Summary
The ATPase of myofibrils from normal and hypertrophied rat heart was studied in relation to the Ca2+-dependence of the reaction. The experimental data were fitted to theoretical curves based on the Hill equation. A positive cooperativity (n = 1.6) was found for the activation curve of the ATPase from normal heart. For hypertrophied heart no significant change in either the cooperativity or the Ca2+-sensitivity of troponin was detected. This indicates that the troponin-tropomyosin system is not altered during pressure-overload hypertrophy. However, it should be noted that no precautions were taken to control the state of phosphorylation of contractile proteins during the preparation of myofibrils.
Supported by the Deutsche Forschungsgemeinschaft
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Rupp, H. (1980). Cooperative effects of calcium on myofibrillar ATPase of normal and hypertrophied heart. In: Jacob, R. (eds) Experimental Cardiac Hypertrophy and Heart Failure. Steinkopff, Heidelberg. https://doi.org/10.1007/978-3-662-41468-2_22
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DOI: https://doi.org/10.1007/978-3-662-41468-2_22
Publisher Name: Steinkopff, Heidelberg
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