohlmann, The Compact Disc Handbook, 2nd edn. (A-R Editions, Middleton, 1992)
J. Braat, P. Dirksen, A. Janssen, Diffractive read-out of optical discs, chap. 4, in Optical Imaging and Microscopy, ed. by P. Török, F.-J. Kao. Springer Series in Optical Sciences, vol. 87 (Springer, Berlin, 2007), pp. 71–111
F. Zijp, Near-Field Optical Data Storage (Technische Universiteit Delft, Proefschrift, 2007)
G. Winkler, Tonaufzeichnung digital—moderne Audiotechnik mit Computerhilfe (Elektor Verlag, Aachen, 1990). ISBN 9783921608876
A. Donges, Wie funktionieren CD bzw. CD-Player? Praxis der Naturwissenschaften-Physik 6(48), 13–16 (1999)
Fraunhofer Institute for Laser Technology, Aachen, Sept 2008
E. Meinders, A. Mijiritskii, L. van Pieterson, M. Wuttig, Optical Data Storage—Phase-Change Media and Recording (Springer, New York, 2006). ISBN 978-1-4020-4216-4
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Donges, A., Noll, R. (2015). Confocal Measurement Systems. In: Laser Measurement Technology. Springer Series in Optical Sciences, vol 188. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-43634-9_12
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DOI: https://doi.org/10.1007/978-3-662-43634-9_12
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