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Metabolismus von Nitrosaminen in vivo I. Über die β-Oxidation aliphatischer Di-n-alkylnitrosamine: Die Bildung von 7-Methylguanin neben 7-Propyl- bzw. 7-Butylguanin nach Applikation von Di-n-propyl- oder Di-n-butylnitrosamin

  • F. W. Krüger

Zusammenfassung

Nach in vivo Applikation von 1-[14C]-Di-n-propyl- und 1-[14C]-Din-butylnitrosamin bei Ratten können 7-[14C]-n-Propyl- bzw. 7-[14C]-n-Butylguanin sowie 7-[14C]-Methylguanin in den Ribonucleinsäuren der Leber nachgewiesen werden. Nach Gabe von 2-[14C]-Di-n-propylnitrosamin war eine Bildung von [14C]-7-Methylguanin nicht nachweisbar. Es wurde im RNS-Hydrolysat nur 7-[14C]-n-Propylguanin gefunden. Aus diesen Befunden wird geschlossen, daß die Alkylketten von Di-n-Alkylnitrosaminen in vivo metabolisch zwischen dem α- und dem β-C-Atom unter Bildung einer methylierenden Verbindung gespalten werden.

On the Metabolism of Nitrosamines in vivo I. Evidence for β-Oxydation of Aliphatic Di-n-alkylnitrosamines: The Simultaneous Formation of 7-Methylguanine besides 7-Propyl- or 7-Butylguanine after Application of Di-n-Propyl- or Di-n-Butylnitrosamine

Summary

The in vivo application of 1-[14C]-di-n-propyl- and 1-[14C]-di-n-butylnitrosamine leads to formation of 7-[14C]-n-propyl- and 7-[14C]-n-butylguanine in the RNA of rat liver. 7-[14C]-methylguanine is simultaneously formed after application of both compounds. No labelled [14C]-7-methylguanine, however, could be detected when 2-[14C]-di-n-propylnitrosamines was applied. In this case only 7-[14C]-n-propylguanine was found. This leads to the conclusion that the aliphatic chains of di-n-alkylnitrosamines are metabolically split between the α- and the β-carbon during metabolism forming a methylating compound.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1971

Authors and Affiliations

  • F. W. Krüger
    • 1
    • 2
  1. 1.Institut für experimentelle Toxikologie und Chemotherapie am Deutschen Krebsforschungszentrum HeidelbergDeutschland
  2. 2.Institut für experimentelle Toxikologie und ChemotherapieHeidelbergDeutschland

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