Two Different T-Cell Perspectives on the DR1 Alloantigen
Exposure to thymic MHC molecules may influence the T-cell allorecognition repertoire. Therefore, since we have observed such a plethora of distinct fine specificities in alloreactive T-cell clones1,2, we postulated that T cells derived from allogeneic donors might recognize alloantigens with different fine specificities. To test this hypothesis, three series of alloreactive T-cell clones were derived by limiting dilution as previously described (1): series 61 (DR2;DRw13 anti-DR1); series 62 (DR2;DRw14 anti-DR1;DR2s); series 63 (DR2;DRw13 anti-DR1;DR2s). DR1-associated allodeterminants should have been primarily recognized because DQ and DP antigens were shared. However, T cells from series 62 would have differentiated in the presence of DR2 and DRw14, whereas those from series 61 and 63 would have developed in the presence of DR2 and DRw13.
KeywordsArthritis Titration Influenza Polypeptide Alanine
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