Advertisement

Restriction Fragment Length Polymorphism of HLA-DR7 Alleles and Association with HLA-B Antigens

  • Elizabeth A. Bidwell
  • Jeffrey L. Bidwell
  • Trevor J. Jones
  • Peter T. Klouda
  • Ben A. Bradley
  • David A. Savage
  • Derek Middleton

Abstract

Although HLA-DR7 appears to be serologically homo- 2. geneous (1), it may be split according to epitopes recognized by T cells (Dw7, Dwl1) and is normally found in association with either DQw2 or DQw3. This report describes the correlation between restriction fragment 3. length polymorphism (RFLP) and phenotypic splits of DR7. Genomic DNAs from 74 DR7-positive individuals and from eight DR7-homozygous typing cells were analyzed by sequential hybridization with short (exonspecific) DRβ, DQβ, and DQα cDNA probes (J. L. Bidwell et al, this volume). Figure 1 shows the RFLP patterns revealed by each of these probes.

Keywords

Restriction Fragment Length Polymorphism Restriction Fragment Length Polymorphism Pattern Sequential Hybridization Author Affiliation International Histocompatibility Workshop 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Jakobsen BK, Terasaki PI, Svejgaard A. In: Albert ED, et al (eds): Histocompatibility Testing 1984. Berlin, Springer-Verlag, 1984, p 198.Google Scholar
  2. 2.
    Bidwell JL, Bidwell EA, Savage DA, Middleton D, Klouda PT, Bradley BA. A DNA-RFLP typing system which positively identifies serologically well-defined and ill-defined HLA-DR and DQ alleles, including DRw10. Transplantation 1988; 45: 640–646.PubMedCrossRefGoogle Scholar
  3. 3.
    Trucco M, Duquesnoy RJ. Polymorphism of the HLA-DQ subregion. Immunology Today 1986; 7: 297–300.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Elizabeth A. Bidwell
    • 1
  • Jeffrey L. Bidwell
    • 1
  • Trevor J. Jones
    • 1
  • Peter T. Klouda
    • 1
  • Ben A. Bradley
    • 1
  • David A. Savage
    • 2
  • Derek Middleton
    • 2
  1. 1.United Kingdom Transplant ServiceBristolEngland
  2. 2.Belfast City HospitalBelfast, Northern IrelandUK

Personalised recommendations