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TCR γ Chain Expression on Human Peripheral Blood T Lymphocytes

  • Frits Koning
  • Rafick P. Sekaly
  • Erwin Tschachler
  • Roberto Biassoni
  • Marvin S. ReitzJr.
  • Eric O. Long
  • John E. Coligan

Abstract

Expression of the human T-cell receptor (TCR) γ chain has been detected on subpopulations of peripheral T cells (1–5), a thymus-derived clone (6), and the T-cell line PEER (7). At least three forms of γ chains are known to exist in γδ TCRs (Table 1): [1] disulfide-linked Cγ1 chains have been detected on T-cell clones derived from the periphery (2–5,8); [2] a 55 kD Cγ2 chain, nondisulfide linked to a 40 kD δ chain is expressed on a cell line derived from an immunodeficiency patient (1) and on the PEER cell line (7); and [3] a second nondisulfide-linked ~ 41 kD Cγ2 chain has been found on a peripheral blood-derived cell line (8) and on a thymic clone (6). This latter receptor is poorly characterized and, furthermore, it is unknown if all three types of γ chains can be expressed by a single individual. Here we report that the 41 kD nondisulfide-linked γ chain can be separated into two components that are indistinguishable in charge, but differ slightly in size. This type of γ chain associates with a 36 kD putative δ chain. We show that all three forms of γδ TCRs can be expressed on CD3+WT31 lymphocytes from one individual. However, the 55 kD γ chain was not expressed by all individuals, which most likely reflects the absence of one of the Cγ2 alleles (9,10).

Keywords

Author Affiliation ET702 Cell Molecular Weight Difference Western Medical School 
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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Frits Koning
    • 1
  • Rafick P. Sekaly
    • 2
  • Erwin Tschachler
    • 3
  • Roberto Biassoni
    • 2
  • Marvin S. ReitzJr.
    • 3
  • Eric O. Long
    • 2
  • John E. Coligan
    • 1
  1. 1.Biological Resources BranchNational Institutes of HealthBethesdaUSA
  2. 2.Laboratory of Immunogenetics, National Institute of Allergy and Infectious DiseasesNational Institutes of HealthBethesdaUSA
  3. 3.Laboratory of Tumor Cell Biology, National Cancer InstituteNational Institutes of HealthBethesdaUSA

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