Experimental systolic and diastolic overloading in rats: total proteins turnover rate. Enzymatic and structural properties of myosin
The fractional turnover rate of the total cardiac proteins has been measured by using the continuous infusion technique with 3H lysine. It augments by a factor of 3 in systolic as well as in diastolic overloading, but in the former the peak was reached within the first week after operation and in the later the peak was not reached until the 14th day.
The myosin structure and enzymatic properties have been studied in several huge hypertrophic hearts (around 100% hypertrophy). In this condition the burst size of myosin is normal, as well as its K+ ATPase, but there is a sharp decline in the Ca2+ ATPase activity. Moreover, antibodies against native or defolded heavy meromyosin exhibit, a vertical shift in microcomplement fixation when made to react with molecules extracted from hypertrophied hearts. The normal isozymic pattern of rat heart myosin, as shown in non dissociating electrophoresis, was reversed.
KeywordsCatheter Phosphorus Hydration Filtration Phenol
Experimentelle systolische und diastolische Uberbelastung bei der Ratte: Umsatzrate der Gesamtproteine. Enzymatische und strukturelle Eigenschaften von Myosin
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- 2.Scharff R., I. G. Wool: Accumulation of amino acids in muscle of perfused heart. Effect of insulin. Biochem. J. 97, 257–271 (1965).Google Scholar
- 3.Blackburn, S.: The determination of amino acids by high-voltage electrophoresis. In: Methods in Biochemical Analysis, Glick ed., Interscience publ. vol. 13, p. 1–45 (New York 1965).Google Scholar
- 4.Burton, K.: Determination of DNA concentration with diphenylamine. In: Methods in Enzymology, Grossman et al. ed. Vol. 12 (B), p. 163–166 (New York 1968).Google Scholar
- 7.Munro, H. W., A. Fleck: The determination of nucleic acids. In: Methods in Biochemical Analysis, Glick ed. Intersciences pub. Vol. 14, p. 113–176 (New York 1965).Google Scholar
- 8.Cutiletta, A. F., R. Zak: Regression of myocardial hypertrophy. 1. Experimental model, changes in heart weight, nucleic acids and collagen. J. Molec. Cell. Cardiology. 7, 767–781 (1975).Google Scholar
- 10.Mavroudis, C., S. A. Jester, S. Jacobs, P. A. Ebert: Extra cellular space, water and ion concentration in the hypertrophied rat myocardium. Amer. J. Physiol. 236, 79–83 (1979).Google Scholar
- 11.Revis, N. W., A. J. V. Cameron: The relationship between fibrosis and lactate deshydrogenase isozymes in experimental hypertrophie heart of rabbits. Car diovascular Res. 12, 348–357 (1978).Google Scholar
- 14.Lompré, A. M., K. Schwartz, A. Albis, G Lacombe, N. V. Thiem, B. Swynghedauw: Myosin isoenzymic redistribution in chronic heart overload. Nature, in the pressGoogle Scholar
- 16.Schwartz, K., P. Bouveret, C. Sebag, Joe. Léger, B. Swynghedauw: Immunochemical evidence for the species specificity of heart myosin and heavy meromyosin. Bioch. Bioph. Acta. 435, 24–36 (1977).Google Scholar