Restriction Fragment Length Polymorphism of HLA-DR7 Alleles and Association with HLA-B Antigens
Although HLA-DR7 appears to be serologically homo- 2. geneous (1), it may be split according to epitopes recognized by T cells (Dw7, Dwl1) and is normally found in association with either DQw2 or DQw3. This report describes the correlation between restriction fragment 3. length polymorphism (RFLP) and phenotypic splits of DR7. Genomic DNAs from 74 DR7-positive individuals and from eight DR7-homozygous typing cells were analyzed by sequential hybridization with short (exonspecific) DRβ, DQβ, and DQα cDNA probes (J. L. Bidwell et al, this volume). Figure 1 shows the RFLP patterns revealed by each of these probes.
KeywordsRestriction Fragment Length Polymorphism Restriction Fragment Length Polymorphism Pattern Sequential Hybridization Author Affiliation International Histocompatibility Workshop
- 1.Jakobsen BK, Terasaki PI, Svejgaard A. In: Albert ED, et al (eds): Histocompatibility Testing 1984. Berlin, Springer-Verlag, 1984, p 198.Google Scholar