The H-2Kkml Mutation: A Single Nucleotide Substitution Causes Multiple Functional Differences in a Murine Class I MHC Molecule

  • J. M. Martinko
  • J. C. Solheim
  • J. Geliebter


The polymorphic MHC class I glycoproteins are implicated in a number of immunologic reactions involving T-cell recognition. Among these polymorphic molecules in the murine H-2 complex, a number of mutants have been identified. One of these mutants was mapped to the H-2K k allele in the CBA inbred mouse strain and resulted in graft rejection and graft versus host reactions. This mutant was designated H-2K kml and the mouse strain carrying it was designated CBA.M523 (1). Analysis of the structural variation in the H-2K kml molecule identified a single nucleotide substitution at codon 152, resulting in a change from Asp (GAT) in Kk to Ala (GCT) in K kml (2). This report documents the completion of the nucleotide sequence coding for the extracellular domains of the altered K kml and compares the known K k coding sequence from the AKR strain (3) to that of the CBA strain which is ancestral to the CBA.M523 mutant-bearing strain.


Single Nucleotide Substitution Mutant Cell Line Amino Acid Alteration Recognition Region eDNA Copy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Blandova Z, Mnatsakanyan YA, Egorov I. Study of H-2 mutations in mice. VI. M523, a new K end mutant. Immunogenetics 1975; 2: 291.CrossRefGoogle Scholar
  2. 2.
    Martinko JM, Solheim JC, Geliebter J. The H-2Kkml mutation: Nucleotide sequence and comparative analysis. Molec Immunol 1987; 24: 197.CrossRefGoogle Scholar
  3. 3.
    Arnold B, Burgert HG, Archibald AL, Kvist S. Complete nucleotide sequence of the H-2Kk gene. Comparison of three H-2K locus alleles. Nucleic Acids Res 1984; 24: 9473.CrossRefGoogle Scholar
  4. 4.
    Geliebter J, Zeff RA, Melvold RW, Nathenson SG. Mitotic recombination in germ cells generates two major histocompatibility complex mutant genes shown to be identical by RNA sequence analysis: Kbm9 and Kbmb. Proc Natl Acad Sci USA 1986; 83: 3371.PubMedCrossRefGoogle Scholar
  5. 5.
    Bjorkman PJ, Saper MA, Samroui B, Bennett WS, Strominger JC, Wiley DC. The foreign antigen binding site and T cell recognition regions of class I histocompatibility antigens. Nature 1987; 329: 512.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • J. M. Martinko
    • 1
  • J. C. Solheim
    • 1
  • J. Geliebter
    • 2
  1. 1.Department of MicrobiologySouthern Illinois UniversityCarbondaleUSA
  2. 2.The Rockefeller UniversityNew YorkUSA

Personalised recommendations