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Investigating Adhesion Proteins by Single Cell Force Spectroscopy

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Book cover Novel Approaches for Single Molecule Activation and Detection

Part of the book series: Advances in Atom and Single Molecule Machines ((AASMM))

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Abstract

The ability to sense and measure adhesion forces by using force spectroscopy techniques has opened new perspectives in the field of mechanobiology. Single-cell force spectroscopy enables to directly measure interactive forces of single living cell with extracellular environment (i.e., cell, proteins and tissue) with extremely high resolution (single-protein level). Cell adhesion processes rely on the interaction of adhesion proteins with their environment. Cells sense and recognize the specific forces that are generated by the interaction with the environment, and transduce them into biochemical signals by which the cells evolve, move and grow. Single-cell force spectroscopy is the ideal tool to measure these forces and investigate the cellular response from its origin.

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Author information

Authors and Affiliations

  1. CBM S.c.r.l. Basovizza, Area Science Park, 34149, Trieste, Italy

    Laura Andolfi & Marco Lazzarino

  2. CNR-IOM Basovizza, Area Science Park, 34149, Trieste, Italy

    Laura Andolfi & Marco Lazzarino

Authors
  1. Laura Andolfi
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  2. Marco Lazzarino
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Correspondence to Laura Andolfi .

Editor information

Editors and Affiliations

  1. Department of Neuroscience and Neurotechnology, University of Genoa The Italian Institute of Technology, Genoa, Italy

    Fabio Benfenati

  2. Nanostructures Department, The Italian Institute of Technology, Genoa, Italy

    Enzo Di Fabrizio

  3. Neuroscience Area, Scuola Internazionale Superiore di Studi, Trieste, Italy

    Vincent Torre

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Andolfi, L., Lazzarino, M. (2014). Investigating Adhesion Proteins by Single Cell Force Spectroscopy. In: Benfenati, F., Di Fabrizio, E., Torre, V. (eds) Novel Approaches for Single Molecule Activation and Detection. Advances in Atom and Single Molecule Machines. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-43367-6_8

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