Skip to main content
SpringerLink
Log in

Modellstudien über virusbedingte Tumoren und deren immunologische Behandlung

  • Chapter
Der Mensch und sein Lebensraum

Part of the book series: Verhandlungen der Gesellschaft Deutscher Naturforscher und Ärzte ((NATURFORSCHER,volume 109. 1976))

Summary

After a review of the general biological properties of C-type oncorna viruses, results are presented on the structure of an exogenous murine leukemia virus (FLV) and on the serobiological properties of its structural proteins. Our findings suggested a major role of the viral surface glycoprotein gp71 in immunological defense mechanisms. This was confirmed by vaccination experiments with isolated gp71 in mice. The induced immunity was highly specific and not operative against endogenous murine C-viruses belonging to other serotypes. Surprisingly the latter were found to be activated by the vaccination with gp71 of FLV.

In heterologous animal species isolated FLV-gp71 induced the formation of broadly reacting antibodies. They were found to be effective in the therapy of infections with FLV in mice as well as with feline leukaemia virus in cats. Most impressive results were obtained with an antiserum prepared against feline leukaemia virus in a goat. This serum completely suppressed sarcomas induced by infection with feline sarcoma virus.

Zusammenfassung

Nach einer Übersicht über das allgemeine Verhalten der C-Typ-Onkorna-Viren werden die Ergebnisse eigener Untersuchungen über die Struktur eines exogenen Mäuse-Leukämie-Virus (FLV) und über die serobiologischen Eigenschaften seiner Strukturproteine geschildert. Die dabei gewonnenen Erkenntnisse ließen vermuten, daß das an der Virusoberfläche gelegene Glykoprotein gp71 eine wichtige Rolle bei der Immunabwehr spielt. Vakzinie-rungsversuche mit isoliertem Glykoprotein bei Mäusen bestätigten diese Vermutung. Sie zeigten aber auch, daß die entsprechende Immunantwort der Maus eine hohe Spezifität besitzt und sich nicht auf endogene C-Viren, die anderen Serotypen angehören, erstreckt; diese wurden vielmehr durch Impfung mit FLV gp71 aktiviert.

In anderen Tierarten veranlaßte dagegen isoliertes FLV gp71 die Bildung von breit reagierenden Antikörpern. Diese waren nicht nur bei FLV-infizierten Mäusen, sondern auch bei Infektionen von Katzen mit einem Katzen-Leukämie-Virus serotherapeutisch wirksam. Besonders eindrucksvolle Erfolge wurden bei der Behandlung von Virus-induzierten Sarkomen der Katze mit einem Antiserum erzielt, das gegen Katzen-Leukämie-Virus in einer Ziege hergestellt wurde.

Vortrag auf der 109. Versammlung der Gesellschaft Deutscher Naturforscher und Ärzte, Stuttgart 19.—23. September 1976

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 49.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 59.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. Ehrlich, P.: Experimentelle Carcinomstudien an Mäusen. Gesammelte Arbeiten, 2. Band: Immunitätslehre und Krebsforschung, Springer-Verlag Berlin, ed. F. Himmelweit (1957)

    Google Scholar 

  2. Schäfer, W.: Tumorviren — Eigenschaften und Bedeutung. Mitteilungen aus der MPG, Heft 5, S. 32Q (1974)

    Google Scholar 

  3. Temin, H.M., Mizutani, S.: RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature 226, 1211 (1970)

    Article  CAS  PubMed  Google Scholar 

  4. Baltimore, D.: RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature 226, 1209 (1970)

    Article  CAS  PubMed  Google Scholar 

  5. Möiling, K., Bolognesi, D.P., Bauer, H., Büsen, W., Plassman, H.W., Hausen, P.: Association of viral reverse transcriptase with an enzyme degrading the RNA moiety of RNA — DNA hybrids. Nature New Biol. 234, 240 (1971)

    Article  Google Scholar 

  6. Weiss, R.A., Friis, R.R., Katz, E., Vogt, P.K.: Induction of avian tumor viruses in normal cells by physical and chemical carcinogens. Virology 46, 920 (1971)

    Article  CAS  PubMed  Google Scholar 

  7. Rowe, W.P., Hartley, J.W., Lander, M.R., Pugh, W.E., Teich, N.: Noninfectious AKR mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia virus. Virology 46, 866 (1971)

    Article  CAS  PubMed  Google Scholar 

  8. Schäfer, W., Bolognesi, D.P.: Mammalian C-type oncornaviruses. Relationships between viral structural and cell surface antigens and their possible significance in immunological defense mechanism. Contemporary Topics in Immunobiology (im Druck)

    Google Scholar 

  9. Jarrett, W., Jarrett, O., Mackey, L., Laird, H., Hardy, W. jr., Essex, M.: Horizontal transmission of leukemia virus and leukemia in the cat. J. Natl. Cancer Inst. 51, 833 (1973)

    CAS  PubMed  Google Scholar 

  10. Benveniste, R.E., Todaro, G.J.: Evolution of type C viral genes. III. Preservation of ancestral murine type C viral sequences in pig cellular DNA. Proc. Nat. Acad. Sci. US 72, 4090 (1975)

    Article  CAS  Google Scholar 

  11. Moennig, V., Frank, H., Hunsmann, G., Ohms, P., Schwarz, H., Schäfer, W., Strandström, H.: C-type particles produced by a permanent cell line from a leukemic pig. II. Physical, chemical, and serological characterization of the particles. Virology 57, 179(1974)

    Article  CAS  PubMed  Google Scholar 

  12. Scolnick, E.M., Rands, E., Williams, D., Parks, W.P.: Studies on the nucleic acid sequences of Kirsten sarcoma virus: A model for formation of a mammalian RNA-containing sarcoma virus. J. Virol. 12, 458 (1973)

    CAS  PubMed Central  PubMed  Google Scholar 

  13. Ihle, J.N., Yurconic, M. jr., Hanna, M.G., jr.: Autogenous immunity to endogenous RNA tumor virus. J. Exptl. Med. 138, 194 (1973)

    Article  CAS  Google Scholar 

  14. Ihle J.N.. Hanna, M.G. jr., Schäfer W., Hunsmann, G., Bolo-gnesi, D.P.. Hüper, G.: Polypeptides of mammalian oncornaviruses. III. Localization of p15 and reactivity with natural antibody. Virology 63, 60 (1975)

    Article  CAS  PubMed  Google Scholar 

  15. Schäfer, W., Claviez, M., Frank, H., Hunsmann, G., Moennig, V., Schwarz H., Thiel, H.J., Bolognesi, D.P., Green, R.W., Langlois, A.J., Fischinger, P.J., de Noronha, F.: Mammalian C-type oncorna viruses. Relationships between structural virus and cell surface antigens and their possible significance in immunological defense mechanisms. Comparative Leukemia Research 1975. Bibl. Haematol. No. 43, ed. J. Clemmesen & D.S. Yohn, p. 88 (1976)

    Google Scholar 

  16. Green R.W., Bolognesi, D.P., Schäfer, W., Pister, L., Hunsmann G., deNoronha, F.: Polypeptides of mammalian oncornaviruses. I. Isolation and serological analysis of polypeptides from murine and feline C-type viruses. Virology 56, 565 (1973)

    Article  CAS  PubMed  Google Scholar 

  17. Hunsmann, G., Moennig, V., Pister, L., Seifert, E., Schäfer, W.: Properties of mouse leukemia viruses. VIII. The major viral glycoprotein of Friend leukemia virus. Seroimmunologi-cal, interfering and hemagglutinating capacities. Virology 62, 307 (1974)

    Article  CAS  PubMed  Google Scholar 

  18. Schäfer, W., Hunsmann, G., Moennig, V., de Noronha, F., Bolognesi, D.P., Green, R.W., Hüper, G.: Polypeptides of mammalian oncornaviruses. II. Characterization of a murine leukemia virus polypeptide (p15) bearing interspecies reactivity. Virology 63, 48 (1975)

    Article  Google Scholar 

  19. Fischinger, P.J., Schäfer, W., Bolognesi, D.P.: Neutralization of homologous and heterologous oncornaviruses by antisera against the p15 (E) and gp71 polypeptides of Friend murine leukemia virus. Virology 71, 169 (1976)

    Article  CAS  PubMed  Google Scholar 

  20. Bolognesi, D.P., Langlois, A.J., Schäfer, W.: Polypeptides of mammalian oncornaviruses. IV. Structural components of murine leukemia virus released as soluble antigens in cell culture. Virology 68, 550 (1975)

    Article  CAS  PubMed  Google Scholar 

  21. Hunsmann, G., Claviez, M., Moennig, V., Schwarz, H., Schäfer, W.: Properties of mouse leukemia viruses. X. Occurrence of viral structural antigens on the cell surface as revealed by a cytotoxicity test. Virology 69, 157 (1976)

    Article  CAS  PubMed  Google Scholar 

  22. Schwarz, H., Hunsmann, G., Moennig, V., Schäfer, W.: Properties of mouse leukemia viruses. XI. Immunoelectron microscopic studies on viral structural antigens on the cell surface. Virology 69, 169 (1976)

    Article  CAS  PubMed  Google Scholar 

  23. Grant, J.P., Bigner, D.D., Fischinger, P.J., Bolognesi, D.P.: Expression of murine leukemia virus structural antigens on the surface of chemically induced murine sarcomas. Proc. Nat. Acad. Sci. US 71, 5037 (1974)

    Article  CAS  Google Scholar 

  24. Kurth, R., Bauer, H.: Cell-surface antigens induced by avian RNA tumor viruses: Detection by a cytotoxic microassay. Virology 47, 426 (1972)

    Article  CAS  PubMed  Google Scholar 

  25. Gelderblom, H., Bauer, H., Graf, T.: Cell-surface antigens induced by avian RNA tumor viruses: Detection by immuno-ferritin technique. Virology 47, 416 (1972)

    Article  CAS  PubMed  Google Scholar 

  26. Hunsmann, G., Moennig, V., Seifert, E., Schäfer, W.: Schutz von Mäusen gegen Friend-Leukämie durch aktive bzw. passive Immunisierung mit isoliertem Virus-Glykoprotein bzw. seinem Antiserum. Z. Naturforsch. 30 c, 309 (1975)

    CAS  Google Scholar 

  27. Hunsmann, G., Moennig, V., Schäfer, W.: Properties of mouse leukemia viruses. IX. Active and passive immunization of mice against Friend leukemia with isolated viral GP71 glycoprotein and its corresponding antiserum. Virology 66, 327 (1975)

    Article  CAS  PubMed  Google Scholar 

  28. Ihle, J.N., Collins, J.J., Lee, J.C., Fischinger, P.J., Moennig, V., Schäfer, W., Hanna, M.G. jr., Bolognesi, D.P.: Characterization of the immune response to the major glycoprotein (gp71) of Friend leukemia virus. I. Response in Balb/c mice. Virology (im Druck)

    Google Scholar 

  29. Ihle, J.N., Lee, J.C., Collins, J.J., Fischinger, P.J., Pazmino, N.H., Moennig, V., Schäfer, W., Hanna, M.G. jr., Bolognesi, D.P.: Characterization of the immune response to the major glycoprotein (gp71) of Friend leukemia virus. II. Response in C57BL/6 mice. Virology (im Druck)

    Google Scholar 

  30. Ihle, J.N., Collins, J.J., Lee, J.C., Fischinger, P.J., Pazmino, N., Moennig, V., Schäfer, W., Hanna, M.G. jr., Bolognesi, D.P.: Characterization of the immune response to the major glycoprotein (gp71) of Friend leukemia virus. III. Influence on endogenous MuLV-mediated pathogenesis. Virology (im Druck)

    Google Scholar 

  31. Schäfer, W., Schwarz, H., Thiel, H.J., Wecker, E., Bolognesi, D.P.: Properties of mouse leukemia viruses. XIII. Serum-therapy of virus-induced murine leukemias. Virology (im Druck)

    Google Scholar 

  32. Rohrschneider, L.R., Kurth, R., Bauer, H.: Biochemical characterization of tumor-specific cell surface antigens on avian oncornavirus transformed cells. Virology 66, 481 (1975)

    Article  CAS  PubMed  Google Scholar 

  33. Springer, G.F., Desai, P.R., Scanlon, E.F.: Blood group MN precursors as human breast carcinoma-associated antigens and “naturally“ occurring human cytotoxins against them. Cancer 37, 169 (1976)

    Article  CAS  PubMed  Google Scholar 

  34. Bekesi, J.G., Holland, J.F., Fleminger, R. Yates, J., Henderson, E.S.: Immunotherapeutic efficacy of neuraminidase treated allogeneic myeloblasts in patients with acute myelocytic leukemia. Sabbatical Clinics of North America, Bd. 60, No. 3 (Mai 1976)

    Google Scholar 

  35. Sedlacek, H.H., Meesmann, H., Seiler, F.R.: Regression of spontaneous mammary tumors in dogs after injection of neura-minidase-treated tumor cells. Int. J. Cancer 15, 409 (1975)

    Article  CAS  PubMed  Google Scholar 

  36. Todaro, G.J.: Evolution and modes of transmission of RNA tumor viruses. American Journal of Pathology 81, 590 (1975)

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Max-Planck-Institut für Virusforschung, Spemannstraße 35/III, D-7400, Tübingen, Bundesrepublik Deutschland

    Professor Dr. Dr. h.c. W. Schäfer

Authors
  1. Professor Dr. Dr. h.c. W. Schäfer
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 1978 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Schäfer, W. (1978). Modellstudien über virusbedingte Tumoren und deren immunologische Behandlung. In: Der Mensch und sein Lebensraum. Verhandlungen der Gesellschaft Deutscher Naturforscher und Ärzte, vol 109. 1976. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-42998-3_17

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-42998-3_17

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-42721-7

  • Online ISBN: 978-3-662-42998-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation